Single domain antibodies : methods and protocols

著者

• Saerens, Dirk
• Muyldermans, Serge

書誌事項

Single domain antibodies : methods and protocols

edited by Dirk Saerens and Serge Muyldermans

（Methods in molecular biology / John M. Walker, series editor, 911）（Springer protocols）

Humana Press, c2012

内容説明・目次

内容説明

The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications. In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

目次

Part I: Overview of Single Domain Antibodies 1. From Whole Monoclonal Antibodies to Single Domain Antibodies: Think Small Jean-Luc Teillaud 2. Introduction to Heavy Chain Antibodies and Derived Nanobodies Cecile Vincke and Serge Muyldermans 3. Overview and Discovery of IgNARs and Generation of VNARs Stewart D. Nuttall Part II: Single Domain Antibody Library Construction 4. Creation of the Large and Highly Functional Synthetic Repertoire of Human VH and V Domain Antibodies Olga Ignatovich, Laurent Jespers, Ian M, Tomlinson, and Ruud M.T. de Wildt 5. Preparation of a Naive Library of Camelid Single Domain Antibodies Aurelien Olichon and Ario de Marco Part III: Selection of Single Domain Antibodies 6. Selection by Phage Display of Single Domain Antibodies Specific to Antigens in Their Native Conformation Peter Verheesen and Toon Laeremans 7. Semi-Automated Panning of Naive Camelidae Libraries and Selection of Single-Domain Antibodies Against Peptide Antigens Jyothi Kumaran, Roger MacKenzie, and Mehdi Arbabi-Ghahroudi 8. Pichia Surface Display: A Tool for Screening Single Domain Antibodies Kristof De Schutter and Nico Callewaert 9. Bacterial Two Hybrid: A Versatile One-Step Intracellular Selection Method Mireille Pellis, Serge Muyldermans, and Cecile Vincke 10. Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies Tomoyuki Tanaka and Terence H. Rabbitts 11. Selection of Functional Single Domain Antibody Fragments for Interfering with Protein-Protein Interactions Inside Cells: A "One Plasmid" Mammalian Two-Hybrid System Tomoyuki Tanaka and Terence H. Rabbitts 12. Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization Armin Sepp and Andrew Griffiths 13. Selection of VHHs Under Application Conditions Edward Dolk, Theo Verrips, and Hans de Haard 14. Isolation and Characterization of Clostridium difficile Toxin-Specific Single-Domain Antibodies Greg Hussack, Mehdi Arbabi-Ghahroudi, C. Roger MacKenzie, and Jamshid Tanha 15. Selection of VHH Antibody Fragments that Recognize Different A Depositions Using Complex Immune Libraries Rinse Klooster, Kim S. Rutger, and Sylvere van der Maarel Part IV: Expression of Single Domain Antibodies and Derivatives 16. Expression of Single-Domain Antibodies in Bacterial Systems Toya Nath Baral and Mehdi Arbabi-Ghahroudi 17. Expression of VHHs in S. cerevisiae Andrea Gorlani, Hans de Haard, and Theo Verrips 18. Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells Vishal Agrawal, Igor Slivac, Sylvie Perret, Louis Bisson, Gilles St-Laurent, Yanal Murad, Jianbing Zhang, and Yves Durocher 19. Production of Camel-Like Antibodies in Plants Sylvie De Buck, Vikram Virdi, Thomas De Meyer, Kirsten De Wilde, Robin Piron, Jonah Nolf, Els Van Lerberge, Annelies De Paepe, and Ann Depicker Part V: Improvement and Applications of Single Domain Antibodies 20. Selecting and Purifying Autonomous Human Variable Heavy (VH) Domains Raffi Tonikian and Sachdev S. Sidhu 21. Solubility and Stability Engineering of Human VH Domains Dae Young Kim, Wen Ding, and Jamshid Tanha 22. Improvement of Proteolytic Stability through In Silico Engineering Lucy Rutten, Hans de Haard, and Theo Verrips 23. Selection of Human VH Single Domains with Improved Biophysical Properties by Phage Display Kip Dudgeon, Romain Rouet, Kristoffer Famm, and Daniel Christ 24. Improvement of Single Domain Antibody Stability by Disulfide Bond Introduction Yoshihisa Hagihara and Dirk Saerens 25. Characterization of Single-Domain Antibodies with an Engineered Disulfide Bond Greg Hussack, C. Roger MacKenzie, and Jamshid Tanha 26. Affinity Maturation of Single-Domain Antibodies by Yeast Surface Display Akiko Koide and Shohei Koide 27. Multivalent Display of Single-Domain Antibodies Jianbing Zhang and C. Roger MacKenzie 28. Methods for Determining the PK Parameters of AlbudAbs and of Long Serum Half Life Drugs Made Using the AlbudAb Technology Daniel Rycroft and Lucy J. Holt 29. Fluorescent Protein Specific Nanotraps to Study Protein-Protein Interactions and Histone-Tail Peptide Binding Garwin Pichler, Heinrich Leonhardt, and Ulrich Rothbauer 30. Site-Specific Labeling of His-Tagged Nanobodies with 99mTc: A Practical Guide Catarina Xavier, Nick Devoogdt, Sophie Hernot, Ilse Vaneycken, Matthias D'Huyvetter, Jens De Vos, Sam Massa, Tony Lahoutte, and Vicky Caveliers 31. Nanobody-Based Chromatin Immunoprecipitation Trong Nguyen Duc, Gholamreza Hassanzadeh-Ghassabeh, Dirk Saerens, Eveline Peeters, Daniel Charlier, and Serge Muyldermans 32. User-Friendly Expression Plasmids Enable the Fusion of VHHs to Application-Specific Tags Ario de Marco 33. Application of Single Domain Antibodies in Tumor Histochemistry Kien T. Mai and C. Roger MacKenzie Part VI: Case Studies 34. Nanobodies as Structural Probes of Protein Misfolding and Fibril Formation Erwin De Genst and Christopher M. Dobson 35. Molecular Imaging Using Nanobodies: A Case Study Nick Devoogdt, Catarina Xavier, Sophie Hernot, Ilse Vaneycken, Matthias D'Huyvetter, Jens De Vos, Sam Massa, Patrick De Baetselier, Vicky Caveliers, and Tony Lahoutte 36. Case Study on Live Cell Apoptosis-Assay Using Lamin-Chromobody Cell-Lines for High-Content Analysis Kourosh Zolghadr, Jacqueline Gregor, Heinrich Leonhardt, and Ulrich Rothbauer Stewart D. Nuttall

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詳細情報

• NII書誌ID(NCID): BD02164671
• ISBN:
  • 9781617799679
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xv, 580 p.
• 大きさ: 27 cm
• 分類:
  • DC23 : 616.0798
• 件名:
  • LCSH : Immunoglobulins
• 親書誌ID:
  • BA10794673
  • BA88873632