Mechanobiology : methods and protocols

著者

• Zaidel-Bar, Ronen

書誌事項

Mechanobiology : methods and protocols

edited by Ronen Zaidel-Bar

（Methods in molecular biology / John M. Walker, series editor, 2600）（Springer protocols）

Humana Press, c2023

• hbk.

内容説明・目次

内容説明

This detailed book collects methodologies exploring mechanobiology, the involvement of mechanical forces in cell fate specification and in controlling single and collective cell behaviors such as directed migration, morphogenesis, wound healing, and the immune response. The volume features methods to quantify the mechanical properties of cells and adhesion proteins, to expose cells to external mechanical forces, to quantitatively characterize mechano-responses at various scales, to measure forces applied by cells on the extracellular matrix, as well as chapters on force measurement inside cells, probing cell signaling and gene expression in response to force, and biophysical modeling of cell shape and protein dynamics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mechanobiology: Methods and Protocols aims to provide meaningful tools for cell and developmental biologists approaching the study of cell and tissue dynamics from a mechanobiological perspective, molecular biologists interested in the effects of force on proteins, as well as for cancer biologists and biophysicists.

目次

Part I: Quantifying the Mechanical Properties of Cells and Adhesion Proteins 1. Measuring Cell Mechanical Properties Using Microindentation Julien Husson 2. In Situ Measurements of Cell Mechanical Properties Using Force-Spectroscopy Meital Reches 3. Quantification of Apparent Membrane Tension and Membrane-to-Cortex Attachment in Animal Cells Using Atomic Force Microscopy-Based Force Spectroscopy Martin Bergert and Alba Diz-Munoz 4. Characterizing the Biophysical Properties of Adhesive Proteins in Live Cells Using Single Molecule Atomic Force Microscopy Andrew Vae Priest and Sanjeevi Sivasankar Part II: Exposing Cells to External Mechanical Forces 5. Application of Shear Stress to Endothelial Cells Using a Parallel Plate Flow Chamber Jason Sedlak and Alisa Clyne 6. Cell Stretcher Assay to Analyze Mechanoresponses to Cyclic Stretching Robin Pullen, Jens Konrad, Bernd Hoffmann, and Rudolf Merkel 7. Two-Point Optical Manipulation of Cell Junctions in the Early Epithelium of the Drosophila Embryo Kenji Nishizawa, Claire Chardes, Raphael Clement and Pierre-Francois Lenne Part III: Quantitative Characterization of Cell Mechanosensing 8. A Microfluidic-Like System (MLS) to Grow, Image, and Quantitatively Characterize Rigidity-Sensing by Plant's Roots and Root Hair Cells David Pereira, Thomas Alline, Gaurav Singh, Marie-Edith Chaboute, and Atef Asnacios 9. Photoresponsive Hydrogels for Studying Mechanotransduction of Cells Keun-Young Park, David J. Odde, and Mark D. Distefano 10. Analyzing Mechanically Evoked Currents at Cell-Substrate Junctions Surabhi Shrestha, Jessica Richardson, and Kate Poole 11. Quantifying Strain Sensing Protein Recruitment During Stress Fiber Repair Shailaja Seetharaman, Stefano Sala, Margaret L. Gardel, and Patrick W. Oakes Part IV: Measuring Forces Applied by Cells on the Extracellular Matrix 12. Quantification of Invadopodia Formation and Matrix Degradation Activity Jubina Balan Venghateri and Benjamin Geiger 13. Measuring Cellular Traction Forces with Micropillar Arrays Malak Amer and Haguy Wolfenson 14. Imaging Cell Adhesive Force at the Single Molecule Level Ying Tu and Xuefeng Wang Part V: Force Measurement Inside Cells 15. Multiplexed Molecular Tension Sensor Measurements Using PIE-FLIM Lukas Windgasse and Carsten Grashoff 16. Visualizing Neurons under Tension In Vivo with Optogenetic Molecular Force Sensors Neus Sanfeliu-Cerdan, Li-Chun Lin, Alexander R. Dunn, Miriam B. Goodman, and Michael Krieg 17. Single-Cell Quantification of the Mechanical Stability of Cell-Cell Adherens Junction Using Glass Micropipettes Wenmao Huang, Chaoyu Fu, and Jie Yan Part VI: Probing Cell Signaling and Gene Expression in Response to Force 18. Using Micropatterned Supported Lipid Bilayers to Probe the Mechanosensitivity of Signaling Receptors Zhongwen Chen 19. Monitoring Mechano-Regulation of Gene Expression by RNA Sequencing Yekaterina A. Miroshnikova 20. Testing the Role of Focal Adhesion Kinase (FAK) in Topography-Mediated Stem Cell Differentiation by Inhibiting FAK Phosphorylation Yuan Yao and Evelyn K.F. Yim Part VII: Biophysical Modeling of Cell Shape and Protein Dynamics 21. Long-Term Fluorescence Recovery After Photobleaching (FRAP) Takumi Saito, Daiki Matsunaga, and Shinji Deguchi 22. Simulating 3D Cell Shape with the Cellular Potts Model Rabea Link and Ulrich S. Schwarz

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詳細情報

• NII書誌ID(NCID): BD08136668
• ISBN:
  • 9781071628508
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xi, 343 p.
• 大きさ: 27 cm
• 分類:
  • DC23 : 612.76
• 件名:
  • LCSH : Biomechanics
• 親書誌ID:
  • BA10794673
  • BA88873632