Celiac disease
Author(s)
Bibliographic Information
Celiac disease
（Methods in cell biology, v. 179）
Academic Press, an imprint of Elsevier, 2023
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Note
Includes bibliographical references
Description and Table of Contents
Description
Celiac Disease, Volume 179 in the Methods in Cell Biology series is composed of extensive protocols about novel and widely used techniques for celiac disease research. Topics covered in this volume include Pepsin trypsin digested gliadin treatment of intestinal cells in vitro, Ex vivo Gliadin Stimulation of Intestinal Cells, Measuring intestinal permeability in celiac disease ex vivo using Ussing chambers, Intestinal permeability assessment using lactulose and mannitol, In vivo sensitization to gliadin by oral administration, Separation of epithelial and immune cells from intestinal biopsy samples, Isolation and study of dendritic cells and macrophages from human intestinal samples, Gnobiotic mouse models to study gluten sensitivity, and more.
Other sections in this new release include In vitro differentiation of macrophages from peripheral blood cells of celiac patients, Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a three-day gluten challenge, Isolation, cryopreservation and thawing of peripheral blood mononuclear cells for downstream applications, and more.
Table of Contents
1. Pepsin trypsin digested gliadin treatment of intestinal cells in vitroAne Olazagoitia-Garmendia2. Ex vivo Gliadin Stimulation of Intestinal CellsChuan He3. Measuring intestinal permeability in celiac disease ex vivo using Ussing chambersAmaia Jauregi-Miguel4. Intestinal permeability assessment using lactulose and mannitolSilvia Martinez Velasco5. In vivo sensitization to gliadin by oral administrationLaura Herrero and Ainara Castellanos-Rubio6. Separation of epithelial and immune cells from intestinal biopsy samplesIrati Romero Garmendia7. Isolation and study of dendritic cells and macrophages from human intestinal samples.David Bernardo8. Gnobiotic mouse models to study gluten sensitivityHeather Galipeau, McMaster University and Elena Verdu9. In vitro differentiation of macrophages from peripheral blood cells of celiac patientsGiovanna Del Pozzo and Alessio Fasano10. Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a three-day gluten challengeConchita Nunez and Mercedes Rubio11. Isolation, cryopreservation and thawing of peripheral blood mononuclear cells for downstream applications.Mercedes Rubio and Conchita Nunez12. Flow cytometric analysis of duodenal intraepithelial lymphocytes (lymphogram): a diagnostic test for celiac diseaseConchita Nunez13. The HLA-DQ8 transgenic mouse: a model to study the immune and cytotoxic responses to wheat gliadinMauro Rossi14. Human Intestinal organoid models for celiac disease researchKeijo Viiri15. Preparation of pepsin trypsin digested gliadin for stimulation experimentsAinara Castellanos-Rubio16. A fast, cheap, and easy protocol for celiac disease HLA haplotype typing using oral swabsAinara Castellanos-Rubio
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