CiNii Articles - Kanaoka Yuichi

1

Syntheses of Nitro-Substituted Aryl Diazirines. An Entry to Chromogenic Carbene Precursors for Photoaffinity Labeling

HATANAKA Y. , HASHIMOTO Makoto , NAKAYAMA Hitoshi , KANAOKA Yuichi

3-Phenyl-3-trifluoromethyl-diazirine derivatives with nitro and alkoxy substituents on their aromatic ring have been synthesized as carbene precursors for chromogenic detection of photolabeled product …

Chem. Phar. Bull. 42, 826-831, 1994

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2

Characterization of Saccharide Moiety in the Electroplax Sodium Channel

NAKAYAMA Hitoshi , YAMAMOTO Shigeki , HATANAKA Yasumaru , HACHISU Tetsuaki , TSUNASAWA Susumu , SAKIYAMA Fumio , KANAOKA Yuichi

Carbohydrate chains on the large peptide of the voltage-sensitive sodium channel from Electrophorus electricus electroplax have been partially characterized by the lectin-blotting technique combined w …

Chemical & pharmaceutical bulletin 40(12), 3287-3291, 1992-12-25
3

Photoaffinity Labeling of the Electroplax Sodium Channel with a Photoreactive μ-Conotoxin Carrying a Radioactive and Chromogenic Diazirine

HATANAKA Yasumaru , NAKAYAMA Hitoshi , KANAOKA Yuichi

Photoreactive derivatives of μ-conotoxin GIIIA carrying a radioactive and chromogenic diazirine have been prepared as a photoaffinity labeling reagent for muscle-type sodium channels. When the reagent …

Chemical & pharmaceutical bulletin 40(9), 2537-2539, 1992-09-25
4

1991年度ノ-ベル医学・生理学賞--イオンチャンネル研究の画期的手法を開発 [in Japanese]

中山仁 , 金岡祐一

Chemistry 47(2), p113-116, 1992-02
5

Comparison of Deacylation Rates of Chymotryptic Catalysis within an Enantiomeric Pair of p-Nitrophenyl Esters

TANIZAWA Kazutaka , YAMADA Hiroyuki , ITOH Kunihiko , KANAOKA Yuichi

p-Nitrophenyl esters carrying a chiral acyl group were synthesized. These compounds were shown to meet the requirements of chymotrypsin for the specific binding and the acylation. Therefore, the behav …

Chemical & pharmaceutical bulletin 39(10), 2748-2749, 1991-10-25
6

Bimane Fluorogenic Substrates for Microdetermination of Angiotensin Converting Enzyme Level in Serum

SATO Eisuke , HATTORI Hiromi , NISHIKAWA Satoko , KANAOKA Yuichi

The fluorescence of 9,10-dioxa-syn-3,4,6,7-tetramethylbimane (bimane) was found to be quenched in the presence of nitrated aromatic amino acid. Bimane peptides containing nitrated amino acid (1a, b) w …

Chemical & pharmaceutical bulletin 39(8), 2146-2148, 1991-08-25

Ichushi Web
7

Synthesis of Diazipine and [^3H]Diazipine : Novel dihydropyridines as Photoaffinity Probes of Calcium Channels

TAKI Motohiko , KUNIYASU Akihiko , NAKAYAMA Hitoshi , KANAOKA Yuichi

Diazipine and [^3H]diazipine were synthesized as new 1,4-dihydropyridine photoaffinity ligands containing a phenyldiazirine group. After simple high performance liquid chromatography separation, both …

Chemical & pharmaceutical bulletin 39(7), 1860-1862, 1991-07-25
8

Introduction of γ-Glutamyl Residue into Chymotrypsin and Agarose Gel : Application to Cross-Linking and Immobilization of Protein

TANIZAWA Kazutaka , MIYAURA Takashi , KANAOKA Yuichi

In our previous work a new method for the cross-linking of protein was proposed. The method is based on the spontaneous chelate formation process between salicylaldehyde and α-amino acid residues. Thu …

Chemical & pharmaceutical bulletin 38(10), 2868-2870, 1990-10-25
9

Dansylation of Streptomyces Subtilisin Inhibitor : Spectrometric Analysis of the Fluorescence-Labeled Inhibitor

TANIZAWA Kazutaka , MORIYA Katsuhiro , KANAOKA Yuichi

Specific labeling of tyrosine residues of Streptomyces subtilisin inhibitor (SSI) was carried out by dansyl chloride. Analysis revealed that two tyrosine residues out of three in SSI were modified. Th …

