Plant gene silencing : methods and protocols
著者
書誌事項
Plant gene silencing : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2408）（Springer protocols）
Humana Press, c2022
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This second edition provides a comprehensive review of various gene silencing methodologies and applications. Chapters detail a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS), understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, artificial miRNAs for gene silencing, and high throughput RNAi. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Plant Gene Silencing: Methods and Protocols, Second Edition aims to further the understanding of functional relevance of target genes using gene silencing methods and technologies in commercial plant varieties.
目次
1. Recent Advances in Plant Gene Silencing Methods
Prachi Pandey, Kirankumar S. Mysore, and Muthappa Senthil-Kumar
2. Strategies for Efficient RNAi Based Gene Silencing of Viral Genes For Disease Resistance In Plants
Krish K Kumar, Shanmugam Varanavasiappan, Loganathan Arul, Easwaran Kokiladevi, and Duraialagaraja Sudhakar
3. Genome Editing and Designer Crops for the Future
Sumi Rana, Pooja Rani Aggarwal, Varsa Shukla, Urmi Giri, Shubham Verma, and Mehanathan Muthamilarasan
4. In Silico Methods for the identification of Viral-derived Small interfering RNAs (vsiRNAs) and their application in Plant genomics
Aditya Narayan, Shafaque Zahra, Ajeet Singh, and Shailesh Kumar
5. Barley stripe mosaic virus (BSMV)-Based Virus-Induced Gene Silencing to Functionally Characterize Genes In Wheat And Barley
Harvinder Bennypaul and Upinder S. Gill
6. Virus-induced Gene Silencing in Wheat and Related Monocot Species
Vinay Panwar and Kostya Kanyuka
7. Virus-Induced Gene Silencing in Sorghum using Brome mosaic virus
Dharmendra K. Singh and Kirankumar S. Mysore
8. RTBV-based VIGS Vector for Functional Genomics in Rice: Methodology, Advances, Challenges, and Future Implications
Gaurav Kumar, Kamlesh Kumari, and Indranil Dasgupta
9. Optimization of Tobacco rattle virus (TRV)-based Virus-Induced Gene Silencing (VIGS) in Tomato
Ashish Kumar Singh, Dibyendu Ghosh, and Supriya Chakraborty
10. Virus-Induced Gene Silencing for Functional Genomics of Specialized Metabolism in Medicinal Plants
Dikki Pedenla Bomzan, Krishna Kumar, Sarma Rajeev Kumar, Seema Meena, and Dinesh A. Nagegowda
11. VIGS-based Gene Silencing for Assessing Mineral Nutrient Acquisition
Akash, Rajat Srivastava, and Rahul Kumar
12. High throughput Analysis of Gene Function Under Multiple Abiotic Stresses Using Leaf Disks from Silenced Plants
Ramegowda Yamunarani, Venkategowda Ramegowda, Muthappa Senthil-Kumar, and Kirankumar S Mysore
13. A Method for Developing RNAi-derived Resistance in Cowpea Against Geminiviruses
Sanjeev Kumar, Sunil Kumar Mukherjee, Lingaraj Sahoo
14. In vitro method for synthesis of large scale ds RNA molecule as a Novel Plant Protection Strategy
Sundaresha, S, Aarti Bairava, Maharishi Tomar, Ravinder Kumar, E.P. Venkatasalam, Vinay Sagar, Vinay Bhardwaj, and Sanjeev Sharma
15. Fine-tuning Plant Gene Expression with Synthetic Trans-Acting Small Interfering RNAs
Lucio Lopez-Dolz, Maria Spada, Jose-Antonio Daros, and Alberto Carbonell
16. Trans-Kingdom RNA silencing in Plant-Fungal Disease Control
Tao Zhang, Fei Wang, Hui-Shan Guoand Yun Jin
17. An integrated bioinformatics and functional approach for miRNA validation
Sombir Rao, Sonia Balyan, Chandni Bansal, and Saloni Mathur
18. Development of a Ligation-Independent Cloning-Based Dual Vector System for RNA Interference in Plants
Jinping Zhao, Carlos Garcia Rios, Jingjing Xu, Ijaz Ahmad, and Junqi Song
19. Multigene Transformation Through Cre-lox mediated Site-Specific Integration in Rice
Bhuvan Pathak, Soumen Nandy, and Vibha Srivastava
20. An Improvised Hairy Root Transformation Method for Efficient Gene Silencing in Roots and Nodules of Arachis hypogaea
Bikash Raul and Senjuti Sinharoy
21. A method to reduce off-targets in CRISPR/Cas9 system in plants
Ali Movahedi, Zahra Hajiahmadi, Hui Wei, Liming Yang, Honghua Ruan, and Qiang Zhuge
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