Plant transposable elements : methods and protocols
著者
書誌事項
Plant transposable elements : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1057）（Springer protocols）
Humana Press, c2013
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Transposable elements have played a major role in shaping plant genome structure and gene expression. Transposons not only drive sequence expansion, induce mutations and generate chromosome rearrangements, they also help to shape the epigenetic topology of the eukaryotic genome. In Plant Transposable Elements: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study transposons. These methods include computational approaches to study the ancient transposon remnants that comprise the bulk of plant genomes, as well as laboratory techniques to identify recent and ongoing transposition events. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Transposable Elements: Methods and Protocols seeks to aid scientists in the further study of transposons by providing essential background information and specific experimental protocols.
目次
1. Historical Overview of Transposable Element Research
Peter A Peterson
2. Distinguishing Variable Phenotypes from Variegation Caused by Transposon Activities
Virginia Walbot
3. Using Transposons for Genetic Mosaic Analysis of Plant Development
Philip W. Becraft
4. Survey of Natural and Transgenic Gene Markers Used to Monitor Transposon Activity
Lakshminarasimhan Krishnaswamy, Thomas Peterson
5. Molecular Biology of Maize Ac/Ds Elements - an Overview
Katina Lazarow, My-Linh Du and Reinhard Kunze
6. Gene Tagging with Engineered Ds Elements in Maize
Yubin Li, Gregorio Segal, Qinghua Wang, and Hugo K. Dooner
7. Plant Regeneration Methods for Rapid Generation of a Large Scale of Ds Transposant Population in Rice
Yuan Hu Xuan, Jin Huang, Gihwan Yi, Dong-Soo Park, Soo Kwon Park, Moo Young Eun, Doh Won Yun, Gang-Seob Lee, Tae Ho Kim, Chang-deok Han
8. Isolation of Sequences Flanking Ac Insertion Sites by Ac Casting
Dafang Wang and Thomas Peterson
9. Regulation of the Mutator System of Transposons in Maize
Damon Lisch
10. Using MuDR/Mu Transposons in Directed Tagging Strategies
Virginia Walbot and Julia Questa
11. Genetic and Molecular Analysis of UniformMu Transposon Insertion Lines
Donald R. McCarty, Masaharu Suzuki, Charles Hunter, Wayne T. Avigne, and Karen E. Koch
12. Digestion-Ligation-Amplification (DLA): A Simple Genome Walking Method to Amplify Unknown Sequences Flanking Mutator (Mu) Transposons and Thereby Facilitate Gene Cloning
Sanzhen Liu, An-Ping Hsia, Patrick S. Schnable
13. Molecular Genetics and Epigenetics of CACTA Elements
Nina V. Fedoroff
14. Activation Tagging Using the Maize En-I Transposon System for the Identification of Abiotic Stress Resistance Genes in Arabidopsis
Amal Harb and Andy Pereira
15. Reverse Genetics in Rice using Tos17Delphine Mieulet, Anne Dievart, Gaetan Droc, Nadege Lanau and Emmanuel Guiderdoni
16. Identification and Applications of the Petunia Class II Act1/dTph1 Transposable Element System Tom Gerats , Jan Zethof, and Michiel Vandenbussche
17. Transposon Display: A Versatile Method for Transposon Tagging
Michiel Vandenbussche, Jan Zethof, and Tom Gerats
18. Massive Indexed Parallel Identification of Transposon Flanking Sequences
Michiel Vandenbussche, Jan Zethof, and Tom Gerats
19. Use of Next Generation Sequencing (NGS) Technologies for the Genome-wide Detection of Transposition
Moaine Elbaidouri, Cristian Chaparro and Olivier Panaud
20. Overview of Repeat Annotation and de novo Repeat Identification
Ning Jiang
21. Computational Methods for Identification of DNA Transposons
Ning Jiang
22. TEnest 2.0: Computational Annotation and Visualization of Nested Transposable Elements
Brent A. Kronmiller and Roger P. Wise
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