Viroids : methods and protocols

著者

• Rao, Ayala L. N.
• Lavagi-Craddock, Irene
• Vidalakis, Georgios

書誌事項

Viroids : methods and protocols

edited by Ayala L.N. Rao, Irene Lavagi-Craddock, and Georgios Vidalakis

（Methods in molecular biology / John M. Walker, series editor, 2316）（Springer protocols）

Humana Press, c2022

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume explores the latest methods used by researchers to study the detection, characterization, and various aspects of viroids. The chapters in this book are organized into seven parts and cover topics such as detection methods based on the biology of viroids; detection techniques based on electrophoresis and hybridization techniques; PCR-based techniques that provide high degrees of sensitivity; emerging area of nucleic acid sequence-based technology; and emerging techniques in viroid research such as RNA silencing, splicing, and viroid structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Viroids: Methods and Protocols is a valuable resource for researchers and graduate students who are working with viroid diseases.

目次

Preface... Table of Contents... Contributing Authors... Part I Biological Techniques 1. Study and Detection of Citrus Viroids in Woody Hosts Robert R. Krueger and Georgios Vidalakis 2. Detection and Characterization of Viroids via Biological Assays on Herbaceous Hosts Rosemarie W. Hammond 3. Analysis on RNA Motif-Based RNA Trafficking in Plants Heather N. Smith, Junfei Ma, and Ying Wang 4. Isolation and Transfection of Citrus Protoplasts with Citrus Exocortis Viroid Subhas Hajeri, James Ng, Jude Grosser, and Georgios Vidalakis Part II Electrophoresis and Hybridization Techniques 5. High-Throughput RNA Extraction from Citrus Tissues for the Detection of Viroids Tyler Dang, Fatima Osman, Jinbo Wang, Tavia Rucker, Sohrab Bodaghi, Shih-hua Tan, Deborah Pagliaccia, Irene Lavagi-Craddock, and Georgios Vidalakis 6. Extraction and Purification of Viroids from Herbaceous Hosts Rosemarie W. Hammond 7. Detection of Viroids by sPAGE Gel Electrophoresis Nuria Duran-Vila 8. Two-Dimensional Polyacrylamide-Gel Electrophoresis Analysis of Viroid RNAs Jose-Antonio Daros 9. A Simplified Dot-Blot Hybridization Protocol for Potato spindle tuber viroid Detection in Solanaceae Oxana Kekstidou, Christina Varveri, and Nikon Vassilakos 10. Gel Blot Hybridization for Viroids Pedro Serra 11. Tissue Print Hybridization for Detection and Characterization of Viroids Maria S. Kaponi, Teruo Sano, and Panayota E. Kyriakopoulou 12. Fluorescein-Based Electrophoretic Mobility Shift Assay Shachinthaka D. Dissanayaka Mudiyanselage and Ying Wang Part III PCR Techniques 13. Detection of Viroids by RT-PCR Nerida J. Donovan, Grant A. Chambers, and Mengji Cao 14. Detection of Viroids using RT-qPCR Subhas Hajeri, Georgios Vidalakis, and Raymond Yokomi 15. Multiplex RT-PCR Francesco Faggioli and Marta Luigi 16. Real-Time Detection of Viroids using Singleplex and Multiplex Quantitative Polymerase Chain Reaction Fatima Osman and Georgios Vidalakis 17. Reverse Transcription-Loop Mediated Isothermal AMPlification (RT-LAMP) at the Service of Viroid Detection Iraklis N. Boubourakas and Panayota E. Kyriakopoulou 18. SYBR (R) Green RT-qPCR for the Universal Detection of Citrus Viroids Georgios Vidalakis, Jinbo Wang, Tyler Dang, Tavia Rucker, Sohrab Bodaghi, Shi-hua Tan, Irene Lavagi-Craddock, Alexandra Syed, Gerardo Uribe, Yi Hsieh, and Joana Carbajal-Moreno 19. Detection of Avocado sunblotch and Other Viroids using RNA Filter Paper Capture and RT-PCR Deborah M. Mathews, Sohrab Bodaghi, James A. Heick, and J. Allan Dodds Part IV Cloning and Sequencing Techniques 20. Cloning and Sequencing of Viroids Rosemarie W. Hammond 21. QuantiGene Plex Assay: A Method for High-Throughput Multiplex Citrus Viroid Detection and Identification Tyler Dang, Jinbo Wang, Tavia Rucker, Sohrab Bodaghi, Irene Lavagi-Craddock, and Georgios Vidalakis 22. Culture-Independent Discovery of Viroids by Deep Sequencing and Computational Algorithms Ali Raza, Shou-Wei Ding, and Qingfa Wu 23. An In Silico Detection of a Citrus Viroid from Raw High Throughput Sequencing Data Tyler Dang, Andres Espindola, Georgios Vidalakis, and Kitty Cardwell Part V RNA Silencing Techniques 24. Detection of Viroid RNA and vd-siRNA in N. benthamiana Plants: Northern Blot Analyses for Viroid and vd-siRNAs Konstantina Katsarou, Nikoleta Kryovrysanaki, and Kriton Kalantidis Part VI Splicing Techniques 25. Production of Recombinant RNA in Escherichia coli Using Eggplant Latent Viroid as a Scaffold Beltran Ortola and Jose-Antonio Daros Part VII Structural Analysis 26. Predicting the Structure of a Viroid Gerhard Steger Subject Index List...

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詳細情報

• NII書誌ID(NCID): BD05797801
• ISBN:
  • 9781071614631
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York, N.Y.
• ページ数/冊数: xiii, 375 p.
• 大きさ: 27 cm
• 分類:
  • DC23 : 579.29
• 件名:
  • LCSH : Viroids
• 親書誌ID:
  • BA10794673
  • BA88873632