Cancer cell biology : methods and protocols

著者

    • Christian, Sherri L.

書誌事項

Cancer cell biology : methods and protocols

edited by Sherri L. Christian

(Methods in molecular biology / John M. Walker, series editor, 2508)(Springer protocols)

Humana Press, c2022

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume provides detailed methods on the mechanisms of underlying cancer cell biology. Chapters guide readers through techniques for culturing cancer cell lines, xenografts, cryopreservation of tumor cells, analyzing the co-culture of breast cancer cells, protein secretion by ELISA, flow cytometry-based, multi-parametric immunofluorescence analysis, protein expression by western blot, analysis of surface protein levels, protein recycling by biotinylation assay, and proteomics analysis by liquid chromatography-mass spectrometry. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Cancer Cell Biology: Methods and Protocols aims to provide a comprehensive set of tools for the analysis of cancer cell biology in the lab.

目次

1. Overview of the Maintenance of Authentic Cancer Cell Lines Sherri L Christian 2. Culturing Suspension Cancer Cell Lines Modeline N. Longjohn, Hong-Dien Phan, and Sherri L. Christian 3. Culture of Adherent Cancer Cell Lines V. Noel and M.D. Berry 4. Breast Cancer Xenograft Murine Models Wasundara Fernando, Krysta M. Coyle, and Paola Marcato 5. Processing and Cryopreservation of Blood, Cancer Tissues, and Cancer Cells For Viable Biobanking Jennifer A. Chan and Suzanne M. Vercauteren 6. Adipocyte--Breast Cancer Cell Co--Culture In Transwells Soofiyah P Ayaani, Martin Wabitsch, and Mark Christian 7. A 3-dimensional Co-Culture Method for Studying Interactions Between Adipocytes, Extracellular Matrix, and Cancer Cells Emmanuel C. Asante, Nikitha K. Pallegar, Alicia M. Viloria-Petit, and Sherri L. Christian 8. Modelling Collective Invasion and Single-Cell Mesenchymal Invasion in Three-Dimensional Matrigel-Collagen I Cultures Erin M. Bell, Marcia L. Graves, Pamela M. Dean, Tate O. Goodman, and Calvin D. Roskelley 9. CellTrace (TM) Violet Flow Cytometric Assay to Assess Cell Proliferation Marina B. Lemieszek, Scott D. Findlay, and Gabrielle M. Siegers 10. Enzyme-Linked Immunosorbent Assay (ELISA) Mahdis Sadat Tabatabaei and Marya Ahmed 11. Flow Cytometry Analysis to Detect Lapatinib-Induced Modulation of Constitutive and IFN-g induced HLA class I expression in HER2-positive Breast Cancer Cells Louisa L. Wiede and Sheila Drover 12. An End to End Workflow for Interrogating Tumour Infiltrating Myeloid Cells Using Mass Cytometry Maunish Barvalia and Kenneth W. Harder 13. Manual Immunofluorescence of Formalin-Fixed Paraffin-Embedded Human Tumour Tissues Sarah Nersesian and Jeanette E. Boudreau 14. Western Blot Analysis of Lapatinib-Mediated Inhibition of the Epidermal Growth Factor Receptor 2 (HER2) Pathway in Breast Cancer Cells Louisa L. Wiede and Sheila Drover 15. Evaluating Cell Membrane Localization and Intracellular Transport of Proteins by Biotinylation Eduardo Reyes-Alvarez, Timothy J. Walker, and Lois M. Mulligan 16. Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags Mukhayyo Sultonova, Devanand M. Pinto, and J. Patrick Murphy 17. The use of Seahorse XF Assays to Interrogate Real-Time Energy Metabolism in Cancer Cell Lines Jenna K. Caines, David A. Barnes, and Mark D. Berry 18. Using CRISPR-Cas9 to Dissect Cancer Mutations in Cell Lines Shady Sayed, Duran Surun, Jovan Mircetic, Olga Sidorova, and Frank Buchholz 19. Single-cell RNA Sequencing Analysis using Fluidigm C1 platform for Characterization of Heterogeneous Transcriptomes Jinhong Kim and Paola A. Marignani 20. Sequences to Differences in Gene Expression: Analysis of RNA-Seq Data Polina V. Pavlovich and Pierre Cauchy 21. Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) for Gene Expression Analyses Albert Caballero-Solares, Jennifer R. Hall, Xi Xue, and Matthew L. Rise 22. Peptide-affinity Isolation of Extracellular Vesicles and cell from Human Plasma -free DNA Jeremy W. Roy, Catherine A. Taylor, Rodney J. Ouellette, and Stephen M. Lewis 23. Characterizing Extracellular vesicles using Nanoparticle Tracking Analysis Modeline N. Longjohn and Sherri L. Christian

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詳細情報

  • NII書誌ID(NCID)
    BC18501026
  • ISBN
    • 9781071623756
  • 出版国コード
    us
  • タイトル言語コード
    eng
  • 本文言語コード
    eng
  • 出版地
    New York
  • ページ数/冊数
    xi, 379 p.
  • 大きさ
    27 cm
  • 分類
  • 件名
  • 親書誌ID
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