Modifications and targeting of protein termini
Author(s)
Bibliographic Information
Modifications and targeting of protein termini
（Methods in enzymology / editors-in-chief, Anna Marie Pyle, David W. Christianson, v. 686）
Academic Press, an imprint of Elsevier, 2023
- pt. B
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Note
Includes bibliographical references
Description and Table of Contents
Description
Modifications and Targeting of Protein Termini, Part B, Volume 686 in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters on a variety of timely topics, including In vitro production of N-degron fused proteins and its application, Identification of N-degrons and N-recognins using peptide pull-downs combined with quantitative mass spectrometry-based proteomics, Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteins, Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathway, Characterization and chemical modulation of p62/SQSTM1/Sequestosome-1 as an autophagic N-recognin of the Arg/N-degron pathway.
Other chapters cover Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae, Building libraries to dissect terminal degrons with fluorescent timers, Affinity isolation and biochemical characterization of N-degron ligands using the N-recognin, ClpS, Probing the effects of N-terminal acetylation on a-synuclein structure, aggregation and toxicity, Increasing the coverage of the N-terminome with Lys-N Amino Terminal enrichment (LATE), and more.
Table of Contents
1. In vitro production of N-degron fused proteins and its applicationHyun Kyu Song, Bong Heon Kim, Leehyeon Kim, Do Hoon Kwon and Woo Seok Yang2. Identification of N-degrons and N-recognins using peptide pull-downs combined with quantitative mass spectrometry-based proteomicsFranziska Muller and Tanja Bange3. Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteinsThomas P. Keeley4. Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathwayYong Tae Kwon, Ah Jung Heo, Soon Chul Kwon and Jihoon Lee5. Characterization and chemical modulation of p62/SQSTM1/Sequestosome-1 as an autophagic N-recognin of the Arg/N-degron pathwaySu Jin Lee, Hye Yeon Kim, Min Ju Lee, Su Bin Kim, Yong Tae Kwon and Chang Hoon Ji6. Characterization of degradation signals at protein C-terminiHsueh-Chi S. Yen, Chi-Wei Yeh, Wei-Chieh Huang, Shu-Yu Hsu, Ching-Yu Chu and Lo-Tung Lee7. Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiaeJessica Boehm, Nikola Winter, Aida Kozlic, Theresia Telser, Lilian Nehlin and Andreas Bachmair8. Building libraries to dissect terminal degrons with fluorescent timersAnton Khmelinskii, Ka-Yiu Edwin Kong, Christian Reinbold and Michael Knop9. Affinity isolation and biochemical characterisation of N-degron ligands using the N-recognin, ClpSKaye N. Truscott and David A. Dougan10. Probing the effects of N-terminal acetylation on a-synuclein structure, aggregation and toxicityJanet R. Kumita, Rosie Bell and Michele Vendruscolo11. Increasing the coverage of the N-terminome with Lys-N Amino Terminal enrichment (LATE)Oded Kleifeld, Rawad Hanna, Andrey Rozenberg and Tali Lavy12. In vitro autoubiquitination activity of E3 ubiquitin ligases of the N-degron pathwayNico Dissmeyer13. Antibodies specific for N-terminal arginylationNico Dissmeyer14. TEV protease cleavage in generation of artificial substrate proteins bearing neo-N-terminiNico Dissmeyer and Lioba Inken Winckler15. Multiplexed protein stability (MPS) profiling of terminal degrons using fluorescent timer libraries in Saccharomyces cerevisiaeMichael Knop, Christian Reinbold, Ka-Yiu Edwin Kong, Ilia Kats and Anton Khmelinskii16. Using cell lysates to assess N-terminal acetyltransferase activity and impairmentThomas Arnesen, Nina McTiernan and Malin Lundekvam
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