SiRNA delivery methods : methods and protocols

著者

• Shum, Kato
• Rossi, John J.

書誌事項

SiRNA delivery methods : methods and protocols

edited by Kato Shum and John Rossi

（Methods in molecular biology / John M. Walker, series editor, 1364）（Springer protocols）

Humana Press, c2016

注記

Includes bibliographical references and index

収録内容

• Synthesis and conjugation of small interfering ribonucleic neutral SiRNNs / Alexander S. Hamil and Steven F. Dowdy
• Liver-targeted SiRNA delivery using biodegradable poly(amide) polymer conjugates / Stephanie E. Barrett and Erin N. Guidry
• PepFect6 mediated SiRNA delivery into organotypic cultures / Suvarna Dash-Wagh, Ülo Langel, and Mats Ulfendahl
• Highly efficient SiRNA delivery mediated by cationic helical polypeptides and polypeptide-based nanosystems / Lichen Yin, Nan Zheng, and Jianjun Cheng
• Disulfide-bridged cleavable PEGylation of poly- L -lysine for SiRNA delivery / Min Tang ... [et al.]
• Preparation of a cyclic RGD : modified liposomal SiRNA formulation for use in active targeting to tumor and tumor endothelial cells / Yu Sakurai, Tomoya Hada, and Hideyoshi Harashima
• Multifunctional envelope-type nano device containing a pH-sensitive cationic lipid for efficient delivery of short interfering RNA to hepatocytes in vivo / Yusuke Sato, Hideyoshi Harashima, and Michinori Kohara
• Bioreducible poly (beta-amino ester)s for intracellular delivery of SiRNA / Kristen L. Kozielski and Jordan J. Green
• Preparation of polyion complex micelles using block copolymers for SiRNA delivery / Hyun Jin Kim ... [et al.]
• Delivery of small interfering RNAs to cells via exosomes / Jessica Wahlgren ... [et al.]
• Dendrimer nanovectors for SiRNA delivery / Xiaoxuan Liu and Ling Peng
• Chitosan nanoparticles for SiRNA delivery in vitro / Héloïse Ragelle ... [et al.]
• Non-covalently functionalized of single-walled carbon nanotubes by DSPE-PEG-PEI for SiRNA delivery / King Sun Siu ... [et al.]
• SiRNA in vivo-targeted delivery to murine dendritic cells by oral administration of recombinant yeast / Kun Xu ... [et al.]
• TLR9-targeted SiRNA delivery in vivo / Dewan Md Sakib Hossain ... [et al.]
• Aptamer-MiRNA conjugates for cancer cell-targeted delivery / Carla L. Esposito, Silvia Catuogno, and Vittorio de Franciscis
• Method for confirming cytoplasmic delivery of RNA aptamers / David D. Dickey ... [et al.]
• Hapten-binding bispecific antibodies for the targeted delivery of SiRNA and SiRNA-containing nanoparticles / Irmgard S. Thorey ... [et al.]
• Stable delivery of CCR5-directed shRNA into human primary peripheral blood mononuclear cells and hematopoietic stem/progenitor cells via a lentiviral vector / Saki Shimizu, Swati Seth Yadav, and Dong Sung An
• Hepatic delivery of artificial micro RNAs using helper- dependent adenoviral vectors / Carol Crowther, Betty Mowa, and Patrick Arbuthnot
• Intravascular AAV9 administration for delivering RNA silencing constructs to the CNS and periphery / Brett D. Dufour and Jodi L. McBride
• Efficient gene suppression in dorsal root ganglia and spinal cord using adeno-associated virus vectors encoding short-hairpin RNA / Mitsuhiro Enomoto ... [et al.]
• Synthetic SiRNA delivery : progress and prospects / Thomas C. Roberts ... [et al.]

内容説明・目次

内容説明

This volume provides a collection of cutting-edge strategies in siRNA delivery that were developed and refined over the years with tried-and-true methods. Written by a team of internationally renowned authors, this book describes, in detail, a variety of successful siRNA delivery methods, including peptide-based nanoparticles, liposomes, exosomes, polymers, aptamers, and viral vehicles. Written in the highly successful Methods in Molecular Biology series format, each proven protocol includes brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, SiRNA Delivery Methods: Methods and Protocols, will provide researchers, educators, clinicians, and biotechnology specialists with a broad understanding of the issues in siRNA delivery and how they can be overcome strategically.

