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  • モノメチンシアニン蛍光色素による生細胞核小体RNAイメージング

    西澤 精一, 芳野 幸奈, 何 夢夢, 樋口 啓, 富樫 奈央, 鈴木 理志, 五十嵐 友梧, 佐藤 雄介 分析化学 73 (3), 79-86, 2024-03-05

    ...<p>一般に既存の核酸結合低分子化合物はDNA選択性を示すため,RNAイメージングに適用しうる蛍光性小分子(色素)の開発は試行錯誤的な状況にある.実際,市販されている核小体RNA染色色素はわずか2種類であり(化学構造は非公開),いずれも生細胞イメージングに適用することができない.DNA(核)染色に汎用される蛍光色素DAPIやヘキスト33342のように,生体膜透過性をもち細胞培養液に加えるだけで生きた...

    DOI

  • NODeJ: an ImageJ plugin for 3D segmentation of nuclear objects

    Tristan Dubos, Axel Poulet, Geoffrey Thomson, Emilie Péry, Frédéric Chausse, Christophe Tatout, Sophie Desset, Josien C. van Wolfswinkel, Yannick Jacob BMC Bioinformatics 23 (1), 216-, 2022-06-06

    ...We reanalyzed public datasets and determined that NODeJ is able to accurately identify heterochromatin domains from a diverse set of <jats:italic>Arabidopsis thaliana</jats:italic> nuclei stained with DAPI...

    DOI PDF Web Site 被引用文献1件

  • 近年、北海道で見出された細菌性広葉樹病害(Ⅰ)

    石原 誠 日本森林学会大会発表データベース 133 (0), 654-, 2022-05-30

    ...患部を解剖してDAPIによる蛍光観察をすると、黄化葉の中助部と2年生枝の壊死部近傍の師部細胞内にDNAの蓄積による発光が観察されたことから、ファイトプラズマの鑑別性状と一致した。現在、病原の検出について検討を行っている。</p>...

    DOI

  • Arctigenin Triggers Apoptosis and Autophagy via PI3K/Akt/mTOR Inhibition in PC-3M Cells

    Sun Bai-ling, Cai En-bo, Zhao Yan, Wang Yu, Yang Li-min, Wang Jing-Yao CHEMICAL & PHARMACEUTICAL BULLETIN 69 (5), 472-480, 2021-05-01

    ...DAPI staining and flow cytometry showed that ARG induced PC-3M cell apoptosis and arrested cell in G0/G1 phase. Acridine orange staining showed that ARG induced autophagy in PC-3M cells....

    DOI Web Site Web Site ほか2件 被引用文献3件 参考文献49件

  • Fluorescent Cytochemical Detection of Polyphosphates Associated with Human Platelets

    Atsushi Sato, Hachidai Aizawa, Tetsuhiro Tsujino, Kazushige Isobe, Taisuke Watanabe, Yutaka Kitamura, Tomoyuki Kawase International Journal of Molecular Sciences 22 (3), 1040-, 2021-01-21

    ...Based on the results obtained by using activated platelets, treatment with alkaline phosphatases, and serotonin release, the DAPI+ targets were identified as polyP....

    DOI Web Site 被引用文献4件 参考文献23件

  • A twin-axial pseudorotaxane for phosphorescence cell imaging

    Xin-Kun Ma, Ying-Ming Zhang, Qilin Yu, Haoyang Zhang, Zhiyuan Zhang, Yu Liu Chemical Communications 57 (10), 1214-1217, 2021

    ...<p>A twin-axial pseudorotaxane is constructed using a phenylpyridine salt with diethanolamine (DA-PY) and cucurbit[8]uril (CB[8]), and it not only displays phosphorescence in aqueous solution but it can...

    DOI Web Site 被引用文献1件

  • Stretch-Induced Activation of Pannexin 1 Channels Can Be Prevented by PKA-Dependent Phosphorylation

    Ximena López, Rosalba Escamilla, Paola Fernández, Yorley Duarte, Fernando González-Nilo, Nicolás Palacios-Prado, Agustín D. Martinez, Juan C. Sáez International Journal of Molecular Sciences 21 (23), 9180-, 2020-12-02

    ...The mechanical stretch-induced activity—measured by changes in DAPI uptake—of Panx1 channels expressed in HeLa cell transfectants was inhibited by adenosine or cAMP analogs that permeate the cell membrane...

