Cancer cell biology : methods and protocols
著者
書誌事項
Cancer cell biology : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2508）（Springer protocols）
Humana Press, c2022
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This volume provides detailed methods on the mechanisms of underlying cancer cell biology. Chapters guide readers through techniques for culturing cancer cell lines, xenografts, cryopreservation of tumor cells, analyzing the co-culture of breast cancer cells, protein secretion by ELISA, flow cytometry-based, multi-parametric immunofluorescence analysis, protein expression by western blot, analysis of surface protein levels, protein recycling by biotinylation assay, and proteomics analysis by liquid chromatography-mass spectrometry. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Cancer Cell Biology: Methods and Protocols aims to provide a
comprehensive set of tools for the analysis of cancer cell biology in the lab.
目次
1. Overview of the Maintenance of Authentic Cancer Cell Lines
Sherri L Christian
2. Culturing Suspension Cancer Cell Lines
Modeline N. Longjohn, Hong-Dien Phan, and Sherri L. Christian
3. Culture of Adherent Cancer Cell Lines
V. Noel and M.D. Berry
4. Breast Cancer Xenograft Murine Models
Wasundara Fernando, Krysta M. Coyle, and Paola Marcato
5. Processing and Cryopreservation of Blood, Cancer Tissues, and Cancer Cells For Viable Biobanking
Jennifer A. Chan and Suzanne M. Vercauteren
6. Adipocyte--Breast Cancer Cell Co--Culture In Transwells
Soofiyah P Ayaani, Martin Wabitsch, and Mark Christian
7. A 3-dimensional Co-Culture Method for Studying Interactions Between Adipocytes, Extracellular Matrix, and Cancer Cells
Emmanuel C. Asante, Nikitha K. Pallegar, Alicia M. Viloria-Petit, and Sherri L. Christian
8. Modelling Collective Invasion and Single-Cell Mesenchymal Invasion in Three-Dimensional Matrigel-Collagen I Cultures
Erin M. Bell, Marcia L. Graves, Pamela M. Dean, Tate O. Goodman, and Calvin D. Roskelley
9. CellTrace (TM) Violet Flow Cytometric Assay to Assess Cell Proliferation
Marina B. Lemieszek, Scott D. Findlay, and Gabrielle M. Siegers
10. Enzyme-Linked Immunosorbent Assay (ELISA)
Mahdis Sadat Tabatabaei and Marya Ahmed
11. Flow Cytometry Analysis to Detect Lapatinib-Induced Modulation of Constitutive and IFN-g induced HLA class I expression in HER2-positive Breast Cancer Cells
Louisa L. Wiede and Sheila Drover
12. An End to End Workflow for Interrogating Tumour Infiltrating Myeloid Cells Using Mass Cytometry
Maunish Barvalia and Kenneth W. Harder
13. Manual Immunofluorescence of Formalin-Fixed Paraffin-Embedded Human Tumour Tissues
Sarah Nersesian and Jeanette E. Boudreau
14. Western Blot Analysis of Lapatinib-Mediated Inhibition of the Epidermal Growth Factor Receptor 2 (HER2) Pathway in Breast Cancer Cells
Louisa L. Wiede and Sheila Drover
15. Evaluating Cell Membrane Localization and Intracellular Transport of Proteins by Biotinylation
Eduardo Reyes-Alvarez, Timothy J. Walker, and Lois M. Mulligan
16. Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags
Mukhayyo Sultonova, Devanand M. Pinto, and J. Patrick Murphy
17. The use of Seahorse XF Assays to Interrogate Real-Time Energy Metabolism in Cancer Cell Lines
Jenna K. Caines, David A. Barnes, and Mark D. Berry
18. Using CRISPR-Cas9 to Dissect Cancer Mutations in Cell Lines
Shady Sayed, Duran Surun, Jovan Mircetic, Olga Sidorova, and Frank Buchholz
19. Single-cell RNA Sequencing Analysis using Fluidigm C1 platform for Characterization of Heterogeneous Transcriptomes
Jinhong Kim and Paola A. Marignani
20. Sequences to Differences in Gene Expression: Analysis of RNA-Seq Data
Polina V. Pavlovich and Pierre Cauchy
21. Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) for Gene Expression Analyses
Albert Caballero-Solares, Jennifer R. Hall, Xi Xue, and Matthew L. Rise
22. Peptide-affinity Isolation of Extracellular Vesicles and cell from Human Plasma -free DNA
Jeremy W. Roy, Catherine A. Taylor, Rodney J. Ouellette, and Stephen M. Lewis
23. Characterizing Extracellular vesicles using Nanoparticle Tracking Analysis
Modeline N. Longjohn and Sherri L. Christian
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