Optical spectroscopic and microscopic techniques : analysis of biological molecules
著者
書誌事項
Optical spectroscopic and microscopic techniques : analysis of biological molecules
Springer, c2022
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注記
Includes bibliographical references
内容説明・目次
内容説明
This book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques.
目次
Chapter 1_Absorption Spectroscopy. -Chapter 2_Mapping DNA Conformations by Circular Dichroism Spectroscopy. -Chapter 3_Circular Dichorism Spectroscopy: Exploring the Protein secondary and tertiary structure. -Chapter 4_Protein/Ligand interaction using Steady-state fluorescence spectroscopy. -Chapter 5_Unraveling the biomolecular kinetics and dynamics using Fluorescence Lifetime. -Chapter 6_Fluorescence Anisotropy: Probing Rotational Dynamics of Biomolecules.- Chapter 7_From Ensemble FRET to Single molecule Imaging: Monitoring individual cellular machinery in action. -Chapter 8_Exploring supramolecular chemistry using fluorescence as a basic tool. -Chapter 9_Fluorescence Correlation Spectroscopy : A novel single molecule tool for Biomolecular applications. -Chapter 10_Fluorescence microscopy for Biomolecules: Principle and Applications.-Chapter 11_Biomolecular alteration in the disease associated protein repertoire revealed by FTIR spectroscopy. -Chapter 12_Advancement in Raman Spectroscopy towards Biomolecular structure and Dynamics.
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