    DOI Web Site 被引用文献1件

  • Ionizing Irradiation Induces Vascular Damage in the Aorta of Wild-Type Mice

    Nobuyuki Hamada, Ki-ichiro Kawano, Farina Mohamad Yusoff, Kyoji Furukawa, Ayumu Nakashima, Makoto Maeda, Hiroshi Yasuda, Tatsuya Maruhashi, Yukihito Higashi Cancers 12 (10), 3030-, 2020-10-18

    ...Immunofluorescence staining showed that irradiation led to partial loss of the aortic endothelium (evidenced by a lack of adhesion molecule CD31 and 4′,6-diamidino-2-phenylindole (DAPI) signals), a decrease...

    DOI Web Site 被引用文献1件

  • Tumor-Cell–Macrophage Fusion Cells as Liquid Biomarkers and Tumor Enhancers in Cancer

    Yariswamy Manjunath, David Porciani, Jonathan B. Mitchem, Kanve N. Suvilesh, Diego M. Avella, Eric T. Kimchi, Kevin F. Staveley-O’Carroll, Donald H. Burke, Guangfu Li, Jussuf T. Kaifi International Journal of Molecular Sciences 21 (5), 1872-, 2020-03-09

    ...In addition to the traditional FDA-approved definition of a CTC (CD45-, EpCAM+, cytokeratins 8+, 18+ or 19+, with a DAPI+ nucleus), an additional circulating cell population has been identified as being...

    DOI Web Site 被引用文献1件

  • Tenocytes form a 3‐D network and are connected via nanotubes

    Monika Egerbacher, Simone Gabner, Sarah Battisti, Stephan Handschuh Journal of Anatomy 236 (1), 165-170, 2019-09-30

    ...Whole mounts of rat tail tendon fascicles (RTTfs) and sections of Achilles, flexor, extensor and patellar tendons were stained with Deep Red plasma membrane and DAPI nuclear stain and immunolabelled with...

    DOI Web Site Web Site ほか1件 被引用文献1件

  • Differential Chromosome Banding of Three <i>Rauvolfia</i> Species

    Md. Harun Ar Rashid, Chandan Kumar Dash, Sheikh Shamimul Alam, Syeda Sharmeen Sultana CYTOLOGIA 84 (2), 143-146, 2019-06-25

    ...No satellite was observed in the three species after orcein and DAPI-banding. However, a pair of satellites was found in <i>R. vomitoria</i> and <i>R. tetraphylla</i> after CMA-staining....

    DOI Web Site 参考文献11件

  • Comparative Chromosome Analysis of Three <i>Amaranthus</i> Species

    Fayizun Nahar Toma, Ishrat Jahan Bonna, Riyad Hossen, Sheikh Shamimul Alam, Syeda Sharmeen Sultana CYTOLOGIA 84 (2), 147-151, 2019-06-25

    ...In the three species, out of 22 CMA-banded and 27 DAPI-banded chromosomes, seven and eight were entirely fluoresced with CMA and DAPI, respectively....

    DOI Web Site 参考文献19件

  • Adverse Effects in Skeletal Muscle Following the Medicinal Use of Nicotiana glauca

    Musso Florencia, Lincor Darío, Vasconsuelo Andrea, Pronsato Lucía, Faraoni Belén, Milanesi Lorena Biological & Pharmaceutical Bulletin 42 (5), 671-679, 2019-05-01

    ...effects of extracts in C2C12 cells were analysed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL), Mitotracker and 4′,6-diamidino-2-phenylindole (DAPI...

    DOI Web Site Web Site ほか2件 参考文献31件

  • Karyotype Diversity in Three Medicinal <i>Piper</i> Species in Bangladesh

    Foysol Md. Arif, Bonna Ishrat Jahan, Alam Sheikh Shamimul, Sultana Syeda Sharmeen CYTOLOGIA 84 (1), 63-67, 2019-03-25

    ...The three species of <i>Piper</i> showed distinct chromomycin A<sub>3</sub> (CMA)- and 4′,6-diamidino-2-phenylindole (DAPI)-band patterns based on the number, location, and size of CMA- and DAPI-bands....

