Spectral and imaging cytometry : methods and protocols

著者

    • Barteneva, Natasha S.
    • Vorobjev, Ivan A.

書誌事項

Spectral and imaging cytometry : methods and protocols

edited by Natasha S. Barteneva, Ivan A. Vorobjev

（Methods in molecular biology / John M. Walker, series editor, 2635）

Humana Press, c2023

2nd ed.

大学図書館所蔵 件 / 全2件

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam - cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations. Cutting-edge and thorough, Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a valuable resources for beginners and professionals interested in learning more about this developing field. Chapters 1, 2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

目次

Dedication... Preface... Table of Contents... Contributing Authors... Part I Spectral Flow Cytometry 1. Development of Spectral Imaging Cytometry Ivan A. Vorobjev, Aigul Kussanova, and Natasha S. Barteneva 2. Using Virtual Filtering Approach to Discriminate Microalgae by Spectral Flow Cytometer Natasha S. Barteneva, Aigul Kussanova, Veronika Dashkova, Ayagoz Meirkhanova, and Ivan A. Vorobjev Part II Imaging Flow Cytometry - Imagestream 3. Imaging Flow Cytometric Analysis of Primary Bone Marrow Erythroblastic Islands Joshua Tay, Kavita Bisht, Ingrid G. Winkler, Jean-Pierre Levesque 4. Imaging Flow Cytometry of Legionella-Containing Vacuoles in Intact and Homogenized Wild-Type and Mutant Dictyostelium Amanda Welin, Dario Husler, and Hubert Hilbi 5. Imaging Flow Cytometry of Multi-Nuclearity Ivan A. Vorobjev, Sultan Bekbayev, Adil Temirgaliyev, Madina Tlegenova, and Natasha S. Barteneva 6. The Imaging Flow Cytometry-Based Cytokinesis-Block MicroNucleus (CBMN) Assay Ruth C. Wilkins, Matthew Rodrigues, and Lindsay A. Beaton-Green 7. High-Throughput -H2AX Assay Using Imaging Flow Cytometry Younghyun Lee, Qi Wang, Ki Moon Seong, and Helen C. Turner 8. Label Free Identification of Persistent Particles in Association with Primary Immune Cells by Imaging Flow Cytometry Bradley Vis, Jonathan J. Powell, and Rachel E. Hewitt 9. "Immuno-FlowFISH": Applications for Chronic Lymphocytic Leukaemia Henry Y.L. Hui, Wendy N. Erber, and Kathy A. Fuller 10. Quantifying Golgi Apparatus Fragmentation Using Imaging Flow Cytometry Inbal Wortzel and Ziv Porat 11. Flow Imaging of the Inflammasome: Evaluating ASC Speck Characteristics and Caspase-1 Activity Abhinit Nagar and Jonathan A. Harton 12. Quantitative Analysis of Latex Beads Phagocytosis by Human Macrophages Using Imaging Flow Cytometry with Extended Depth of Field (EDF) Ekaterina Pavlova, Daria Shaposhnikova, Svetlana Petrichuk, Tatiana Radygina, and Maria Erokhina Part III Imaging Flow Cytometry - FlowCam 13. FlowCam 8400 and FlowCam Cyano Phytoplankton Classification and Viability Staining by Imaging Flow Cytometry Kathryn H. Roache-Johnson and Nicole R. Stephens 14. Optimizing FlowCam Imaging Flow Cytometry Operation for Classification and Quantification of Microcystis Morphospecies Dmitry Malashenkov, Veronika Dashkova, Ivan A. Vorobjev, and Natasha S. Barteneva Subject Index List...

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