CiNii 図書 - Candida species : methods and protocols

This updated volume explores Candida and candidiasis methods, useful to a wide variety of Candida scientists including those new to the field. Beginning with a section on the Candida genome, the book continues by covering techniques for antifungal discovery and studying resistance, pathogenesis and virulence, communities, as well as immunity. Biofilm protocols are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Candida Species: Methods and Protocols, Second Edition serves as an ideal guide for researchers working to further our understanding of this family of infective fungi.

Part I: The Candida Genome 1. CRISPR-Mediated Genome Editing in the Human Fungal Pathogen C. albicans Ben A. Evans and Douglas A. Bernstein 2. Plasmid-Based CRISPR-Cas9 Editing in Multiple Candida Species Lisa Lombardi and Geraldine Butler 3. A Proteomic Approach for the Quantification of Post-Translational Protein Lysine Acetylation in Candida albicans Raju Shivarathri, Manju Chauhan, Rounik Mazumdar, Phan Canh Trinh, Wolfgang Reiter, Markus Hartl, Karl Kuchler, and Neeraj Chauhan 4. How to Use the Candida Genome Database Marek S. Skrzypek, Jonathan Binkley, and Gavin Sherlock 5. Sub-Telomeric Chromatin Structure by Chromosome Conformation Capture (3C)-qPCR Methodology in Candida glabrata Eunice Lopez-Fuentes, Grecia Hernandez-Hernandez, Alejandro De Las Penas, and Irene Castano 6. Construction of Double Heterozygous Deletion Strains for Complex Haploinsufficiency-Based Genetic Analysis in Candida albicans Virginia Glazer and Damian Krysan Part II: Antifungal Discovery and Resistance 7. Using Synthetic Genetic Interactions in Candida glabrata as a Novel Method to Detect Genes with Roles in Antifungal Drug Resistance Jane Usher 8. Identifying Specific Small Molecule-Protein Interactions Using Target Abundance-Based Fitness Screening (TAFiS) Jessica Regan and Glen E. Palmer 9. High Throughput Phospholipidomics of Candida Cells: From Sample Preparation to Data Analysis Mohit Kumar, Rajendra Prasad, and Ashutosh Singh 10. Genetic Screens of an Anti-Candida Natural Product Using the Heterozygous Saccharomyces cerevisiae Mutant Library Lulu Zhang, Dongmei Li, Richard Calderone, and Xiaodong She 11. Assessing Complex I (CI) Mitochondrial Subunit Protein Functions in Candida albicans Dongmei Li, Xiaodong She, and Richard Calderone Part III: Pathogenesis and Virulence 12. In Vitro Biophysical Characterization of Candidalysin: A Fungal Peptide Toxin Sejeong Lee, Nessim Kichik, Olivia W. Hepworth, Jonathan P. Richardson, and Julian R. Naglik 13. Assessment of cAMP-PKA Signaling in Candida glabrata by FRET-Based Biosensors Liesbeth Demuyser, Wouter Van Genechten, and Patrick Van Dijck 14. Methods Related to the Immunopathogenesis of Vulvovaginal Candidiasis and Associated Neutrophil Anergy Junko Yano and Paul L. Fidel, Jr. 15. A Simple Method for Growth of Candida albicans Biofilms under Continuous Media Flow and for Recovery of Biofilm Dispersed Cells Priya Uppuluri 16. A Simple 96 Well Plate-Based Method for Development of Candida Biofilms under Static Conditions Priya Uppuluri 17. Comparative Analysis of the Fitness of Candida albicans Strains during Colonization of the Mice Gastrointestinal Tract Daniel Prieto and Jesus Pla 18. Detection and Identification of Candida auris from Clinical Skin Swabs Milena Kordalewska and David S. Perlin 19. Production and Isolation of the Candida Species Biofilm Extracellular Matrix Marienella Hereidia and David Andes Part IV: Communities 20. Characterization of the Effects of Candida Gastrointestinal Colonization on Clostridioides difficile Infection in a Murine Model Jesus A. Romo and Carol A. Kumamoto 21. Analysis of Gut Microbiome Using Fecal Samples Ahmed Gamal, Mayyadah Alabdely, Kelly Steller, Hilmi Al-Shakhir, Mohammed Elshaer, and Mahmoud Ghannoum 22. Measurements of Treg Cell Induction by Candida albicans DNA Using Flow Cytometry Dongmei Li, Jie Cheng, Richard Calderone, and Joseph A. Bellanti Part V: Immunity 23. Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure Ebrima Bojang, Rebecca Drummond, and Rebecca A. Hall 24. Isolation, Physicochemical Characterization, Labeling, and Biological Evaluation of Mannans and Glucans Michael D. Kruppa, Douglas W. Lowman, Harry E. Ensley, Zuchao Ma, Bridget Graves, Jennifer Kintner, Jennifer V. Hall, Tammy R. Ozment, and David L. Williams 25. Understanding the Protective Role of IL-17 during Oropharyngeal Candidiasis Jessica Saul-McBeth and Heather R. Conti

「Nielsen BookData」より

Candida species : methods and protocols

著者

書誌事項

大学図書館所蔵全1件

この図書・雑誌をさがす

内容説明・目次

詳細情報

書き出し