    DOI Web Site 参考文献25件

  • Vocabulary Learning Support System based on Automatic Image Captioning Technology

    緒方, 広明, Flanagan, Brendan, Akcapinar, Gokhan, Ogata, Hiroaki, Mouri, Kousuke, Uosaki, Noriko Distributed, Ambient and Pervasive Interactions (LNCS, volume 11587) 346-358, 2019

    Learning context has evident to be an essential part in vocabulary development, however describing learning context for each vocabulary is considered to be difficult. In the human brain, it is …

    HANDLE

  • Seamless Collaborative Learning Method to Learn Business Japanese with eBook and Chat System

    緒方, 広明, Mouri, Kousuke, Suzuki, Fumiya, Hasnine, Mohammad Nehal, Yonekawa, Takahiro, Yin, Chengjiu, Ogata, Hiroaki Distributed, Ambient and Pervasive Interactions (LNCS, volume 11587) 442-458, 2019

    The breakthrough of information technology has accelerated the evolutionary change in teaching and learning methodologies. In particular, pervasion of high-efficiency smartphones has raised the …

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  • DNA directed damage using a brominated DAPI derivative

    Elyse M. Digby, Rahul Rana, Mark Nitz, Andrew A. Beharry Chemical Communications 55 (67), 9971-9974, 2019

    ...<p>Conversion of a DNA-binding fluorophore (DAPI) to a photosensitizer <italic>via</italic> bromination retains high fluorescence and high affinity DNA binding but now produces light-induced reactive oxygen...

    DOI Web Site 被引用文献1件

  • Ratiometric DNA sensing with a host–guest FRET pair

    Shuai Zhang, Khaleel I. Assaf, Chusen Huang, Andreas Hennig, Werner M. Nau Chemical Communications 55 (5), 671-674, 2019

    ...<p>A host–guest FRET pair based on a carboxyfluorescein-labelled cucurbit[7]uril and DAPI was developed to sense DNA ratiometrically.</p>...

    DOI Web Site 被引用文献2件

  • マウス1細胞期胚のクロマチン構造へのリンカーヒストン変異体の関与

    船屋 智史, 青木 不学 日本繁殖生物学会 講演要旨集 112 (0), P-52-P-52, 2019

    ...またH1dを過剰発現した1細胞期胚において,DAPI染色を行った結果,一部で異常に凝集した前核が観察された。これらの結果より,1細胞期胚ではH1fooが高発現し,他の体細胞型リンカーヒストンが低発現していることにより,緩いクロマチン構造が形成されていることが示唆された。</p>...

    DOI

  • 異種間移植を想定したマウスPGCsの移動能の検討

    岩川 岳, 中谷 友紀, 中野 風子, 中村 啓哉, 谷藤 広志, 松原 和衛 日本繁殖生物学会 講演要旨集 112 (0), P-63-P-63, 2019

    ...その後,一次抗体に生殖細胞特異的な抗DDX4/MVH抗体(1,000倍希釈),二次抗体にAlexa594標識IgG(500倍希釈)とDAPI(1,000倍希釈)を用いて蛍光免疫染色を行い,マウスPGCsを観察した。また,孵卵6.5日目のニワトリ胚から採取したGRと12.5dpcのマウスGRを,トリプシンで細胞分散し,PGCsを含むGR由来細胞懸濁液をFCS添加培地で3から7日間共培養した。...

    DOI

  • ウシ超早期妊娠因子IgGのウシIVF胚に対する反応

    門岡 憲, 鈴木 幸太, 岩崎 節子, 片平 睦子, 中村 啓哉, 平田 統一, 松原 和衛 日本繁殖生物学会 講演要旨集 112 (0), P-76-P-76, 2019

    ...二次抗体は,Alexa594標識ロバ抗ウサギIgGを使用し,細胞核の標識にはDAPI を使用した。また,コントロールとして抗Oct3/4 IgGを用いた。さらに,各発生ステージの培養液を採取し,ウシ血清測定条件を基にしたSuper-EPF ELISAに適用した。...

