書誌事項

From mechanisms to disease

edited by José Manuel Bravo-San Pedro, Lorenzo Galluzzi

(Methods in cell biology, v. 175 . Cilia)

Academic Press, an imprint of Elsevier, 2023

  • pt. A

大学図書館所蔵 件 / 3

この図書・雑誌をさがす

注記

Includes bibliographical references

内容説明・目次

内容説明

Cilia: From Mechanisms to Disease, Part A, Volume 175 in the Methods in Cell Biology series, offers a range of techniques and protocols that can be used to study aspects of this interesting cellular organelle both in vitro and in vivo. Sections in this new release include Protocols to induce and study ciliogenesis, Flow Cytometry-Based Approach for the study of primary Cilia, Microscopic observation of human airway ciliary movement using wheat germ agglutinin, Time-lapse imaging of primary cilia behavior with physiological expression of fluorescent ciliary proteins, Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method, and much more. Other sections cover Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish, Visualizing Multiciliated Cells in the Zebrafish, Isolation of Ciliary Ectosomes and Analysis of Peptide-mediated Chemotaxis in Chlamydomonas, Using Paramecium as a Model for Ciliopathies, Using organoids to study cilia, Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model, and more.

目次

1. Protocols to induce and study ciliogenesis Jose Manuel M. Bravo San Pedro 2. A Flow Cytometry-Based Approach for the study of primary Cilia Francesca Ciccolini 3. Microscopic observation of human airway ciliary movement using wheat germ agglutinin Yo Kishimoto 4. Time-lapse imaging of primary cilia behavior with physiological expression of fluorescent ciliary proteins Koji Ikegami 5. Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method Yumiko Saito and Yuki Kobayashi 6. High-efficient CRISPR/Cas9-mediated gene targeting to establish cell models of ciliopathies Tatsuo Miyamoto, Kosuke Hosoba, Tomoka Morita, Ying Zhang, Hiroko Kishi and Takashi Yamamoto 7. Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish Ruxandra Bachman-Gagescu 8. Visualizing Multiciliated Cells in the Zebrafish Rebecca Ann Wingert 9. Isolation of Ciliary Ectosomes and Analysis of Peptide-mediated Chemotaxis in Chlamydomonas Stephen M. King 10. Using Paramecium as a Model for Ciliopathies Judith Van Houten 11. Using organoids to study cilia Vincent GUEN 12. Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model Tang K. Tang, Ting-Yu Chen and Chia-Hsiang Chang

「Nielsen BookData」 より

関連文献: 1件中  1-1を表示

詳細情報

ページトップへ