Plant mitochondria : methods and protocols
著者
書誌事項
Plant mitochondria : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2363）（Springer protocols）
Humana Press, c2022
- : [pbk.]
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This detailed volume presents a wide range of techniques for plant mitochondrial analysis, ranging from tried-and-tested work horse techniques to the latest innovations. Within its pages, it explores subjects such as affinity-based isolation of mitochondria with magnetic beads, mitochondrial quality assessment protocols, measurement of uptake and release of specific metabolites, mitochondrial protein identification and visualization, as well as gene splicing and editing, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Mitochondria: Methods and Protocols provides a highly useful set of methodologies for the plant mitochondrial community to help discover more interesting aspects of plant mitochondria in the years to come.
目次
1. Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients
Huy Cuong Tran and Olivier Van Aken
2. Cell Type-Specific Isolation of Mitochondria in Arabidopsis
Clement Boussardon and Olivier Keech
3. Isolation of Plant Mitochondria Using Affinity Purification
Franziska Kuhnert and Andreas P.M. Weber
4. Isolation of Highly Purified, Intact, and Functional Mitochondria from Potato Tubers Using a Two-in-One Percoll Density Gradient
Ian Max Moller and Allan G. Rasmusson
5. Integrity Assessment of Isolated Plant Mitochondria
Allan G. Rasmusson, Mengshu Hao, and Ian Max Moller
6. High-Throughput Oxygen Consumption Measurements in Leaf Tissue Using Oxygen Sensitive Fluorophores
Brendan M. O'Leary, Glenda Guek Khim Oh, and A. Harvey Millar
7. Assessment of Respiratory Enzymes in Intact Cells by Permeabilization with Alamethicin
Allan G. Rasmusson, Ian Max Moller, and Susanne Widell
8. Assessing the Kinetics of Metabolite Uptake and Utilization by Isolated Mitochondria Using Selective Reaction Monitoring-Mass Spectrometry (SRM-MS)
Xuyen Le, A. Harvey Millar, and Chun Pong Lee
9. Complexome Profiling of Plant Mitochondrial Fractions
Lucie Schroeder, Holger Eubel, and Hans-Peter Braun
10. High Throughput BN-PAGE for Mitochondrial Respiratory Complexes
Lilian Vincis Pereira Sanglard and Catherine Colas des Francs-Small
11. Assessment of Mitochondrial Protein Composition and Purity by Mass Spectroscopy
Anqiang Tang, Yimin Wang, and Nicolas L. Taylor
12. Mitochondrial GFP-Tagged Protein Localization Using Transient Transformations in Arabidopsis thaliana
Cristina Ruberti
13. Assessment of Mitochondrial Protein Topology and Membrane Insertion
Kerstin Schafer, Carina Engstler, Korbinian Dischinger, and Chris Carrie
14. Assessment of Protein Synthesis in Mitochondria Isolated from Rosette Leaves and Liquid Culture Seedlings of Arabidopsis
Malgorzata Kwasniak-Owczarek, Artur Tomal, and Hanna Janska
15. Markers for Mitochondrial ROS Status
Monika Ostaszewska-Bugajska, Anna Podgorska, and Bozena Szal
16. Mass Spectrometry-Based Quantitative Cysteine Redox Proteome Profiling of Isolated Mitochondria Using Differential iodoTMT Labeling
Jonas Giese, Jurgen Eirich, Frederik Post, Markus Schwarzlander, and Iris Finkemeier
17. Analysis of the Plant Mitochondrial Transcriptome
Kevin Baudry, Etienne Delannoy, and Catherine Colas des Francs-Small
18. Quantification of Mitochondrial RNA Editing Efficiency Using Sanger Sequencing Data
Mizuki Takenaka
19. Nascent Transcript Sequencing for the Mapping of Promoters in Arabidopsis thaliana Mitochondria
Omar Saleh, Sarlita Dwiani, Julia Rott, and Kristina Kuhn
20. Assessment of Mitochondrial DNA Copy Number, Stability, and Repair in Arabidopsis
Deborah Schatz-Daas, Arnaud Fertet, Frederique Lotfi, and Jose M. Gualberto
21. Measuring the Activity of DNA Repair Enzymes in Isolated Mitochondria
Beatriz Ferrando, Ian Max Moller, and Tinna Stevnsner
22. MitoTALENs: A Method for Targeted Gene Disruption in Plant Mitochondrial Genomes
Shin-ichi Arimura
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