Modifications and targeting of protein termini
Author(s)
Bibliographic Information
Modifications and targeting of protein termini
（Methods in enzymology / editors-in-chief, Anna Marie Pyle, David W. Christianson, v. 684）
Academic Press, an imprint of Elsevier, 2023
1st ed
- pt. A
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Note
Includes bibliographical references
Description and Table of Contents
Description
Modifications and Targeting of Protein Termini, Volume 684 in the Methods in Enzymology series serial highlights new advances in the field, with this new volume presenting interesting chapters pn a variety of timely topics, including Optimizing purification and activity assays of N-terminal methyltransferase complexes, In vitro reconstitution of hierarchical steps of Arg/N-degron pathway, Identification of N-degrons and N-recognins by using peptide-pull-downs combined with quantitative proteomics, A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods, and more.
Additional sections cover Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway, Characterization and chemical modulation of p62/SQSTM1 as an autophagic N-recognin of the Arg/N-degron pathway, Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae, and so much more.
Table of Contents
1. Selective ribosome profiling as a tool to study interactions of translating ribosomes in mammalian cells Bernd Bukau, Jiri Koubek, Manuel Gunnigmann and Gunter Kramer 2. Deformylation of nascent chains on the ribosome Marina V. Rodnina, Lena Boegeholz, Evan Mercier and Wolfgang Wintermeyer 3. Optimizing purification and activity assays of N-terminal methyltransferase complexes Christine Schaner Tooley, Haley Parker and John Tooley 4. Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methods Rong Huang and Ying Meng 5. Biochemical and structural analysis of N-myristoyltransferase-mediated protein tagging Thierry Meinnel, Carmela Giglione, Paul Monassa, Frederic Riviere, Cyril Dian and Frederic Frottin 6. Kinetic and catalytic features of N-myristoyltransferases Thierry Meinnel and Carmela Giglione 7. Detecting N-terminal myristoylation using metabolic Alk12 labeling Hening Lin and Yilai Xu 8. Peptide CoA conjugates for in situ proteomics profiling of acetyltransferase activities Iris Finkemeier, Jurgen Eirich, Julia Sindlinger, Stefan Schoen and Dirk Schwarzer 9. A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms Kris Gevaert, Annelies Bogaert, Tessa Van de Steene, Marnik Vuylsteke and Sven Eyckerman 10. The amino-dipeptidyl peptidases DPP8 and DPP9: purification, interaction studies and enzymatic assays Ruth Geiss-Friedlander, Laura Donzelli and Oguz Bolgi
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