Gene expression analysis : methods and protocols
Author(s)
Bibliographic Information
Gene expression analysis : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1783）（Springer protocols）
Humana Press, 2018
- : [hardback]
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"Corrected publication 2018"--T.p. verso
Includes bibliographical references and index
Description and Table of Contents
Description
This volume provides experimental and bioinformatics approaches related to different aspects of gene expression analysis. Divided in three sections chapters detail wet-lab protocols, bioinformatics approaches, single-cell gene expression, highly multiplexed amplicon sequencing, multi-omics techniques, and targeted sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Gene Expression Analysis: Methods and Protocols aims provide useful information to researchers worldwide.
Table of Contents
1. Overview of Gene Expression Analysis - Transcriptomics
Nalini Raghavachari and Natalia Reyero
2. RNA-Seq and Expression Arrays: Selection Guidelines for Genome-wide Expression Profiling
Jessica Minnier, Nathan D. Pennock , Quichen Guo, Pepper Schedin, and Christina A. Harrington
3. A Guide for Designing and Analysing RNA-Seq Data
Aniruddha Chatterjee, Antonio Ahn, Euan J Rodger, Peter A Stockwell, and Michael R Eccles
4. SureSelectXT RNA Direct: A Technique for Expression Analysis Through Sequencing of Target-enriched FFPE total RNA
Jennifer Carter Jones, Alex P. Siebold, Carolina Becker Livi, and Anne Bergstrom Lucas
5. Simultaneous, Multiplexed Detection of RNA and Protein on the NanoString (R) nCounter (R) Platform
Sarah Warren
6. Transcript Profiling Using Long-read Sequencing Technologies
Anthony Bayega, Yu Chang Wang, Spyros Oikonomopoulos, Haig Djambazian, Somayyeh Fahiminiya, and Jiannis Ragoussis
7. Making and Sequencing Heavily-Multiplexed, High-throughput 16S Ribosomal RNA Gene Amplicon Libraries Using a Flexible, Two-stage PCR PROTOCOL
Ankur Naqib, Silvana Poggi, Weihua Wang, Marieta Hyde, Kevin Kunstman, and Stefan J. Green
8. microRNA Expression Analysis - Next Generation Sequencing
Poching Liu
9. Identification of Transcriptional Regulators that Bind to Long Noncoding RNAs by RNA Pull Down and RNA Immunoprecipitation
Xiangbo Ruan, Ping Li, and Haiming Cao
10. Single-cell mRNA-Seq using the Fluidigm C1 System and Integrated Fluidics Circuits
Haibiao Gong, Devin Do, and Ramesh Ramakrishnan
11. Current and Future Methods for mRNA Analysis: A Drive Towards Single Molecule Sequencing
Anthony Bayega, Somayyeh Fahiminiya, Spyros Oikonomopoulos, and Jiannis Ragoussis
12. Expression Profiling of Differentially Regulated Genes in Fanconi Anemia
Binita Zipporah E, Kavitha Govarthanan, Pavithra Shyamsunder, and Rama S Verma
13. A Review of Transcriptome Analysis in Pulmonary Vascular Diseases
Dustin R. Fraidenburg and Roberto F. Machado
14. Differential Gene Expression Analysis of Plants
Mark Arick II and Chuan-Yu Hsu
15. High Throughput Sequencing Based Approaches for Gene Expression Analysis.
R. Raja Sekhara Reddy and M. V. Ramanujam
16. Network Analysis of Gene Expression Roby Joehanes
17. Analysis of ChIP-Seq and RNA-Seq data with BioWardrobe
Sushmitha Vallabh, Andrey V. Kartashov, and Artem Barski
18. Bayesian Network to Infer Drug-induced Apoptosis Circuits from Connectivity Map Data
Jiyang Yu and Jose M. Silva
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