Integrated methods in protein biochemistry

著者

• Shukla, Arun K.

書誌事項

Integrated methods in protein biochemistry

edited by Arun K. Shukla

（Methods in enzymology / editors-in-chief, Anna Marie Pyle, David W. Christianson, v. 675）

Academic Press, an imprint of Elsevier, 2022

1st ed

• Part A

注記

Includes bibliographical references

内容説明・目次

内容説明

Integrated Methods in Protein Biochemistry: Part A, Volume 677, the latest release in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on topics such as DNA and protein engineering to create protein bioswitches with new functions, Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases, An experimental protocol to study lipid transfer proteins, Synthesis of small heat shock proteins, Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins, An experimental protocol for glycoconjugate analysis, Methods for proximity-based biotinylation combined with Mass Spectrometry, and more. Additional chapters cover Synthetic antibody fragments as conformational sensors of protein activation and trafficking, Expression, purification, functional analysis and crystallization of Rag GTPase, Purification of bacterial transcription elongation complexes by photoreversible immobilization, Inhibition of c-Myc-MAX heterodimerization, Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases, and much more.

目次

1. DNA and protein engineering to create protein bioswitches with new functions Stewart Loh 2. Interaction and cross-talk of prelamin A with integral membrane zinc metalloproteases Susan Michaelis 3. An experimental protocol to study lipid transfer proteins Haijia Yu 4. Synthesis of small heat shock proteins Matthew Pratt 5. Druggable p-p interacting sites for Co-chaperone DNAJA1 and its partner proteins Guang-Yu Yang 6. An experimental protocol for glycoconjugate analysis Yifa Zhou 7. Methods for proximity-based biotinylation combined with Mass Spectrometry Ralph Kehlenbach 8. Synthetic antibody fragments as conformational sensors of protein activation and trafficking Arun K. Shukla 9. Expression, purification, functional analysis and crystallization of Rag GTPase Kuang Shen 10. Purification of bacterial transcription elongation complexes by photoreversible immobilization Eric Strobel 11. Inhibition of c-Myc-MAX heterodimerization Neha Garg 12. Fluorogenic RNA aptamers to probe transcription by multi-subunit RNA polymerases Markus C. Wahl 13. Phosphorylation and subcellular localization of human phospholipase A1 Atsushi Yamashita 14. Integrated methods in studying light-sensitive proteins Lars-Oliver Essen 15. Structure and biochemistry of thermophilic protein Ankan Banerjee 16. Using hydrogen/deuterium exchange mass spectrometry to probe protein structure Gert Bange 17. Nanopore force spectroscopy to probe structural dynamics of proteins Sonja Schmid 18. Structural analysis of knotted proteins Shang-Te "Danny" Hsu 19. Mutation-induced structural changes in the SARC-CoV2 spike protein Shang-Te "Danny" Hsu 20. Preparing metabolite capped RNA substrates using RNA polymerases Jeremy G. Bird

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詳細情報

• NII書誌ID(NCID): BC17461629
• ISBN:
  • 9780323992664
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Cambridge, Mass.
• ページ数/冊数: xvii, 437 p.
• 大きさ: 24 cm
• 分類:
  • DC23 : 572.6
• 件名:
  • LCSH : Proteins -- Analysis -- Research -- Methodology
  • LCSH : Proteomics -- Methodology
  • LCSH : Biochemistry
• 親書誌ID:
  • BC02063134