Tumor angiogenesis assays : methods and protocols
著者
書誌事項
Tumor angiogenesis assays : methods and protocols
（Methods in molecular biology, 2572）
Springer, 2022
2th ed
- hbk.
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注記
Previous edition: New York: Humana Press, 2016
内容説明・目次
内容説明
This second edition provides new and updated methods and protocols for studying tumor angiogenesis in vitro and in vivo. Chapters detail morphological aspects of tumor angiogenesis, aortic ring, ex vivo tissue culture model for anti-angiogenic drug testing, transgenic zebrafish, orthotopic models of ovarian cancer, and uncovering metabolic effects of anti-angiogenic therapy in tumors by induced metabolic bioluminescence imaging.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Tumor Angiogenesis Assays: Methods and Protocols, Second Edition is a valuable resource for all researchers interested in learning more about this important and developing field.
目次
1. Discovery and development of tumor angiogenesis assays
Gianfranco Natale and Guido Bocci
2. The role of the stroma in tumor angiogenesis and progression: the fundamental contribution of Pietro M. Gullino
Domenico Ribatti
3.Methods for Isolation of Tumor Associated Endothelial Cells for Surface Protein Analysis and Sorting by Flow cytometry
Jelle Verhoeven, Patrizia Agostinis, and Madhur Agrawal
4. Perfusion based fluorescent dye labeling to sort cancer cells based on their distance from blood vessels
Saran Kumar
5. Visualizing the interactions that haping the imaging of microenvironment in cancer diseases
Antonio G. Solimando, Vanessa Desantis, and Matteo Claudio Da Via
6. Quantification of tumor and angiogenic-related markers in ovarian cancer models by a digital pathology approach Ilaria Piga, Sonia Minuzzo, Tiziana Zanin, Giovanni Esposito, and Stefano Indraccolo
7. Monitoring angiotropic extravascular migratory metastasis in vitro
Claire Lugassy, Hynda K. Kleinman, and Raymond L. Barnhill
8. Double immunohistochemical staining on formalin-fixed paraffin-embedded tissue samples to study vascular co-option
Tiziana Annese, Mariella Errede, Michelina De Giorgis, Loredana Lorusso, Roberto Tamma, and Domenico Ribatti
9. Development of Experimental Three-Dimensional Tumor Models to Study Glioblastoma Cancer Stem Cells and Tumor Microenvironment
Henry Ruiz-Garcia, Natanael Zarco, Fumihiro Watanabe, Virginea De Araujo-Farias, Paola Suarez-Meade, Hugo Guerrero-Cazares, Jaime Imitola, Alfredo Quinones-Hinojosa, and Daniel Trifiletti
10. IKOSA (R) CAM Assay application to quantify blood vessels on chick chorioallantoic membrane (CAM)
Tiziana Annese, Roberto Tamma, and Domenico Ribatti
11.Application of laser speckle contrast imaging (LSCI) for the angiogenesis measurement of tumors in the chorioallantoic membrane (CAM) model
Eric Pion, Silke Haerteis, and Thiha Aung
12. The chick CAM as an in vivo system to study stem cell activity
Marta Teixeira Pinto, Ana Sofia Ribeiro, and Joana Paredes
13. Detection of possible symmetries in vascular networks by computer assisted image analysis
Diego Guidolin, Cinzia Tortorella, abd Domenico Ribatti
14. Alternative in vivo models to study teratoma
Michela Corsini and Stefania Mitola
15. How to generate a vascular-labeled transgenic zebrafish model to study tumor angiogenesis and extravasation
Roxana E. Oberkersch, Jacopo Lidonnici, and Massimo M. Santoro
16. 3D human tumor tissues cultured in dynamic conditions as alternative in vitro disease models
Giorgia Pelizzoni and Silvia Scaglione
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