    DOI

  • 4′,6-Diamidino-2-Phenylindole Distinctly Labels Tau Deposits

    Chengyu Li, Tetsuya Takahashi, Tejashwi Shrestha, Eiji Kinoshita, Tomoyasu Matsubara, Masayasu Matsumoto, Hirofumi Maruyama Journal of Histochemistry & Cytochemistry 66 (10), 737-751, 2018-08-14

    ...The peak emission wavelength of DAPI after binding to a tau deposit was similar to that after binding to a nucleus. This DAPI-positivity was conspicuous at the optimum concentration of 2 μg/ml....

    DOI Web Site Web Site 被引用文献1件

  • Improved Risk Stratification by Circulating Tumor Cell Counts in Pancreatic Cancer

    Katharina E. Effenberger, Cornelia Schroeder, Annkathrin Hanssen, Stefan Wolter, Christine Eulenburg, Michael Tachezy, Florian Gebauer, Jacob R. Izbicki, Klaus Pantel, Maximilian Bockhorn Clinical Cancer Research 24 (12), 2844-2850, 2018-06-14

    ...In peripheral blood, CTCs were identified by MACS enrichment (anti-cytokeratin/anti-EpCam) and subsequent automated analysis after combined anti-cytokeratin/anti-CD45/DAPI staining....

    DOI PDF 被引用文献2件

  • 11. 制限酵素阻害アッセイによるChromomycin A<sub>3</sub>の繰り返しDNAに対する同時結合性の評価(口頭発表の部)

    村瀬 裕貴, 野口 幹晴, 脇坂 元太郎, Wu Ting, 佐々木 茂貴 天然有機化合物討論会講演要旨集 60 (0), 61-66-, 2018

    ...しかしながら、DAPIなどの一般的なAT選択的マイナーグルーブ結合分子とは異なり、CMA3はGC選択性を有することから、現在もCMA3のDNA結合解析は精力的に研究されている。一方で、我々はDNA繰り返し配列に結合性を示す分子の開発を目指している。繰り返し配列は正常ゲノム中にも存在するが、この繰り返しの異常伸長により疾患を引き起こすことが知られている。...

    DOI

  • Image based Machine Learning for identification of macrophage subsets

    Hassan M. Rostam, Paul M. Reynolds, Morgan R. Alexander, Nikolaj Gadegaard, Amir M. Ghaemmaghami Scientific Reports 7 (1), 3521-, 2017-06-14

    ...Fluorescent microscopy was used to assess cell morphology of different cell types which were stained for nucleus and actin distribution using DAPI and phalloidin respectively....

    DOI PDF Web Site 被引用文献3件

  • Pathogenic Role of Associated Adherent‐Invasive <i>Escherichia coli</i> in Crohn's Disease

    Giuseppe Mazzarella, Angelica Perna, Angela Marano, Angela Lucariello, Vera Rotondi Aufiero, Alida Sorrentino, Raffaele Melina, Germano Guerra, Fabio Silvio Taccone, Gaetano Iaquinto, Antonio De Luca Journal of Cellular Physiology 232 (10), 2860-2868, 2017-05-15

    ...Moreover, on Caco2 cells, we analyzed the cell cycle, the expression of MGMT and DNMT1 genes, and DNA damage induced by LF82 and O83:H1, by FACS, RT‐PCR, and DAPI staining, respectively....

    DOI Web Site Web Site 被引用文献1件

  • A Novel Method of DAPI Staining for Differential Diagnosis of Renal Amyloidosis

    Matsuura Motokazu, Abe Hideharu, Tominaga Tatsuya, Sakurai Akiko, Murakami Taichi, Kishi Seiji, Bando Yoshimi, Minakuchi Jun, Nagai Kojiro, Doi Toshio The Journal of Medical Investigation 64 (3.4), 217-221, 2017

    ...Therefore, we propose that DAPI staining readily distinguishes AL renal amyloidosis from AA renal amyloidosis as a simple and reproducible histochemical method. J. Med....

