Induced pluripotent stem (iPS) cells : methods and protocols
著者
書誌事項
Induced pluripotent stem (iPS) cells : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2454）（Springer protocols）
Humana Press, c2022
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This extensive new edition presents protocols reflecting the great strides made in the study of induced pluripotent stem (iPS) cells. The collection explores new and improved methods for the generation, expansion, and maintenance of iPS cells from different tissue types, characterization of their differentiation pathways along different lineages, and their potential utility in tissue repair and regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols, Second Edition aims to arm stem cell biologists, both novice and expert, with invaluable protocols that are currently being used in various laboratories around the world.
目次
1. Human Induced Pluripotent Stem Cell (iPSC) Handling Protocols: Maintenance, Expansion, and Cryopreservation
Davide Marotta, Chandrika Rao, and Valentina Fossati
2. Generation of Induced Pluripotent Stem Cells from Human Bone Marrow-Derived Mesenchymal Stem Cells
Duygu Koyuncu Irmak and Erdal Karaoz
3. Analysis of Clonal Composition in Human iPSC and ESC and Derived 2D and 3D Differentiated Cultures
Bernat del Olmo, Daria Merkurjev, Likun Yao, Mel lina Pinsach-Abuin, Ivan Garcia-Bassets, and Angels Almenar-Queralt
4. Culturing Human Pluripotent Stem Cells on Micropatterned Silicon Surfaces
Varvara Chalmantzi, Chara Simitzi, Angelos Papadopoulos, Eleni Bagli, Carol Murphy, Emmanuel Stratakis, and Theodore Fotsis
5. Porcine iPSC Generation: Testing Different Protocols to a Successful Application
Oriol Iborra-Egea, Daina Martinez-Falguera, Santiago Roura, Antoni Bayes-Genis, Angel Raya, and Carolina Galvez-Monton
6. Efficient High-Density hiPSCs Expansion in Simple Dialysis Device
Fuad Gandhi Torizal, Hyunjin Choi, Marie Shinohara, and Yasuyuki Sakai
7. Expanding the Differentiation Potential of Already-Established Pluripotent Stem Cells
Jose Gonzalez-Martinez and Marcos Malumbres
8. Generation of Quiescent Cardiac Fibroblasts Derived from Human Induced Pluripotent Stem Cells
Hao Zhang, Mengcheng Shen, and Joseph C. Wu
9. Inductive Co-Culture Differentiation of Induced Pluripotent Stem Cells into Cardiomyocytes
Axel J. Chu and Chinten James Lim
10. 3D Microwell Platform for Cardiomyocyte Differentiation of Human Pluripotent Stem Cells
Mariana A. Branco, Tiago P. Dias, Joao P. Cotovio, Carlos A.V. Rodrigues, Tiago G. Fernandes, Joaquim M.S. Cabral, and Maria Margarida Diogo
11. Scalable Generation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Sarkawt Hamad, Daniel Derichsweiler, Jurgen Hescheler, and Kurt Pfannkuche
12. Application of Human Induced Pluripotent Stem Cell Technology for Cardiovascular Regenerative Pharmacology
Qasim A. Majid, Barbara Orsolits, Lotta Pohjolainen, Zsofia Kovacs, Gabor Foeldes, and Virpi Talman
13. Efficient and Safe Method of Generating Induced Pluripotent Stem Cells from Human Skin Fibroblasts and Subsequent Differentiation into Functional Cardiomyocytes
Sheeja Rajasingh, Vinoth Sigamani, Narasimman Gurusamy, and Johnson Rajasingh
14. An Optical-Flow-Based Method to Quantify Dynamic Behavior of Human Pluripotent Stem Cell-Derived Cardiomyocytes in Disease Modeling Platforms
Mohammad Izadifar, Tunde Berecz, Agota Apati, and Andras Nagy
