Nakashima Toshihiro NAKAISHI Tomoyuki

ID:9000004146432

Department of Biotechnology, Graduate School of Engineering, Osaka University:The Chemo-Sero-Therapeutic Research Institute (2007年 CiNii収録論文より)

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Articles:  1-8 of 8

  • Effective Selection System for Experimental Evolution of Random Polypeptides Towards DNA-Binding Protein

    NAKASHIMA Toshihiro , TOYOTA Hitoshi , URABE Itaru , YOMO Tetsuya

    An experimental evolution with selection based on binding affinity to DNA was carried out on a library of phage-displayed random polypeptides of about 140 amino acid residues. First, we constructed a …

    Journal of bioscience and bioengineering 103(2), 155-160, 2007-02-25

    References (20)

  • Functional Qβ Replicase Genetically Fusing Essential Subunits EF-Ts and EF-Tu with β-Subunit

    KITA Hiroshi , CHO Junghwa , MATSUURA Tomoaki , NAKAISHI Tomoyuki , TANIGUCHI Ichiro , ICHIKAWA Takehiko , SHIMA Yasuhumi , URABE Itaru , YOMO Tetsuya

    Qβ replicase, an RNA-dependent RNA polymerase of RNA coliphage Qβ, is a heterotetramer composed of a phage-encoded β-subunit and three host-encoded proteins: the ribosomal protein S1 (& …

    Journal of Bioscience and Bioengineering 101(5), 421-426, 2006-05-25

    J-STAGE  DOI  References (28)

  • Kinetic Properties of Qβ Replicase, an RNA Dependent RNA Polymerase

    NAKAISHI Tomoyuki , IIO Kentaro , YAMAMOTO Keizo , URABE Itaru , YOMO Tetsuya

    The kinetic properties of Qβ replicase, an RNA-dependent RNA polymerase, were investigated experimentally. The reaction at the A-incorporation site was inhibited by UTP and CTP with inhibition constan …

    Journal of Bioscience and Bioengineering 93(3), 322-327, 2002-03-25

    References (15) Cited by (1)

  • Synthesis of Functional Protein in Liposome

    WEIYU , SATO Kanetomo , WAKABAYASHI Maki , NAKAISHI Tomoyuki , KO-MITAMURA Elizabeth P. , SHIMA Yasufumi , URABE Itaru , YOMO Tetsuya

    The liposome consisting of eggPC, cholesterol, and DSPE-PEG5000 with a molar ratio of 1.5:1:0.08 was used to entrap cell-free protein synthesis reaction mixture. The synthesis of a mutant green fluore …

    Journal of Bioscience and Bioengineering 92(6), 590-593, 2001-12-25

    J-STAGE  DOI  References (22) Cited by (5)

  • Construction and Characterization of Phage Libraries Displaying Artificial Proteins with Random Sequences

    NAKASHIMA TOSHIHIRO , ISHIGURO NAOKI , YAMAGUCHI MUNEYOSHI , YAMAUCHI ASAO , SHIMA YASUFUMI , NOZAKI CHIKATERU , URABE ITARU , YOMO TETSUYA

    Three phage libraries, PL1, PL2, and PL3, displaying artificial proteins with random sequences were constructed. The artificial proteins, which are model of ancestral proteins, are derivatives of the …

    Journal of Bioscience and Bioengineering 90(3), 253-259, 2000-09-25

    References (24) Cited by (4)

  • Purification and Characterization of Lipase from Rhizopus chinensis Cells

    YASUDA Masahiro , OGINO Hiroyasu , KIGUCHI Taro , KOTANI Takuya , TAKAKURA Sinji , ISHIBASHI Takuya , NAKASHIMA Toshimitsu , FUKUDA Hideki , ISHIKAWA Haruo

    Lipase from Rhizopus chinensis cells was purified and characterized. The molecular mass of purified lipase was 28.4 kDa and the optimal temperature and pH for its activity were 37℃ and 5.5, respective …

    Journal of Bioscience and Bioengineering 88(5), 571-573, 1999-11-25

    DOI  References (10) Cited by (1)

  • Colorimetric measurement of total activity of leucine aminopeptidase and arylamidase in serum using laccase-catalyzed oxidative decarboxylation

    Agematu Hitosi , Nakashima Takashi , Shibamoto Norio , Yoshioka Takeo , Shin Takashi , Murao Sawao

    A simple colorimetric method for measuring the total activity of leucine aminopeptidase (EC 3.4.11.1) and arylamidase (EC 3.4.11.2) in serum was developed. The assay method is based on the following s …

    Journal of Fermentation and Bioengineering 77(5), 479-482, 1994-01-01

    DOI 

  • Continuous interesterification of oils and fats using dried fungus immobilized in biomass support particles

    KYOTANI S. , Nakashima Toshimitsu , Izumoto Eiji , Fukuda Hideki

    The industrial feasibility of an interesterification process using acetone-dried fungus(as a lipase catalyst)immobilized in biomass support particles(BSPs)was examined by continous interesterification …

    J. Ferment. Bioeng. 71, 286-288, 1991

    DOI  Cited by (5)

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