Helicobacter pylori : methods and protocols

著者

• Smith, Sinead M.

書誌事項

Helicobacter pylori : methods and protocols

edited by Sinéad M. Smith

（Methods in molecular biology / John M. Walker, series editor, 2283）（Springer protocols）

Humana Press, c2021

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This book brings together a collection of methods for the successful detection, culture and investigation of H. pylori, and its associated diseases. Chapters guide the reader through overviews on H. pylori infection, protocols for analyzing human clinical samples, and protocols for characterizing H. pylori, its functional components and its interactions with host cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Helicobacter pylori: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.

目次

1. An Overview of Helicobacter pylori Infection Rebecca FitzGerald and Sinead Smith 2. The Urea Breath Test for the Non-Invasive Detection of Helicobacter pylori Anthony O'Connor 3. Real-time Diagnosis of Helicobacter pylori during Endoscopy by Gastric Juice Analysis Cristiano Spada and Stefania Piccirelli 4. Molecular Detection of Antibiotic-resistant Helicobacter pylori Denise Brennan, Colm O'Morain, Deirdre McNamara, and Sinead Smith 5. Endoscopic Detection of Helicobacter pylori by the Rapid Urease Test Vikrant Parihar and Deirdre McNamara 6. Culture-based Antimicrobial Susceptibility Testing for Helicobacter pylori Philippe Lehours and Francis Megraud 7. Purification of Total RNA from Stomach Tissue Biopsies Rebecca FitzGerald, Malini Devapal, John J. Hickey, Dermot Kelleher, and Sinead Smith 8. Gene Expression Analysis by Reverse Transcription Quantitative PCR Eva M. Campion and Sinead T. Loughran 9. A Method to Investigate the Helicobacter pylori-associated DNA Methylome Hae Dong Woo and Zdenko Herceg 10. Profiling the Gastrointestinal Microbiota Brunella Posteraro, Flavio De Maio, and Antonio Gasbarrini 11. Helicobacter pylori Virulence Factor Genotyping Rebecca FitzGerald, Chavi Sinha, Abbas Yadegar, and Sinead Smith 12. Generation of Isogenic Mutant Strains of Helicobacter pylori Stephanie L. Servetas, Jeannette M. Whitmire, and D. Scott Merrell 13. Isolation of Outer Membrane Vesicles from Helicobacter pylori Henry J. Windle 14. A Mouse Model of Helicobacter pylori Infection Tanmoy Kumar Dey, Bipul Chandra Karmakar, Avijit Sarkar, Sangita Paul, and Asish Kumar Mukhopadhyay 15. Investigating the Interaction of Helicobacter pylori with the Gastric Mucosa Marguerite Clyne, Ciara Dunne, and Brendan Dolan 16. Evaluating Host Responses to Helicobacter pylori using ELISA and Western Blot Ciara Nulty, Gillian Barber, Ewelina Flis, and Emma M. Creagh 17. Analysis of Innate Immune Responses to Helicobacter pylori Michelle Chonwerawong and Richard L. Ferrero 18. Analysis of T Cell Responses to Helicobacter pylori Infection Kazuyo Kaneko and Karen Robinson 19. Miniaturised In Vitro Assays to Study Cellular Phenotypic Characteristics: Proliferation, Migration, Invasion, and Anoikis-resistance Niamh McNamee and Lorraine O'Driscoll

「Nielsen BookData」 より

詳細情報

• NII書誌ID(NCID): BC15047367
• ISBN:
  • 9781071613016
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xi, 234 p.
• 大きさ: 27 cm
• 分類:
  • DC23 : 616.33014
• 件名:
  • LCSH : Helicobacter pylori infections
  • LCSH : Helicobacter pylori
• 親書誌ID:
  • BA10794673
  • BA88873632