Basics and methods
Author(s)
Bibliographic Information
Basics and methods
(Textbook of ion channels / edited by Jie Zheng, Matthew C. Trudeau, vol. 1)
CRC Press, 2023
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
Gives newcomers to the field a foundation in ion channel properties
Details up-to-date techniques used in the study of ion channels, including fluorescence spectroscopy, x-ray crystallography, and electrophysiology
Includes case studies and a glossary
Table of Contents
Part I: Fundamental Mechanisms 1. Ion Selectivity and Conductance 2. Voltage-Dependent Gating of Ion Channels 3. Ligand-Dependent Gating Mechanism 4. Mechanosensitive Channels and Their Emerging Gating Mechanisms 5. Inactivation and Desensitization 6. Ion Channel Inhibitors Part II: Methodologies 7. Expression of Channels in Heterologous Systems and Voltage Clamp Recordings of Macroscopic Currents 8. Patch Clamping and Single-Channel Analysis 9. Patch Clamp Recordings from Native Cells and Isolation of Membrane Currents 10. Models of Ion Channel Gating 11. Investigating Ion Channel Structure and Dynamics Using Fluorescence Spectroscopy 12. Ion Channel Structural Biology in the Era of Single Particle Cryo-EM 13. Protein Crystallography 14. Rosetta Structural Modeling 15. Molecular Dynamics 16. Genetic Models and Transgenics 17. EPR and DEER Spectroscopy
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