15. Production of Innervated Skeletal Muscle Fibers Using Human Induced Pluripotent Stem Cells
Megane Delourme, Natacha Broucqsault, Kilian Mazaleyrat, and Frederique Magdinier
16. Generation of Human Neural Progenitors from Blood Samples by Interrupted Reprogramming
Eszter Szabo, Flora Juhasz, Laszlo Homolya, Zsuzsa Erdei, and Agota Apati
17. Robust and Highly Efficient Protocol for Differentiation of Human Pluripotent Stem Cells into Mesenchymal Stem Cells
Manale Karam and Essam M. Abdelalim
18. The Development of Tissue Engineering Scaffolds Using Matrix from iPS-Reprogrammed Fibroblasts
Francesco Santarella, Fergal J. O'Brien, Jonathan A. Garlick, and Cathal J. Kearney
19. 3D Organoid Culture Using Skin Keratinocytes Derived from Human Induced Pluripotent Stem Cells
Tomoko Miyake and Mikio Shimada
20. Pluripotent Stem Cell Differentiation towards Functional Basal Stratified Epithelial Cells
Eduardo Soares and Huiqing Zhou
21. Serum-Free Production of Three-Dimensional Hepatospheres from Pluripotent Stem Cells
Hassan Rashidi and David C. Hay
22. The Differentiation of Human Induced Pluripotent Stem Cells into Podocytes In Vitro
Andrea F. Wise, Sheetal Saini, and Sharon D. Ricardo
23. A Method for Encapsulation and Transplantation into Diabetic Mice of Human Induced Pluripotent Stem Cells (hiPSC)-Derived Pancreatic Progenitors
Luiza Ghila, Thomas Aga Legoy, and Simona Chera
24. Highly Efficient Differentiation of Human Pluripotent Stem Cells into Pancreatic Progenitors Co-Expressing PDX1 and NKX6.1
Bushra Memon and Essam M. Abdelalim
25. Derivation of Three Dimensional Human Induced Pluripotent Stem Cell Derived Vocal Fold Mucosa for Clinical and Pharmacological Applications
Lungova Vlasta and Susan L. Thibeault
26. Generation of Human Induced Pluripotent Stem Cells Using Endothelial Progenitor Cells Derived from Umbilical Cord Blood and Adult Peripheral Blood
Xiugong Gao, Jeffrey J. Yourick, and Robert L. Sprando
27. Development of a Blood Brain Barrier Permeability Assay Using Human Induced Pluripotent Stem Cell Derived Brain Endothelial Cells
Claudie Charlebois, Jez Huang, Caroline Sodja, Maria Ribecco-Lutkiewicz, Ewa Baumann, Danica B. Stanimirovic, and Anna Jezierski
28. Revised "hPSC-Sac Method" for Simple and Efficient Differentiation of Human Pluripotent Stem Cells to Hematopoietic Progenitor Cells
Akinori Yuzuriha and Koji Eto
29. Efficient Generation of iPSC-Derived Hematoendothelial Progenitors and Specification toward T-Cell Lineage
Siriwal Suwanpitak, Nutchanawan Promnakhon, Ratchapong Netsrithong, and Methichit Wattanapanitch
30. Derivation and Characterization of Mesenchymal Stem Cells from iPS Cells
Qingguo Zhao and Fei Liu
31. Human Pluripotent Stem Cell Differentiation to Microglia
Laraib Ijaz, Madhura Nijsure, and Valentina Fossati
32. Human Induced Pluripotent Stem Cell-Derived Microglia (hiPSC-Microglia)
Amanda McQuade and Mathew Blurton-Jones
33. Isolation and Culture of Human Induced Pluripotent Stem Cell-Derived Cerebral Organoid Cells
Yasheng Yan, Thiago Arzua, Sarah Logan, and Xiaowen Bai
34. A Simple Method for Generating, Clearing, and Imaging Pre-Vascularized 3D Adipospheres Derived from Human iPS Cells
Xi Yao and Christian Dani
35. A Method for In Vitro Fabrication of Hybrid Bone/Cartilage Tissue Using Mouse Induced Pluripotent Stem Cells
Phoonsuk Limraksasin and Hiroshi Egusa
