Super-resolution microscopy : methods and protocols
著者
書誌事項
Super-resolution microscopy : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1663）（Springer protocols）
Humana Press, c2017
- : softcover
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注記
Includes bibliographical references and index
"This Humana Press imprint is published by Springer Nature. The registered company is Springer Science+Business Media LLC"--T.p. verso
内容説明・目次
内容説明
This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Super-Resolution Microscopy: Methods and Protocols is a valuable resource for both established and novel researchers and users in this field.
目次
Preface...
Table of Contents...
Contributing Authors...
1) Super-Resolution Microscopy Techniques and their Potential for Applications in Radiation Biophysics
Jan Philipp Eberle, Alexander Rapp, Matthias Krufczik, Marion Eryilmaz, Manuel Gunkel, Holger Erfle, and Michael Hausmann
2) Managing the Introduction of Super-Resolution Microscopy into a Core Facility
Jeffrey A. Kamykowski and Brian Storrie
3) Live-Cell STED Imaging with the HyPer2 Biosensor
Natalia M. Mishina and Vsevolod V. Belousov
4) Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal Microscope
Andre Klauss and Carsten Hille
5) Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In Vivo
Mirelle J.T. ter Veer, Thomas Pfeiffer, and U. Valentin Nagerl
6) STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live Cells
Roman S. Erdmann, Derek Toomre, and Alanna Schepartz
7) Four Channel Super-Resolution Imaging by 3-D Structured Illumination
Ulrike Engel
8) Correlative SIM-STORM Microscopy
O. Burri, T. Laroche, R. Guiet, and A. Seitz
9) Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological Samples
Patricia Bondia, Santiago Casado, and Cristina Flors
10) Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking Cycles
Franziska Fricke, Joel Beaudouin, Sebastian Malkusch, Roland Eils, and Mike Heilemann
11) Two-Color Single-Molecule Tracking in Live Cells
Siegfried Hanselmann and Dirk-Peter Herten
12) Fully Automated Targeted Confocal and Single Molecule Localization Microscopy
Jan Philipp Eberle , Walter Muranyi , Hans-Georg Krausslich , Holger Erfle, and Manuel Gunkel
13) Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution Microscopy
Christopher L. German, Manasa V. Gudheti, Annette E. Fleckenstein, and Erik M. Jorgensen
14) Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured Cells
Errin Johnson and Rainer Kaufmann
15) Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and their use in Cellular Imaging Experiments
Jonathan B. Grimm, Timothy A. Brown, Brian P. English, Timothee Lionnet, and Luke D. Lavis
16) Measuring Nanometer Distances between Fluorescent Labels Step-by-Step
Susanna Maria Fruh and Ingmar Schoen
17) Correlative Single Molecule Localization Microscopy and Confocal Microscopy
Christian Soeller, Yufeng Hou, Isuru D. Jayasinghe, David Baddeley, and David Crossman
18) Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed Cells
Kem A. Sochacki and Justin W. Taraska
19) In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT
Brian J. Beliveau, Alistair N. Boettiger, Guy Nir, Bogdan Bintu, Peng Yin, Xiaowei Zhuang, and C.-ting Wu
20) Super-Resolution High Content Screening and Analysis
K. Soliman
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