The immunological synapse

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Bibliographic Information

The immunological synapse

edited by Clément Thomas, Lorenzo Galluzzi

(Methods in cell biology, v. 178)

Academic Press, an imprint of Elsevier, 2023

  • pt. B

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Includes bibliographical references

Description and Table of Contents

Description

The Immunological Synapse - Part B, Volume 178 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. This first volume covers various aspects on T cell and natural killer (NK) cell synapses, including imaging polarized granule release using TIRF microscopy, analysis of actin reorganization and centrosome polarization, redirected degranulation, live cell-imaging to quantify cytotoxic and chemotactic dynamics, quantification of interactions between APCs and T cells, assessment of membrane lipid state at the immunological synapse, proteomic analysis and imaging of NK-tumor cell interaction, evaluating natural killer cell effector functions against breast cancer cells derived from human tumor tissue, evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayer, and monitoring potency of therapeutic CAR T cells. It also includes one chapter on quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, and one chapter on the murine antibody-dependent cellular phagocytosis assay.

Table of Contents

Contributors in this volume include: Torsten Tonn Jiri Eitler Rose Thierry Clement Thomas Jacques Zimmer Carsten Watzl Pedro Proda Arnika Kathleen Wagner Alicia Copik Claudio Giraudo Loic Dupre Felix Borgmann Chenguang Xu Wanli Liu

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