36. Differentiation of Human Induced Pluripotent Stem Cells (hiPSC) into Endothelial- Type Cells and Establishment of an In Vitro Blood-Brain Barrier Model
Yuan Li, Georg C. Terstappen, and Wandong Zhang
37. CRISPR/Cas9-Mediated Introduction of Specific Heterozygous Mutations in Human Induced Pluripotent Stem Cells
Karina O. Brandao, Catarina Grandela, Loukia Yiangou, Christine L. Mummery, and Richard P. Davis
38. CRISPR/Cas9-Mediated Gene Knock-Out and Knock-In Human iPSCs
Paolo Petazzi, Pablo Menendez, and Ana Sevilla
39. Generation of Monoclonal iPSC Lines with Stable Cas9 Expression and High Cas9 Activity
JinQi Liao, Guangqian Zhou, and Yan Zhou
40. CRISPR/Cas9-Mediated Genome Editing to Generate Clonal iPSC Lines
Carla Sanjurjo-Soriano, Nejla Erkilic, Daria Mamaeva, and Vasiliki Kalatzis
41. CRISPR/Cas9 Ribonucleoprotein Complex-Mediated Efficient B2M Knockout in Human Induced Pluripotent Stem Cells (iPSCs)
Nontaphat Thongsin and Methichit Wattanapanitch
42. Embryonal Carcinoma and Glioblastoma Cell Lines Derived from Monkey Induced Pluripotent Stem Cells
Hirohito Ishigaki and Yasushi Itoh
43. Methods for Isolation and Reprogramming of Various Somatic Cell Sources into iPSCs
Shunit Neeman-Egozi, Polina Baskin, and Ofer Binah
44. The Characteristics of Human iPS Cells and siRNA Transfection under Hypoxia
Kouji Sugimoto and Yoshihiko Hayashi
45. mRNA-Based Reprogramming under Xeno-Free and Feeder-Free Conditions
Jakob Jeriha, Nikola Kolundzic, Preeti Khurana, Andrea Perez-Dominguez, and Dusko Ilic
46. Non-Modified RNA-Based Reprogramming of Human Dermal Fibroblasts into Induced Pluripotent Stem Cells
Immacolata Belviso, Franca Di Meglio, Veronica Romano, Stefania Montagnani, and Clotilde Castaldo
47. Efficient Induction of Primate iPS Cells Using a Combination of RNA Transfection and Chemical Compounds
Toshiaki Watanabe and Erika Sasaki
48. Generation and Cultivation of Transgene-Free Macaque and Baboon iPSCs under Chemically-Defined Conditions
Ignacio Rodriguez-Polo, Michael Stauske, and Rudiger Behr
49. Generation of Marmoset Monkey iPSCs with Self-Replicating VEE-mRNAs in Feeder-Free Conditions
Stoyan G. Petkov and Rudiger Behr
50. Differentiation of Human Induced Pluripotent Stem Cells into Definitive Endoderm Using Simple Dialysis Culture Device
Hyun Jin Choi, Fuad Gandhi Torizal, Marie Shinohara, and Yasuyuki Sakai
51. Detecting and Modulating ER Stress to Improve Generation of Induced Pluripotent Stem Cells
Alejandro Fuentes-Iglesias, Cristina Ameneiro, Diana Guallar, and Miguel Fidalgo
52. Gene Editing in Human Induced Pluripotent Stem Cells Using Doxycycline-Inducible CRISPR-Cas9 System
Vasanth Thamodaran, Sonam Rani, and Shaji R. Velayudhan
53. Derivation of Clinical-Grade Induced Pluripotent Stem Cell Lines from Erythroid Progenitor Cells in Xenofree Conditions
Gurbind Singh, Kannan V. Manian, Chitra Premkumar, Alok Srivastava, Dolly Daniel, and Shaji R. Velayudhan
54. Generation of Murine Induced Pluripotent Stem Cells through Transposon-Mediated Reprogramming
Thirumala R. Talluri, Dharmendra Kumar, and Wilfried A. Kues
55. Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules
Seungmi Ryu, Pei-Hsuan Chu, Claire Malley, John Braisted, Pinar Ormanoglu, Ruili Huang, Misha Itkin, Zina Itkin, Paul Shinn, Carleen Klumpp-Thomas, Sam Michael, Carlos A. Tristan, Anton Simeonov, and Ilyas Singec
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