Magnaporthe oryzae : methods and protocols

著者

    • Jacob, Stefan

書誌事項

Magnaporthe oryzae : methods and protocols

edited by Stefan Jacob

(Methods in molecular biology / John M. Walker, series editor, 2356)(Springer protocols)

Humana Press, c2021

  • : pbk

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume highlights molecular methods to study the phytopathogenic rice blast fungus Magnaporthe oryzae. Chapters in this book cover the history, development, and evolution of the pathogen; molecular methods to increase the knowledge of the biology, genetic, and metabolic diversity of the pathogen; and the pathogen's adaptability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Magnaporthe oryzae: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this developing field.

目次

Preface... Table of Contents... Contributing Authors... 1. The Impact of Blast Disease: Past, Present, and Future Barbara Valent 2. The Biology of Invasive Growth by the Rice Blast Fungus Magnaporthe oryzae Neftaly Cruz-Mireles, Iris Eisermann, Marisela Garduno-Rosales, Camilla Molinari, Lauren S. Ryder, Bozeng Tang, Xia Yan, and Nicholas J. Talbot 3. Analysis of the Secondary Metabolism in Magnaporthe oryzae Elizabeth Skellam 4. The Determination of Carbohydrates by High-Performance Anion-Exchange Chromatography Coupled with Pulsed Amperometric Detection (HPAEC-PAD) Christiane Grunewald, Stefan Bohnert, and Stefan Jacob 5. Characterizing the Secretion Systems of Magnaporthe oryzae Ely Oliveira-Garcia and Barbara Valent 6. Specimen Preparation and Observations of Magnaporthe oryzae Appressorial Cells Under Electron Microscopy Raquel O. Rocha and Richard A. Wilson 7. 4D Widefield Fluorescence Imaging of Appressorium Morphogenesis by Magnaporthe oryzae Audra Rogers, Nawaraj Dulal, and Martin Egan 8. Tandem Affinity Purification (TAP) of Low Abundance Protein Complexes in Filamentous Fungi Demonstrated using Magnaporthe oryzae Gang Li and Richard A. Wilson 9. Quantitative Proteome and Phosphoproteome Profiling in Magnaporthe oryzae Thomas Michna and Stefan Tenzer 10. A Target-Based In Vivo Test System to Identify Novel Fungicides with Mode of Action in the HOG Pathway Stefan Bohnert, Hendrik Neumann, and Stefan Jacob 11. Making Visualization Work for You: Deriving Valuable Insights from Omics Data Alexander Yemelin 12. Genome Editing using CRISPR/Cas9 System in the Rice Blast Fungus Takayuki Arazoe 13. Diurnal, Circadian, and Photomorphogenic Analyses in Magnaporthe oryzae Ciaran Griffin and George R. Littlejohn 14. Methods to Study Autophagocytosis in Magnaporthe oryzae Lin Li, Xue-Ming Zhu, Xiao-Hong Liu, and Fu-Cheng Lin 15. Molecular Detection of Magnaporthe oryzae from Rice Seeds Sonia Chadha 16. Isolation and Functional Analysis of Effector-Proteins of Magnaporthe oryzae Meng-Di Dai, Yu Li, Li-Xiao Sun, Fu-Cheng Lin, and Xiao-Hong Liu 17. Analysis of Genetic Variations and Genomic Instabilities in Magnaporthe oryzae Sonia Chadha 18 Controllable Bypass Suppression in Magnaporthe oryzae Stefan Jacob and Katharina Bersching Subject Index List...

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詳細情報

  • NII書誌ID(NCID)
    BD02890006
  • ISBN
    • 9781071616154
  • 出版国コード
    us
  • タイトル言語コード
    eng
  • 本文言語コード
    eng
  • 出版地
    New York
  • ページ数/冊数
    x, 234 p.
  • 大きさ
    26 cm
  • 分類
  • 件名
  • 親書誌ID
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