    DOI Web Site PubMed ほか1件 被引用文献1件 参考文献16件

  • ラット末梢神経損傷モデルにおける経時的運動介入が神経再生に与える影響

    桑原 希望, 白勢 陽子, 中本 幸太, 木曽 波音, 国分 貴徳, 村田 健児, 金村 尚彦 理学療法学Supplement 2016 (0), 0629-, 2017

    ...一時抗体として抗Neurofilament抗体,二次抗体としてCy3,DAPIにより核染色で蛍光免疫組織化学染色を行った。その後,蛍光顕微鏡KEYENCEにて坐骨神経を撮影し,画像解析ソフトで単位面積当たりの平均輝度と単位面積当たりの個数を計測した。一元配置分散分析,多重比較検定Tukey法を行った。...

    DOI

  • 非荷重環境下でのレモンマートル摂取及び電気刺激が筋衛星細胞の活性化に与える効果

    松本 智博, 平林 卓己, 前田 拓寛, 中西 亮介, 本田 真一, 前重 伯壮, 藤野 英己 理学療法学Supplement 2016 (0), 0458-, 2017

    ...抗Pax7/抗Dystrophin/DAPIによる多重免疫染色で筋線維数当たりの筋核数及び筋衛星細胞数を算出した。筋衛星細胞の活性化の指標として抗MyoD/DAPIや筋衛星細胞の分化の指標として抗myogenin/DAPIの陽性核を測定した。統計処理には二元配置分散分析とTukeyの多重比較検定を使用し,有意水準は5%未満とした。...

    DOI

  • Karyotype Diversity in Three <i>Asparagus</i> L. Species

    Akter Suma, Begum Kazi Nahida, Sultana Syeda Sharmeen, Alam Sheikh Shamimul CYTOLOGIA 82 (5), 551-557, 2017

    ...Some unique CMA- and DAPI-banded chromosomes were found in three species which could be used as marker chromosomes for the respective species....

    DOI Web Site 被引用文献2件 参考文献20件

  • クロレラ藻類バイオ:リン,デンプン,オイル,オートファジー

    大田 修平, 河野 重行 PLANT MORPHOLOGY 29 (1), 57-61, 2017

    <p>バクテリアをはじめ,原生生物,藻類,動物の中には環境中の豊富なリンをポリリン酸の形で蓄積する生物が知られている.下水中の汚泥からリンを生物学的に除去するのを促進するために,ポリリン酸を蓄積するバクテリアが利用されている.クロレラを透過型電子顕微鏡で観察すると,電子密度が高い構造体が確認される.DAPIによる観察では,この電子密度の高い構造体は,形状や細胞内分布がポリリン酸を含む小胞と類似し…

    DOI Web Site Web Site 参考文献11件

  • Development of a Novel c-MET–Based CTC Detection Platform

    Tian Zhang, Rengasamy Boominathan, Brad Foulk, Chandra Rao, Gabor Kemeny, John H. Strickler, James L. Abbruzzese, Michael R. Harrison, David S. Hsu, Patrick Healy, Jing Li, Cinthia Pi, Katherine M. Prendergast, Carey Hobbs, Sarah Gemberling, Daniel J. George, Herbert I. Hurwitz, Mark Connelly, Mariano A. Garcia-Blanco, Andrew J. Armstrong Molecular Cancer Research 14 (6), 539-547, 2016-06-01

    ...Immunofluorescence was used to characterize cells for c-MET, DAPI, and pan-CK, excluding CD45+ leukocytes....

    DOI PDF 被引用文献1件

  • Pneumolysin activates neutrophil extracellular trap formation

    J G Nel, A J Theron, C Durandt, G R Tintinger, R Pool, T J Mitchell, C Feldman, R Anderson Clinical and Experimental Immunology 184 (3), 358-367, 2016-03-02

    ...These procedures were complemented by fluorescence microscopy using 4′, 6-diamino-2-phenylindole (DAPI) (nuclear stain) in combination with anti-citrullinated histone monoclonal antibodies to visualize...

    DOI PDF Web Site ほか2件 被引用文献2件

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