Molecular plant taxonomy : methods and protocols

書誌事項

Molecular plant taxonomy : methods and protocols

edited by Pascale Besse

(Methods in molecular biology / John M. Walker, series editor, 2222)(Springer protocols)

Humana Press, c2021

2nd ed

  • : [pbk.]

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注記

Includes bibliographical references

内容説明・目次

内容説明

This fully updated edition explores conceptual as well as technical guidelines for plant taxonomists and geneticists, such as the increasing use of next-generation sequencing (NGS) technologies for numerous applications in plant taxonomy. The volume provides molecular approaches to be used within an "integrative taxonomy" framework, combining a range of nucleic acid and cytogenetic data together with other crucial information (taxonomy, morphology, anatomy, ecology, reproductive biology, biogeography, paleobotany, etc.), which will help not only to best circumvent species delimitation but also to resolve the evolutionary processes in play. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Molecular Plant Taxonomy: Methods and Protocols, Second Edition is an ideal guide for researchers seeking a better understanding of evolutionary processes, at species and population level, through molecular techniques.

目次

1. Plant Taxonomy: A Historical Perspective, Current Challenges, and Perspectives Germinal Rouhan and Myriam Gaudeul 2. Guidelines for the Choice of Sequences for Molecular Plant Taxonomy Pascale Besse 3. Isolation and Purification of DNA from Complicated Biological Samples Ruslan Kalendar, Svetlana Boronnikova, and Mervi Seppanen 4. Herbarium Specimens: A Treasure for DNA Extraction, an Update Lenka Zaveska Drabkova 5. Sequencing of Complete Chloroplast Genomes Berthold Heinze 6. Utility of the Mitochondrial Genome in Plant Taxonomic Studies Jerome Duminil and Guillaume Besnard 7. Nuclear Ribosomal RNA Genes: ITS Region Pascale Besse 8. Plant DNA Barcoding Principles and Limits: A Case Study in the Genus Vanilla Pascale Besse, Denis Da Silva, and Michel Grisoni 9. High-Throughput Genotyping Technologies in Plant Taxonomy Monica F. Danilevicz, Cassandria T. Fernandez, Jacob I. Marsh, Philipp E. Bayer, and David Edwards 10. Genotyping-by-Sequencing Technology in Plant Taxonomy and Phylogeny Felicien Favre, Cyril Jourda, Pascale Besse, and Carine Charron 11. Development of Microsatellite Markers Using Next-Generation Sequencing Helene Vignes and Ronan Rivallan 12. Amplified Fragment Length Polymorphism: Applications and Recent Developments Thotten Elampilay Sheeja, Illathidath Payatatti Vijesh Kumar, Ananduchandra Giridhari, Divakaran Minoo, Muliyar Krishna Rajesh, and Kantipudi Nirmal Babu 13. Randomly Amplified Polymorphic DNA (RAPD) and Derived Techniques Kantipudi Nirmal Babu, Thotten Elampilay Sheeja, Divakaran Minoo, Muliyar Krishna Rajesh, Kukkamgai Samsudeen, Erinjery Jose Suraby, and Illathidath Payatatti Vijesh Kumar 14. Inter Simple Sequence Repeats (ISSR), Microsatellite-Primed Genomic Profiling Using Universal Primers Chrissen E.C. Gemmill and Ella R.P. Grierson 15. Retrotransposable Elements: DNA Fingerprinting and the Assessment of Genetic Diversity Ruslan Kalendar, Alexander Muterko, and Svetlana Boronnikova 16. Introduction to Population Genomics Methods Thibault Leroy and Quentin Rougemont 17. The Application of Flow Cytometry for Estimating Genome Size, Ploidy Level Endopolyploidy, and Reproductive Modes in Plants Jaume Pellicer, Robin F. Powell, and Ilia J. Leitch 18. Molecular Cytogenetics (FISH and Fluorochrome Banding): Resolving Species Relationships and Genome Organization Sonja Siljak-Yakovlev, Fatima Pustahija, Vedrana Vicic Bockor, and Odile Robin 19. GISH: Resolving Interspecific and Intergeneric Hybrids Nathalie Piperidis

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