Candida species : methods and protocols
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書誌事項
Candida species : methods and protocols
（Methods in molecular biology, 2542）
Springer, 2022
Second edition
- hbk.
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内容説明・目次
内容説明
This updated volume explores Candida and candidiasis methods, useful to a wide variety of Candida scientists including those new to the field. Beginning with a section on the Candida genome, the book continues by covering techniques for antifungal discovery and studying resistance, pathogenesis and virulence, communities, as well as immunity. Biofilm protocols are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Candida Species: Methods and Protocols, Second Edition serves as an ideal guide for researchers working to further our understanding of this family of infective fungi.
目次
Part I: The Candida Genome
1. CRISPR-Mediated Genome Editing in the Human Fungal Pathogen C. albicans
Ben A. Evans and Douglas A. Bernstein
2. Plasmid-Based CRISPR-Cas9 Editing in Multiple Candida Species
Lisa Lombardi and Geraldine Butler
3. A Proteomic Approach for the Quantification of Post-Translational Protein Lysine Acetylation in Candida albicans
Raju Shivarathri, Manju Chauhan, Rounik Mazumdar, Phan Canh Trinh, Wolfgang Reiter, Markus Hartl, Karl Kuchler, and Neeraj Chauhan
4. How to Use the Candida Genome Database
Marek S. Skrzypek, Jonathan Binkley, and Gavin Sherlock
5. Sub-Telomeric Chromatin Structure by Chromosome Conformation Capture (3C)-qPCR Methodology in Candida glabrata
Eunice Lopez-Fuentes, Grecia Hernandez-Hernandez, Alejandro De Las Penas, and Irene Castano
6. Construction of Double Heterozygous Deletion Strains for Complex Haploinsufficiency-Based Genetic Analysis in Candida albicans
Virginia Glazer and Damian Krysan
Part II: Antifungal Discovery and Resistance
7. Using Synthetic Genetic Interactions in Candida glabrata as a Novel Method to Detect Genes with Roles in Antifungal Drug Resistance
Jane Usher
8. Identifying Specific Small Molecule-Protein Interactions Using Target Abundance-Based Fitness Screening (TAFiS)
Jessica Regan and Glen E. Palmer
9. High Throughput Phospholipidomics of Candida Cells: From Sample Preparation to Data Analysis
Mohit Kumar, Rajendra Prasad, and Ashutosh Singh
10. Genetic Screens of an Anti-Candida Natural Product Using the Heterozygous Saccharomyces cerevisiae Mutant Library
Lulu Zhang, Dongmei Li, Richard Calderone, and Xiaodong She
11. Assessing Complex I (CI) Mitochondrial Subunit Protein Functions in Candida albicans
Dongmei Li, Xiaodong She, and Richard Calderone
Part III: Pathogenesis and Virulence
12. In Vitro Biophysical Characterization of Candidalysin: A Fungal Peptide Toxin
Sejeong Lee, Nessim Kichik, Olivia W. Hepworth, Jonathan P. Richardson, and Julian R. Naglik
13. Assessment of cAMP-PKA Signaling in Candida glabrata by FRET-Based Biosensors
Liesbeth Demuyser, Wouter Van Genechten, and Patrick Van Dijck
14. Methods Related to the Immunopathogenesis of Vulvovaginal Candidiasis and Associated Neutrophil Anergy
Junko Yano and Paul L. Fidel, Jr.
15. A Simple Method for Growth of Candida albicans Biofilms under Continuous Media Flow and for Recovery of Biofilm Dispersed Cells
Priya Uppuluri
16. A Simple 96 Well Plate-Based Method for Development of Candida Biofilms under Static Conditions
Priya Uppuluri
17. Comparative Analysis of the Fitness of Candida albicans Strains during Colonization of the Mice Gastrointestinal Tract
Daniel Prieto and Jesus Pla
18. Detection and Identification of Candida auris from Clinical Skin Swabs
Milena Kordalewska and David S. Perlin
19. Production and Isolation of the Candida Species Biofilm Extracellular Matrix
Marienella Hereidia and David Andes
Part IV: Communities
20. Characterization of the Effects of Candida Gastrointestinal Colonization on Clostridioides difficile Infection in a Murine Model
Jesus A. Romo and Carol A. Kumamoto
21. Analysis of Gut Microbiome Using Fecal Samples
Ahmed Gamal, Mayyadah Alabdely, Kelly Steller, Hilmi Al-Shakhir, Mohammed Elshaer, and Mahmoud Ghannoum
22. Measurements of Treg Cell Induction by Candida albicans DNA Using Flow Cytometry
Dongmei Li, Jie Cheng, Richard Calderone, and Joseph A. Bellanti
Part V: Immunity
23. Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure
Ebrima Bojang, Rebecca Drummond, and Rebecca A. Hall
24. Isolation, Physicochemical Characterization, Labeling, and Biological Evaluation of Mannans and Glucans
Michael D. Kruppa, Douglas W. Lowman, Harry E. Ensley, Zuchao Ma, Bridget Graves, Jennifer Kintner, Jennifer V. Hall, Tammy R. Ozment, and David L. Williams
25. Understanding the Protective Role of IL-17 during Oropharyngeal Candidiasis
Jessica Saul-McBeth and Heather R. Conti
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