Chemical & pharmaceutical bulletin 38(9), 2606-2609, 1990-09-25
10

Novel Fluorogenic Substrates for Phosphodiesterase I

SATO Eisuke , YOSHIKAWA Michi , KANAOKA Yuichi

The fluorescence of 9,10-dioxa-syn-3,4,6,7-tetramethylbimane (bimane) was found to be quenched in the presence of guanosine 5'-monophosphate. By using this phenomenon, the bimane system was used for t …

Chemical & pharmaceutical bulletin 38(8), 2287-2289, 1990-08-25
11

New Fluorogenic Substrates for Microdetermination of Cathepsin C

SATO Eisuke , MATSUDA Kohki , KANAOKA Yuichi

As a sensitive fluorometric assay for the activity of cathepsin C, bimane-peptides containing tryptophan were synthesized and shown to be fluorogenic substrates for the microdetermination of cathepsin …

Chemical & pharmaceutical bulletin 38(7), 2043-2044, 1990-07-25

Ichushi Web
12

生体機能解明のための化学合成 [in Japanese]

金岡祐一 [編]

化学増刊 (118), p1-253, 1990-06
13

Photochemistry of Conjugated Nitrogen-Thiocarbonyl Systems. VII. : Photoaddition of Quinoline-, Isoquinoline- and Phthalazine-Thione Systems to Olefins

SATO Eisuke , IKEDA Yoshiya , KANAOKA Yuichi

Photolyses of quinoline-2-thiones (1A, B), isoquinoline-1-thione (14A), and phthalazine-1-thiones (14B-D) in the presence of olefins (2) afforded photoaddition products such as substituted quinolines, …

Chemical & pharmaceutical bulletin 38(5), 1205-1210, 1990-05-25
14

Fluorescence Labeled and Cross-Linked Subtilisin : Kinetic Characteristics and Binding to Streptomyces Subtilisin Inhibitor

TANIZAWA Kazutaka , MANO Tsutomu , TONOMURA Ben'ichiro , HIROMI Keitaro , KANAOKA Yuichi

In the preceding paper, the preparation of fluorescent cross-linked subtilisin was described. In this paper we present the catalytic and binding properties of the modified enzyme. Kinetic analysis sho …

Chemical & pharmaceutical bulletin 38(2), 467-469, 1990-02-25
15

Preparation of Fluorescence-Labeled and Cross-Linked Subtilisin

TANIZAWA Kazutaka , MANO Tsutomu , KANAOKA Yuichi

Streptomyces subtilisin inhibitor (SSI) is a protein characterized by both its potent inhibitory activity toward subtilisin and its structure, composed of two homologous subunits. It binds two molecul …

Chemical & pharmaceutical bulletin 38(2), 464-466, 1990-02-25
16

ペプチド性貝毒コノトキシン--イオンチャンネル研究への化学的プロ-プ (1990年の化学-2-) [in Japanese]

畑中保丸 , 小林淳一 , 金岡祐一

Chemistry 45(2), p136-137, 1990-02
17

Synthesis of μ-Conotoxin GIIIA : A Chemical Probe for Sodium Channels

HATANAKA Yasumaru , YOSHIDA Eiichi , NAKAYAMA Hitoshi , KANAOKA Yuichi

μ-Conotoxin GIIIA, a 22 amino acid peptide paralytic toxin which inhibits the muscle voltage-activated sodium channels, was synthesized by a solid phase method. No purification of intermediates was ne …

Chemical & pharmaceutical bulletin 38(1), 236-238, 1990-01-25
18

膜電位依存性イオンチャンネル [in Japanese]

NAKAYAMA HITOSHI , HATANAKA YASUMARU , KANAOKA YUICHI

Farumashia 26(8), 798-803, 1990

J-STAGE Ichushi Web
19

日本薬学会学術賞受賞米光宰氏の業績 [in Japanese]

KANAOKA YUICHI

Farumashia 26(4), 369, 1990

J-STAGE
20

Photoaffinity Labeling of the Electroplax Sodium Channel with Tetrodotoxin Derivatives. II. : Comparison of the Photoreactivity of Different Photoactivable Group in the Tetrodotoxin Binding Site

YOSHIDA E. , NAKAYAMA Hitoshi , HATANAKA Yasumaru , KANAOKA Yuichi

Four photoactivable tetrodotoxin (TTX) derivatives and the corresponding tritiated compounds were synthesized and porified. The photoactivable groups introduced were a nitro-azidophenyl (NAP) group an …

Chem. Pharm. Bull. 38, 982-987, 1990

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金岡祐一 Kanaoka Yuichi

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金岡 祐一 Kanaoka Yuichi

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金岡祐一 Kanaoka Yuichi

Articles:　 1-20 of 69