Organ regeneration : 3D stem cell culture & manipulation

著者

書誌事項

Organ regeneration : 3D stem cell culture & manipulation

edited by Takashi Tsuji

（Methods in molecular biology / John M. Walker, series editor, 1597）

Springer Science+Business Media, 2017

タイトル別名

3D stem cell culture and manipulation

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume provides readers with a better understanding of organogenesis in developmental biology and next-generation organ regenerative therapy. This book focuses on recent studies of organ regeneration from stem cells using in vitro 3D cell culture and manipulation. The chapters cover topics such as generation of a 3D retinal tissue formation; functional salivary gland regeneration; liver regeneration using cultures liver bud; and in vivo model of small intestine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Organ Regeneration: 3D Stem Cell Culture and Manipulation is a valuable resource for scientists and researchers who are interested in this field.

目次

Dedication... Preface... Table of Contents... Contributing Authors... 1 Generation of Various Telencephalic Regions from Human Embryonic Stem Cells in Three-Dimensional Culture Taisuke Kadoshima, Hideya Sakaguch, and Mototsugu Eiraku 2 Generation of a Three-Dimensional Retinal Tissue from Self-Organizing Human ESC Culture Atsushi Kuwahara, Tokushige Nakano, and Mototsugu Eiraku 3 3D Culture for Self-Formation of the Cerebellum from Human Pluripotent Stem Cells through Induction of the Isthmic Organizer Keiko Muguruma 4 Reconstitution of a Patterned Neural Tube from Single Mouse Embryonic Stem Cells Keisuke Ishihara, Adrian Ranga, Matthias P. Lutolf, Elly M. Tanaka, and Andrea Meinhardt 5 Functional Pituitary Tissue Formation Chikafumi Ozone and Hidetaka Suga 6 Directed Differentiation of Mouse Embryonic Stem Cells into Inner Ear Sensory Epithelia in 3D Culture Jing Nie, Karl R. Koehler, and Eri Hashino 7 Generation of Functional Thyroid Tissue using 3D-Based Culture of Embryonic Stem Cells Francesco Antonica, Dominika Figini Kasprzyk, Andrea Alex Schiavo, Mirian Romitti, and Sabine Costagliola 8 Functional Tooth Regeneration Masamitsu Oshima, Miho Ogawa, and Takashi Tsuji 9 Functional Hair Follicle Regeneration by the Rearrangement of Stem Cells Kyosuke Asakawa, Koh-ei Toyoshima, and Takashi Tsuji 10 Functional Salivary Gland Regeneration Miho Ogawa and Takashi Tsuji 11 Generation of a Bioengineered Lacrimal Gland by using the Organ Germ Method Masatoshi Hirayama and Takashi Tsuji 12 Generation of Gastrointestinal Organoids from Human Pluripotent Stem Cells Jorge O. Munera and James M. Wells 13 Generation of a Three-Dimensional Kidney Structure from Pluripotent Stem Cells Yasuhiro Yoshimura, Atsuhiro Taguchi, and Ryuichi Nishinakamura 14 Making a Kidney Organoid using the Directed Differentiation of Human Pluripotent Stem Cells Minoru Takasato and Melissa H. Little 15 Liver Regeneration using Cultured Liver Bud Keisuke Sekine, Takanori Takebe, and Hideki Taniguchi 16 Formation of Stomach Tissue by Organoid Culture using Mouse Embryonic Stem Cells Taka-aki K. Noguchi and Akira Kurisaki 17 In Vivo Model of Small Intestine Mahe M. Maxime, Nicole E. Brown, Holly M. Poling, and Helmrath A. Michael

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