Stable isotope probing : methods and protocols
著者
書誌事項
Stable isotope probing : methods and protocols
（Basic methods in molecular biology / Leonard G. Davis, Mark D. Dibner, James F. Battey, 2046）
Humana Press : Springer, c2019
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.
目次
1. Experimental Set Up and Data Analysis Considerations for DNA- and RNA-SIP Experiments in the Omics Era
Roey Angel
2. DNA-Based Stable Isotope Probing
Zhongjun Jia, Weiwei Cao, and Marcela Hernandez Garcia
3. RNA Stable Isotope Probing (RNA-SIP)
Noor-Ul-Huda Ghori, Benjamin Moreira-Grez, Paton Vuong, Ian Waite, Tim Morald, Michael Wise, and Andrew S. Whiteley
4. Stable Isotope Probing of Microbial Phospholipid Fatty Acids in Environmental Samples
Andrea Watzinger and Rebecca Hood-Nowotny
5. SIP-Metaproteomics: Linking Microbial Taxonomy, Function, and Activity
Martin Taubert
6. Chip-SIP: Stable Isotope Probing Analyzed with rRNA-Targeted Microarrays and NanoSIMS
Xavier Mayali, Peter K. Weber, Erin Nuccio, Jory Lietard, Mark Somoza, Steven J. Blazewicz, and Jennifer Pett-Ridge
7. Quantification of Methanogenic Pathways Using Stable Carbon Isotopic Signatures
Quan Yuan
8. Stable Isotope-Labeled Single-Cell Raman Spectroscopy Revealing Function and Activity of Environmental Microbes
Li Cui, Kai Yang, and Yong-Guan Zhu
9. Data Analysis for DNA Stable Isotope Probing Experiments Using Multiple Window High Resolution SIP
Samuel E. Barnett, Nicholas D. Youngblut, and Daniel H. Buckley
10. Stable Isotope Probing of Microorganisms in Environmental Samples with H218O
Egbert Schwartz, Michaela Hayer, Bruce A. Hungate, and Rebecca L. Mau
11. Microbial Taxon-Specific Isotope Incorporation with DNA Quantitative Stable Isotope Probing
Brianna K. Finley, Michaela Hayer, Rebecca L. Mau, Alicia M. Purcell, Benjamin J. Koch, Natasja van Gestel, Egbert Schwartz, and Bruce A. Hungate
12. Profiling of Active Microorganisms by Stable Isotope Probing-Metagenomics
Eileen Kroeber and OEzge Eyice
13. Targeted Metatranscriptomics of Soil Microbial Communities with Stable Isotope Probing
Ang Hu, Yahai Lu, Marcela Hernandez Garcia, and Marc G. Dumont
14. Stable Isotope Probing Techniques and Methodological Considerations Using 15N
Roey Angel
15. DNA and RNA Stable Isotope Probing of Methylotrophic Methanogenic Archaea
Xiuran Yin, Ajinkya C. Kulkarni, and Michael W. Friedrich
16. Method Development for DNA and Proteome SIP Analysis of Activated Sludge for Anaerobic Dichloromethane Biodegradation
Miao Hu, Matthew Lee, Ling Zhong, and Michael J. Manefield
17. RNA-Based Stable Isotope Probing (RNA-SIP) in the Gut Environment
Severin Weis, Sylvia Schnell, and Markus Egert
18. Stable Isotope Probing of Microbiota Structure and Function in the Plant Rhizosphere
Wafa Achouak and Feth el Zahar Haichar
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