Prions : methods and protocols
著者
書誌事項
Prions : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 1658)(Springer protocols)
Humana Press, c2017
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注記
"Softcover reprint of the hardcover 1st edition 2017"--T.p. verso
Includes bibliographical references and index
内容説明・目次
内容説明
This volume details protocols on prion disease from multiple disciplines and highlights the contribution each discipline has made to the understanding of prion disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Prions: Methods and Protocols aims to ensure successful results in the further study of this vital field.
目次
PART I: Methods for Biochemical and Biophysical Analysis of Prion Disease
1. Purification and Fibrillation of Full-length Recombinant PrP Natallia Makarava, Regina Savtchenko, and Ilia V. Baskakov
2. Method for Folding of Recombinant Prion Protein to Soluble b-sheet Secondary Structure
Laura J. Ellett
3. Analysis of Prion Protein Conformation using Circular Dichroism Spectroscopy
Laura J Ellett and Vanessa Johanssen
4. Analysis of Prion Protein Structure using Nuclear Magnetic Resonance Spectroscopy
Ivana Biljan, Gregor Ilc, and Janez Plavec
5. Immunodetection of PrPSc using Western Immunoblotting Techniques
Gerald S. Baron and Gregory J. Raymond
6. Analysis of miRNA Signatures in Neurodegenerative Prion Disease
Shayne A. Bellingham and Andrew F. Hill
PART II: Methods for Cellular Analysis Of Prion Disease
7. Cell Biology Approaches to Studying Prion Diseases
Suzette A. Priola
8. Expression of Heterologous PrP and Prion Propagation in RK13 Cells
Zaira E. Arellano-Anaya, Alvina Huor, Pascal Leblanc, Olivier Andreoletti, and Didier Vilette
9. Generation of Infectious Prions and Detection with the Prion Infected Cell Assay
Laura J Vella, Bradley Coleman, and Andrew F Hill
10. Analysis of Cellular Prion Protein Endoproteolytic Processing
Victoria Lewis
11. Cellular Analysis Adult Neural Stem Cells for Investigating Prion Biology
Cathryn L Haigh
12. Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons
Giuseppe D. Ciccotosto, Metta Jana, and Roberto Cappai
PART III: Methods for Cell Free Propagation of Prions
13. Methods of Protein Misfolding Cyclic Amplification Natallia Makarava, Regina Savtchenko, and Ilia V. Baskakov
14. RT-QuIC Assays for Prion Disease Detection and Diagnostics
Christina D. Orru, Bradley R. Groveman, Andrew G. Hughson, Matteo Manca, Lynne D. Raymond, Gregory J. Raymond, Katrina J. Campbell, Kelsie J. Anson, Allison Kraus, and Byron Caughey
15. A Quick Method to Evaluate the Effect of the Amino Acid Sequence in the Misfolding Proneness of the Prion Protein
Natalia Fernandez-Borges, Hasier Erana, Saioa Rodriguez-Elezgarai, Chafik Harrathi, Vanesa Venegas, and Joaquin Castilla
PART IV: In vivo Investigation of Prion Disease
16. Insights into Mechanisms of Transmission and Pathogenesis from Transgenic Mouse Models of Prion Diseases
Julie A. Moreno and Glenn C. Telling
17. In vivo Near Infrared Imaging of Neurodegeneration
Victoria A Lawson, Carolin Tumpach, Cathryn L Haigh, and Simon C Drew
18. Strain Typing of Prion Diseases using in vivo Mouse Models
Aileen Boyle, Kris Hogan, Jean C. Manson, and Abigail B Diack
19. Preparation and Immunostaining of the Myenteric Plexus of Prion Infected Mice
Laura J. Ellett and Victoria A. Lawson
PART V: Methods for the Detection and Treatment of Prion Diseases
20. Cell Culture Methods for Screening of Prion Therapeutics
Hilary E. McMahon
21. Real-Time Quaking Induced Conversion for Diagnosis of Prion Disease
Katsuya Satoh, Ryuichiro Atarashi, and Noriyuki Nishida
22. Methods for Molecular Diagnosis of Human Prion Disease
Jonathan D. F. Wadsworth, Gary Adamson, Susan Joiner, Lara Brock, Caroline Powell, Jacqueline M. Linehan, Jonathan A. Beck, Sebastian Brandner, Simon Mead, and John Collinge
23. Molecular sub-typing of PrPres in Human Sporadic CJD Brain Tissue
Klug, G.M., Lewis, V., and Collins, S.J.
Part VI: Methods for Investigation of Prion-like Disease
24. Intercellular Prion-like Conversion and Transmission of Cu/Zn Superoxide Dismutase (SOD1) in Cell Culture
Leslie I. Grad, Edward Pokrishevsky, and Neil R. Cashman
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