Mitophagy : methods and protocols
Author(s)
Bibliographic Information
Mitophagy : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1759）（Springer protocols）
Humana Press, c2018
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This volume focuses on mitophagy providing methods and protocols on the methodologies and the background information on monitoring mitophagy. Chapters detail the analysis of mitophagy in connection with Parkinson's disease, especially young onset Parkinson's disease impaired by PINK/parkin pathway, Drosophila, yeast, and Caenorhabditis elegans models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mitophagy: Methods and Protocols aims to stimulate and encourage researchers to carry out ongoing and new investigations into mitophagy.
Table of Contents
Part I: PINK 1/Parkin-mediated Mitophagy
1. Short Overview of PINK1/Parkin-mediated Mitophagy
Norihiko Furuya
2. Induction of PINK1/Parkin-Mediated Mitophagy
Shigeto Sato And Norihiko Furuya
3. Immunocytochemical Monitoring of PINK1/Parkin-mediated Mitophagy in Cultured Cells
Motoki Fujimaki, Shinji Saiki, Yukiko Sasazawa, Kei-Ichi Ishikawa, Yoko Imamichi, Katsuhiko Sumiyoshi, and Nobutaka Hattori
4. The Use Of Correlative Light-electron Microscopy (CLEM) To Study PINK1/Parkin-mediated Mitophagy
Folma Buss and Chieko Kishi-Itakura
5. Observation Of Parkin-mediated Mitophagy in Pancreatic Beta-cells
Atsushi Hoshino and Satoaki Matoba
6. Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila
Tsuyoshi Inoshita, Kahori Shiba-Fukushima, Hongrui Meng, Nobutaka Hattori, and Yuzuru Imai
7. Assessment of Mitophagy in iPS Cell-derived Neurons
Kei-Ichi Ishikawa, Akihiro Yamaguchi, Hideyuki Okano, and Wado Akamatsu
PART II: Mitophagy Associated with Nix
8. Investigation of Yeast Mitophagy with Fluorescence Microscopy and Western Blotting
Sachiyo Nagumo And Koji Okamoto
9. Mitopho8 60 Assay as a Tool to Quantitatively Measure Mitophagy Activity
Zhiyuan Yao, Xu Liu And Daniel J. Klionsky
10. Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants
Kentaro Furukawa and Tomotake Kanki
11. Flow Cytometer Monitoring of Bnip3 and Bnip3L/Nix-dependent Mitophagy
Matilda Sprung, Ivan Dikic, and Ivana Novak
12. Exploring Micrornas on Nix-Dependent Mitophagy
Wen Li, Hao Chen, Shupeng Li, Guanghong Lin, and Du Feng
Part III: Others
13. Monitoring of Atg5-Independent Mitophagy
Satoko Arakawa, Shinya Honda, Satoru Torii, Masatsune Tsujioka, and Shigeomi Shimizu
14. Monitoring of Paternal Mitochondrial Degradation In Caenorhabditis Elegans
Ken Sato and Miyuki Sato
15. Detection of Hypoxia-induced and Iron Depletion-induced Mitophagy in Mammalian Cells
Shun-Ichi Yamashita and Tomotake Kanki
16. Monitoring Mitophagy during Ageing in Caenorhabditis Elegans
Nikolaos Chrmpilas, Konstantinos Kounakis, and Nektarios Tavernarakis
17. Iron-Starvation Induced Mitophagy in Yeast
Koichi Tanabe
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