Plant membrane proteomics : methods and protocols
Author(s)
Bibliographic Information
Plant membrane proteomics : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1696）（Springer protocols）
Humana Press, c2018
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Includes bibliographical references and index
Description and Table of Contents
Description
This volume provides comprehensive and detailed protocols that discuss proteomic techniques, plant endosomes, and isolation of organelles and subcellular fractions. The chapters in this book explore numerous plant species and cover topics, such as isolation and purity assessment of membranes from Norway spruce; proteomic analysis of nuclei; analyzing the vacuolar membrane (tonoplast) proteome; isoforms of a thylakoid-bound protein; assay of plasma membrane H+-ATPase in plant tissue under abiotic stresses; and identification and characterization of plant membrane proteins using ARAMEMNON. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and thorough, Plant Membrane Proteomics: Methods and Protocols is a valuable resource that promotes the use of plant membrane proteomics to develop the future of the field.
Table of Contents
Preface...Table of Contents...Contributing Authors...
1. Free Flow Zonal Electrophoresis for Fractionation of Plant Membrane Compartments prior to Proteomic AnalysisBronwyn J. Barkla
2. Isolation and Purity Assessment of Membranes from Norway SpruceEnni Vaisanen, Junko Takahashi, Luis A. Jimenez Barboza, Xianbao Deng, Teemu H. Teeri, Kurt V. Fagerstedt, Sabine Luthje, and Anna Karkoenen
3. Nuclear Proteome: Isolation of Intact Nuclei, Extraction of Nuclear Proteins and 2-DE AnalysisAarti Pandey, Subhra Chakraborty, and Niranjan Chakraborty
4. Identification of Plant Nuclear Proteins Based on a Combination of Flow Sorting, SDS-PAGE, and LC-MS/MS AnalysisIvo Chamrad, Jana Urinovska, Beata Petrovska, Hana Jerabkova, Rene Lenobel, Jan Vrana, Jaroslav Dolezel, and Marek Sebela
5. Isolation, Purity Assessment, and Proteomic Analysis of NucleiSetsuko Komatsu
6. Proteomic Analysis of Rice Golgi Membranes Isolated by Floating through Discontinuous Sucrose Density GradientKazusato Oikawa, Takuya Inomata, Yoshitoshi Hirao, Tadashi Yamamoto, Marouane Baslam, Kentaro Kaneko, and Toshiaki Mitsui
7. Analyzing the Vacuolar Membrane (Tonoplast) ProteomeMiwa Ohnishi, Katsuhisa Yoshida, and Tetsuro Mimura
8. Preparation of Membrane Fractions (Envelope, Thylakoids, Grana and Stroma Lamellae) from Arabidopsis Chloroplasts for Quantitative Proteomic Investigations and Other StudiesLucas Moyet, Daniel Salvi, Martino Tomizioli, Daphne Seigneurin-Berny, and Norbert Rolland
9. Isolation of Intact Thylakoid Membranes from Heterocytes of Filamentous, Nitrogen-Fixing CyanobacteriaAnn Magnuson and Tanai Cardona
10. Targeted Quantification of Isoforms of a Thylakoid-Bound Protein: MRM Method DevelopmentRoque Bru-Martinez, Ascensio n Martinez-Ma rquez, Jaime Morante-Carriel, Susana Selle s-Marchart, Maria Jose Martinez-Esteso, Jose Luis Pineda-Lucas, and Ignacio Luque
11. Sample Preparation for Analysis of the Plant Mitochondrial membrane ProteomeChristine Schikowsky, Beate Thal, Hans-Peter Braun, and Holger Eubel
12. Plasma Membrane Proteomics of Arabidopsis Suspension Cultured Cells Associated with Growth Phase using Nano-LC-MS/MSBin Li, Daisuke Takahashi, Yukio Kawamura, and Matsuo Uemura
13. Mini-Scale Isolation and Preparation of Plasma Membrane Proteins from Potato Roots for LC/MS AnalysisAnna M. Jozefowicz, Andrea Matros, Katja Witzel, and Hans-Peter Mock
14. Assay of Plasma Membrane H+-ATPase in Plant Tissues under Abiotic StressesMalgorzata Janicka, Anna Wdowikowska, and Grazyna Klobus
15. Absolute Quantification of In Vitro Expressed Plant Membrane Proteins by Targeted Proteomics (MRM) for the Determination of Kinetic ParametersCarsten Rautengarten, Berit Ebert and Joshua L. Heazlewood
16. MSE for Label-Free Absolute Protein Quantification in Complex ProteomesStefan Helm and Sacha Baginsky
17. Identification and Characterization of Plant Membrane Proteins using ARAMEMNONRainer Schwacke and Ulf-Ingo Flugge
18. VANTED: A Tool for Integrative Visualization and Analysis of -Omics DataAnja Hartmann and Anna Maria Jozefowicz
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