Rhodopsin : methods and protocols

Author(s)

    • Gordeliy, Valentin

Bibliographic Information

Rhodopsin : methods and protocols

edited by Valentin Gordeliy

(Methods in molecular biology / John M. Walker, series editor, 2501)(Springer protocols)

Humana Press, c2022

  • : [hardback]

Available at  / 3 libraries

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Note

Includes bibliographical references and index

Description and Table of Contents

Description

This volume provides readers with the latest information on the advances made in the field of rhodopsins. The chapters in this book cover topics such as new discoveries and developments; new ways to search for rhodopsins; methods to characterize the function and structure of rhodopsins on a molecular level; nano volume high throughput in meso crystallization, and fourth generation x-ray synchrotron sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Rhodopsin: Methods and Protocols is a valuable resource for any scientist and researcher interested in learning more about this developing field.

Table of Contents

Preface... Table of Contents... Contributing Authors... 1. Microbial Rhodopsins Valentin Gordeliy, Kirill Kovalev, Ernst Bamberg, Francisco Rodrigez-Valera, Dmitrii Zabelskii, Alexey Alekseev, Riccardo Rosselli, Ivan Gushchin, and Ivan Okhrimenko 2. Molecular Biology of Microbial Rhodopsins Martin Engelhard 3. Rhodopsin-Based Optogenetics: Basics and Applications Alexey Alekseev, Valentin Gordeliy, and Ernst Bamberg 4. Searching Metagenomes for New Rhodopsins Francisco Rodriguez Valera, Alina Pushkarev, Riccardo Rosselli, and Oded Beja 5. E. coli Expression and Purification of Microbial and Viral Rhodopsins Taras Balandin, Dmitro Volkov, Alexey Alexeev, Kirill Kovalev, Dmitry Bratanov, and Valentin Gordeliy 6. Crystallization of Microbial Rhodopsins Kirill Kovalev, Roman Astashkin, Valentin Gordeliy, and Vadim Cherezov 7. Crystallographic Studies of Rhodopsins: Structure and Dynamics Marie Luise Grunbein, Gabriela Nass Kovacs, Marco Kloos, Alexander Gorel, R. Bruce Doak, Robert L. Shoeman, Thomas R.M. Barends, and Ilme Schlichting 8. Time-Resolved UV-VIS Spectroscopy of Microbial Rhodopsins Dmytro Soloviov, Valentin Borshchevskiy, and Igor Chizhov 9. Solid-State NMR Spectroscopy on Microbial Rhodopsins Clara Nassrin Kriebel, Johanna Becker-Baldus, and Clemens Glaubitz 10. FTIR and Raman Spectroscopy of Rhodopsins Hideki Kandori and Yasuhisa Mizutani 11. Single Cell Resolution Optogenetics via Expression of Soma-Targeted Rhodopsins Changyang Linghu, I-Wen Chen, Dimitrii Tanese, Valeria Zampini, and Or A. Shemesh 12. Electrophysiological Characterization of Microbial Rhodopsins Transport Properties: Electrometric and pH Measurements using Planar Lipid Bilayer, Collodion Film and Fluorescent Probes Approaches Tatiana I. Rokitskaya, Nina L. Maliar, Sergey A. Siletsky, Valentin Gordeliy, and Yuri N. Antonenko 13. Electrophysiological Characterization of Microbial Rhodopsins by Patch-Clamp Experiments Thomas Mager 14. Molecular Optimization of Rhodopsin-Based Tools for Neuroscience Applications Lief E. Fenno, Rivka Levy, and Ofer Yizhar 15. Optogenetic Studies of Mitochondria Kai Chen, Patrick Ernst, Xiaoguang (Margaret) Liu, and Lufang Zhou 16. In Vivo and In Vitro Characterization of Cyclase and Phosphodiesterase Rhodopsins Yuehui Tian, Shiqiang Gao, and Georg Nagel 17. Optogenetic Control of Human Stem Cell-Derived Neurons Rouhollah Habibey, Johannes Striebel, Kritika Sharma, and Volker Busskamp Subject Index List...

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Details

  • NCID
    BC16332438
  • ISBN
    • 9781071623282
  • Country Code
    us
  • Title Language Code
    eng
  • Text Language Code
    eng
  • Place of Publication
    New York
  • Pages/Volumes
    xi, 363 p.
  • Size
    26 cm
  • Classification
  • Subject Headings
  • Parent Bibliography ID
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