Brain organoid research
著者
書誌事項
Brain organoid research
（Neuromethods, 189）（Springer protocols）
Humana Press, c2023
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This volume explores multiple methods and approaches used to generate human brain and neuroretinal organoids to address fundamental questions in human brain research. The chapters in this book cover topics such as self-organizing brain organoids with ventricles amenable to injection and electroporation; visualization of 3D organoids though the latest advancements in microscopy; generation of 3D retinal tissue with physiologically competent, light-sensitive photoreceptors; modeling brain tumors using genetically edited brain organoids; and brain organoids as a model to study Zika virus and SARS-CoV-2 infections. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Comprehensive and cutting-edge, Brain Organoid Research is a valuable resource for researchers at various levels of learning, ranging from undergraduate students, early researchers, and advanced laboratories. This book aims to be instrumental in moving this developing field forward.
目次
Series Preface...Preface...Table of Contents...Contributing Authors...
1. Self-Organizing Brain Organoids with Ventricles Amenable to Injection and ElectroporationStefano L. Giandomenico and Madeline A. Lancaster
2. Profiling Cell Type-Specific Gene Regulatory Regions in Human Cortical OrganoidsTheresa M. Schutze, Nora Boelicke, Katrin Sameith, and Mareike Albert
3. Visualization of 3D Organoids Through the Latest Advancements in MicroscopyManoj Kumar, Riya Rauthan, Debojyoti Chakraborty, and Senthil Arumugam
4. Generation and Isolation of Retinal Ganglion Cells and Photoreceptors from Human iPSC-Derived Retinal Organoids by Magnetic-Activated Cell SortingGael Orieux, Oriane Rabesandratana, Giuliana Gagliardi, and Olivier Goureau
5. Micromanipulator-Assisted Subretinal Transplantation of Human Photoreceptor Reporter Cell Suspensions into MiceKaren Tessmer, Oliver Borsch, Marius Ader, and Sylvia Gasparini
6. Generation of Three-Dimensional Retinal Tissue with Physiologically Competent, Light Sensitive Photoreceptors from Human Induced Pluripotent Stem CellsSilvia Aparicio-Domingo, Miguel Flores-Bellver, Hannah Cobb, Kang Li, Bogi Conrad, Conan Chen, Joseph A. Brzezinski IV, and M. Valeria Canto-Soler
7. Quantitative Assessment of Fluorescent Reporter Expression in 3D Retinal OrganoidsAnne Vielle, Nathan Mathiyakom, Helen Li, Anna C. Howell, and M. Natalia Vergara
8. A Three-Dimensional Organoid Culture System to Model Invasive Patterns of Patient-Derived Glioma Stem CellsAruljothi Mariappan, Theda Knauth, Roberto Pallini, and Jay Gopalakrishnan
9. Modeling Brain Tumors using Genetically Edited Brain Organoids Linbo Li and Shan Bian
10. Brain Organoids as a Model to Study Zika Virus and SARS-CoV-2 InfectionsVeronica Krenn, Luiza M. Higa, and Patricia P. Garcez
11. Infection of 3D Brain Organoids with Human Pathogenic Viruses Under Biosafety Level 3 Conditions with Subsequent Inactivation to Study Viral Replication, Pathomechanisms, and Other Viral Infection-Mediated EffectsPhilipp Niklas Ostermann, Anand Ramani, Ann Kathrin Bergmann, Ortwin Adams, Jay Gopalakrishnan, and Heiner Schaal
Subject Index List...
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