Ovarian cancer : methods and protocols
Author(s)
Bibliographic Information
Ovarian cancer : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2424）（Springer protocols）
Humana Press, 2022
- : [hardback]
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"Corrected publication 2022"--T.p. verso
Includes bibliographical references and index
Description and Table of Contents
Description
This detailed volume provides a robust set of methods to understand variation between patients with ovarian cancers, in vitro models to better study different stages of the disease, and in vivo models to test therapies. Beginning with clinical perspectives to orient basic scientists, the book continues with sections exploring methods to characterize features of the tumor microenvironment (TME), techniques to isolate cells for in vitro study, and in vitro and in vivo models to study the disease. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Ovarian Cancer: Methods and Protocols serves as an ideal guide for researchers seeking to improve their study of ovarian cancer and find new therapeutic approaches.
Table of Contents
Part I: Clinical Perspectives
1. Clinical Staging of Ovarian Cancer
Andrea S. O'Shea
2. Pathologic Classification of Ovarian Cancer
Stephanie M. McGregor
Part II: Tumor Microenvironment
3. Multiparameter Flow Cytometry for Detailed Characterization of Peritoneal Immune Cells from Patients with Ovarian Cancer
Jessica Vazquez, Dagna Sheerar, Aleksandar K. Stanic, and Manish S. Patankar
4. Mass Cytometry for the Characterization of Individual Cell Types in Ovarian Solid Tumors
Veronica D. Gonzalez, Ying-Wen Huang, and Wendy J. Fantl
5. Processing and Analysis of Ascites
Hannah M. Micek, Molly J. Carroll, Lisa Barroilhet, and Pamela K. Kreeger
6. Multispectral Staining and Analysis of Extracellular Matrix
Carine M. Renner, Mike R. Visetsouk, Pamela K. Kreeger, and Kristyn S. Masters
7. Quantitative Analysis of the Extracellular Matrix by Immunoblot
Kaitlin C. Fogg
Part III: Cell Isolation
8. Multiparameter Single Cell Characterization of Ovarian Intratumor Heterogeneity
Kristin G. Beaumont, Christina Andreou, Ethan Ellis, and Robert Sebra
9. Culturing Primary Human Mesothelial Cells
Mary Mullen, Hollie Noia, and Katherine Fuh
10. Isolation of Normal and Cancer-Associated Fibroblasts
Katarzyna Zawieracz and Mark A. Eckert
11. Isolation of Primary Normal and Cancer-Associated Adipocytes from the Omentum
Abir Mukherjee
Part IV: Model Systems
12. Isolation of Fallopian Tube Epithelium and Ovaries for Assessment of Cilia Beating Frequency (CBF)
Angela Russo and Joanna E. Burdette
13. Ex Vivo Ovarian Culture to Model the Initial Metastasis in Ovarian Cancer
Matthew Dean
14. In Vivo and Ex Vivo Analysis of Omental Adhesion in Ovarian Cancer
Elizabeth I. Harper and Tyvette S. Hilliard
15. The Role of the Tumor Microenvironment in CSC Enrichment and Chemoresistance: 3D Co-Culture Methods
Michael Bregenzer, Eric Horst, Pooja Mehta, Catherine Snyder, Taylor Repetto, and Geeta Mehta
16. Establishment of In Vivo Ovarian Cancer Mouse Models Using Intraperitoneal Tumor Cell Injection
Sonam Mittal, Prachi Gupta, Pradeep Chaluvally-Raghavan, and Sunila Pradeep
17. Humanized Patient-Derived Xenograft Models of Ovarian Cancer
Sarah B. Gitto, Erin George, Sergey Medvedev, Fiona Simpkins, and Daniel J. Powell, Jr.
18. Xenograft Models of Ovarian Cancer for Therapy Evaluation
Mihaela Popa, Vibeke Fosse, Katrin Kleinmanns, Line Bjorge, and Emmet McCormack
19. Confocal Imaging of Single-Cell Signaling in Orthotopic Models of Ovarian Cancer
Dylan O. Matvey, Thomas S.C. Ng, and Miles A. Miller
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