目次

1. Synthesis and Conjugation of Small Interfering RiboNucleic Neutral siRNNs Alexander S. Hamil and Steven F. Dowdy 2. Liver-Targeted siRNA Delivery Using Biodegradable Poly(amide) Polymer Conjugates Stephanie E. Barrett and Erin N. Guidry 3. PepFect6 Mediated siRNA Delivery into Organotypic Cultures Suvarna Dash-Wagh, UElo Langel, and Mats Ulfendahl 4. Highly Efficient siRNA Delivery Mediated by Cationic Helical Polypeptides and Polypeptide-Based Nanosystems Lichen Yin, Nan Zheng, and Jianjun Cheng 5. Disulfide-Bridged Cleavable PEGylation of Poly-L-Lysine for siRNA Delivery Min Tang, Haiqing Dong, Xiaojun Cai, Haiyan Zhu, Tianbin Ren, and Yongyong Li 6. Preparation of a Cyclic RGD: Modified Liposomal siRNA Formulation for Use in Active Targeting to Tumor and Tumor Endothelial Cells Yu Sakurai, Tomoya Hada, and Hideyoshi Harashima 7. A Multifunctional Envelope-Type Nano Device Containing A pH-Sensitive Cationic Lipid for Efficient Delivery of Short Interfering RNA to Hepatocytes In Vivo Yusuke Sato, Hideyoshi Harashima, and Michinori Kohara 8. Bioreducible Poly(Beta-Amino Ester)s for Intracellular Delivery of siRNA Kristen L. Kozielski and Jordan J. Green 9. Preparation of Polyion Complex Micelles Using Block Copolymers for siRNA Delivery Hyun Jin Kim, Meng Zheng, Kanjiro Miyata, and Kazunori Kataoka 10. Delivery of Small Interfering RNAs to Cells via Exosomes Jessica Wahlgren, Luisa Statello, Gabriel Skogberg, Esbjoern Telemo, and Hadi Valadi 11. Dendrimer Nanovectors for siRNA Delivery Xiaoxuan Liu and Ling Peng 12. Chitosan Nanoparticles for siRNA Delivery In Vitro Heloise Ragelle, Kevin Vanvarenberg, Gaelle Vandermeulen, and Veronique Preat 13. Non-Covalently Functionalized of Single-Walled Carbon Nanotubes by DSPE-PEG-PEI for siRNA Delivery King Sun Siu, James Koropatnick, and Wei-Ping Min 14. siRNA In Vivo Targeted Delivery to Murine Dendritic Cells by Oral Administration of Recombinant Yeast Kun Xu, Zhongtian Liu, Long Zhang, Tingting Zhang, and Zhiying Zhang 15. TLR9 Targeted siRNA Delivery In Vivo Dewan Md Sakib Hossain, Dayson Moreira, Qifang Zhang, Sergey Nechaev, Piotr Swiderski, and Marcin Kortylewski 16. Aptamer-miRNA Conjugates for Cancer Cells Targeted Delivery Carla L. Esposito, Silvia Catuogno, and Vittorio de Franciscis 17. Method for Confirming Cytoplasmic Delivery of RNA Aptamers David D. Dickey, Justin P. Dassie, and Paloma H. Giangrande 18. Hapten-Binding Bispecific Antibodies for Targeted Delivery of siRNA and siRNA-Containing Nanoparticles Irmgard S. Thorey, Michael Grote, Klaus Mayer, and Ulrich Brinkmann 19. Stable Delivery of CCR5 Directed shRNA into Human Primary Peripheral Blood Mononuclear Cells and Hematopoietic Stem/Progenitor Cells via a Lentiviral Vector Saki Shimizu, Swati Seth Yadav, and Dong Sung An 20. Hepatic Delivery of Artificial Micro RNAs Using Helper-Dependent Adenoviral Vectors Carol Crowther, Betty Mowa, and Patrick Arbuthnot 21. Intravascular AAV9 Administration for Delivering RNA Silencing Constructs to the CNS and Periphery Brett D. Dufour and Jodi L. McBride 22. Efficient Gene Suppression in Dorsal Root Ganglia (DRG) and Spinal Cord Using Adeno-Associated Virus (AAV) Vector Encoding Short-Hairpin RNA Mitsuhiro Enomoto, Takashi Hirai, Hidetoshi Kaburagi, and Takanori Yokota 23. Synthetic siRNA Delivery: Progress and Prospects Thomas C. Roberts, Kariem Ezzat, Samir EL Andaloussi, and Marc S. Weinberg

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詳細情報

• NII書誌ID(NCID): BD0183273X
• ISBN:
  • 9781493931118
• LCCN: 2015947262
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xiii, 313 p.
• 大きさ: 26 cm
• 分類:
  • LCC : QP623.5.S63
  • NLM : W1
  • NLM : QU 25
  • DC23 : 572.8/8
• 件名:
  • LCSH : Small interfering RNA -- Laboratory manuals
  • LCSH : Medicine
  • LCSH : Human genetics
  • MESH : RNA, Small Interfering
  • MESH : Laboratory Manuals
• 親書誌ID:
  • BA10794673
  • BA88873632