Plant secondary metabolism engineering : methods and protocols
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Bibliographic Information
Plant secondary metabolism engineering : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2469）（Springer protocols）
Humana Press, c2022
2nd ed
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Note
Previous ed.: 2010
Includes bibliographical references and index
Description and Table of Contents
Description
This second edition provides detailed practical information on important methods employed in the engineering of plant secondary metabolism pathways. New and updated chapters guide readers through extraction, quantification, purification, localization, characterization, data mining and processing, biosynthesis modulation, and pathway engineering of representative classes of plant specialized metabolites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, list of necessary materials and reagents, tips on troubleshooting and known pitfalls, and step-by-step descriptions of readily reproducible protocols.
Authoritative and cutting-edge, Plant Secondary Metabolism Engineering: Methods and Protocols, Second Edition aims to be a useful practical guide to help researchers working in this exciting field.
Table of Contents
1. Extraction and Analysis of Phenolic Compounds From Grape Berries
Kamal Tyagi, Larry Lerno, Mirko De Rosso, Itay Maoz, Amnon Lichter, Susan E. Ebeler, and Riccardo Flamini
2. Construction of the UDP-glucose biosynthetic Enzyme Gene Co-Expression Plasmid For Prunasin Production in Escherichia coli
Takuya Yamaguchi and Yasuhisa Asano
3. Histochemical Localization of Phenolic Compounds And Reactive Oxygen Species In Eucalypt Microcuttings
Johnatan Vilasboa, Cibele Tesser da Costa, Jorge Ernesto de Araujo Mariath, and Arthur Germano Fett-Neto
4. A Bioinformatics Tool for Efficient Retrieval of High-Confidence Terpene Synthases (TPS) and Application to the Identification of TPS in Coffea and Quillaja
Douglas S. Domingues, Liliane S. Oliveira, Samara M. C. Lemos, Gian C. C. Barros, and Suzana T. Ivamoto-Suzuki
5. Use of Catharanthus roseus Cell Cultures for the Synthesis Of Metal Nanoparticles
Dandy Ahamefula Osibe and Hideki Aoyagi
6. Fungal Pigment Assisted Silver Nanoparticle Synthesis and their Antimicrobial and Cytotoxic Potential
Sharad Bhatnagar, Toshiro Kobori, Deepak Ganesh, and Hideki Aoyagi
7. Nuclei Enrichment from Sugarcane Stems for Proteomics Analyses
Fernanda Salvato, Raquel Figueiredo, and Paulo Mazzafera
8. Visualization of Suberization and Lignification in Sugarcane
Raquel Figueiredo, Juan Pablo Portilla Llerena, Barbara Rocha Cardeli, and Paulo Mazzafera
9. Isolation of Promoters and Transcription Factors Involved In The Regulation of Lignin Biosynthesis in Saccharum species
Juan Pablo Portilla Llerena, Raquel Figueiredo, Savio Siqueira Ferreira, Igor Cesarino, and Paulo Mazzafera
10. Soapbark Triterpenes: Quillaja brasiliensis Cell Culture Sapogenin and Free Sterol Analysis by GCMS
Yve V. S. Magedans and Michael A. Phillips
11. Generation of stable Catharanthus roseus hairy root lines with Agrobacterium rhizogenes
Krystyna K. F. Traverse, Samuel Mortensen, Juliet G. Trautman, Hope Danison, Noreen F. Rizvi, and Carolyn W.T. Lee-Parsons
12. Analyzing the Distribution of Specialized Metabolites From Plant Native Tissues With Laser Desorption Low-Temperature Plasma Mass Spectrometry Imaging Abigail Moreno-Pedraza, Nancy Shyrley Garcia-Rojas and Robert Winkler
13. Virus-Induced Gene Silencing as a Tool to Study Regulation of Alkaloid Biosynthesis in Medicinal Plants
Barunava Patra, Yongliang Liu, Joshua J. Singleton, Sanjay Kumar Singh, and Sitakanta Pattanaik, and Ling Yuan
14. Cyclotide isolation from Psychotria brachyceras and Psychotria leiocarpa
Helio Nitta Matsuura
15. Formulation of IMXQB: nanoparticles based on Quillaja brasiliensis saponins to be used as vaccine adjuvants
Mariana Rivera-Patron, Samuel P. Cibulski, Iris Miraballes, and Fernando Silveira
16. Isolation of specialized plant cells by fluorescence-activated cell sorting
Joana G. Guedes, Ana Lui sa Guimara es, Ine s Carqueijeiro, Rui Gardner, Cla udia Bispo and Mariana Sottomayor
17. X-Ray Computed Tomography for 3D Anatomical Analysis of Resin Ducts
Irenka Fabia n-Plesni kova , Franciele Anto nia Neis, and Adolpho Herbert Augustin
18. Untargeted metabolomic analysis of non-volatile and volatile glucosinolates in Brassicaceae
Yu LIU, Li ZOU, and Choon Nam ONG
19. Methods of mimosine extraction from Leucaena leucocephala (Lam.) de Wit leaves
Kelly Cristine da Silva Rodrigues-Correaa, Michael David Hideo Hondab, Dulal Borthakurb and Arthur G. Fett-Netoa
20. Microbial production, extraction, and quantitative analysis of isoprenoids Alessandro Satta, Zeyu Lu, Manuel R. Plan, Lygie Esquirol, and Birgitta E. Ebert
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