Vectorology for optogenetics and chemogenetics
著者
書誌事項
Vectorology for optogenetics and chemogenetics
(Neuromethods, 195)(Springer protocols)
Humana Press, c2023
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This volume provides an overview of the viral vectors and how they are applied to chemogenetic and optogenetic tools to the study of neural circuits. The chapters in this book are organized into three parts detailing (1) viral vectors, (2) vector modifications and applications, and (3) practical advice for the delivery of viral vectors, verification of injection accuracy, and monitoring of transgene expression. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Authoritative and cutting-edge, Vectorology for Optogenetics and Chemogenetics aims to be a useful practical guide to researches to help further their study in this field.
目次
Part I : Vectors used in systems neuroscience research
Production, testing and verification of Lentivirus for regional targeting in the old-world monkey brain
Walter Lerchner, Alyssa Luz-Ricca, Kiana Dash, Violette DerMinassian and Barry J Richmond
HiRet/NeuRet vectors: Lentiviral system for highly efficient gene transfer through retrograde axonal transport
Kenta Kobayashi, Shigeki Kato, and Kazuto Kobayashi
Generation of High-Titer Defective HSV-1 Amplicon Vectors
Rachael Neve
Generation and Application of Engineered Rabies Viral Vectors for Neural Circuit Research
Masahiro Yamaguchi, Moe Iwata, Riki Kamaguchi, and Fumitaka Osakada
Quality control for Adeno-associated viral vector production
Ina Ersing, Meghan Rego, Chen Wang, Yijun Zhang, Kate Harten DeMaio, Michelle Tillgren, Alanna Mitsopoulos, Gabrielle Clouse, Marcella Patrick, Karen Guerin, and Melina Fan
Part II: Vector modifications and applications
Vector tropism
Shih-Heng Chen, Bo He, Sarwyn Singh, and Negin P. Martin
Viruses for systemic delivery
Zikai Wang, Alon Greenbaum, and Jennifer B. Treweek
Transcriptomic Definition of Neuron Types
Jing He and William R. Stauffer
Enhancers for selective targeting
Jessica Lin and Jordane Dimidschstein
Pathway-selective reversible perturbations using a double-infection technique in the macaque brain
Wim Vanduffel and Tadashi Isa
Pathway-Specific Chemogenetic Manipulation by Applying Ligand to Axonally-Expressed DREADDs
Maricela X Martinez, Mitchell R Farrell, and Stephen V Mahler
Part III: Vector delivery and verification of expression
Convection-enhanced delivery of viral vectors
Devon Griggs, Tiphaine Belloir, Jasmine Zhou, and Azadeh Yazdan-Shahmorad
Multi-channel microinjector arrays for efficient viral vector delivery into rhesus monkey brain
Nanami L. Miyazaki, Bing Li, and Mark A.G. Eldridge
Methods to verify expression and function of DREADDs using PET
Yuji Nagai and Takafumi Minamimoto
Reporter selection and post-mortem methods to verify transgene expression
Kate Heffernan, Yoland Smith and Adriana Galvan
Considerations for the use of viral vectors in NHP experiments
Martin Bohlen and Sebastien Tremblay
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