検索結果 267 件

齊藤, 峻介, 森, 和俊 BIO-PROTOCOL 13 (16), 2023-08-20

...Genetically engineered fluorescent reporter proteins, such as G-CEPIA1er and GCaMP6f, localize to intracellular regions of interest (i.e., ER and cytoplasm) and emit green fluorescence upon binding to...

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Yan Zhang, Márton Rózsa, Yajie Liang, Daniel Bushey, Ziqiang Wei, Jihong Zheng, Daniel Reep, Gerard Joey Broussard, Arthur Tsang, Getahun Tsegaye, Sujatha Narayan, Christopher J. Obara, Jing-Xuan Lim, Ronak Patel, Rongwei Zhang, Misha B. Ahrens, Glenn C. Turner, Samuel S.-H. Wang, Wyatt L. Korff, Eric R. Schreiter, Karel Svoboda, Jeremy P. Hasseman, Ilya Kolb, Loren L. Looger Nature 615 (7954), 884-891, 2023-03-15

...Here we used large-scale screening and structure-guided mutagenesis to develop and optimize several fast and sensitive GCaMP-type indicators<jats:sup>3–8</jats:sup>....

DOI PDF Web Site 被引用文献2件

Shiori Koyama, Kiyomi Nakayama, Ayako Mochizuki, Masanori Dantsuji, Shiro Nakamura, Koutaro Maki, Tomio Inoue Journal of Oral Biosciences 65 (1), 126-131, 2023-03

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Sae Tanaka, Kazuhiro Aoki, Kazuharu Arakawa Proceedings of the National Academy of Sciences 120 (5), 2023-01-24

...It enables the introduction of GFP-fused proteins and genetically encoded indicators such as the Ca <jats:sup>2+</jats:sup> indicator GCaMP into tardigrade cells; consequently,...

DOI Web Site 参考文献56件

水田 恒太郎 日本薬理学雑誌 158 (2), 144-149, 2023

<p>アルツハイマー病（AD）患者の初期症状では，外出して帰り道がわからなくなるなど時空間の認知障害が挙げられる．空間認知に関わる脳部位として海馬が知られている．海馬には場所を認識して活動する場所細胞が存在する．この細胞の活動により個体は，空間を認知し目的地まで誘導すると考えられる．しかしながら，ADにおける空間認知障害と場所細胞を含む海馬神経回路の破綻との関係や，神経回路レベルの病態の進行につ…

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益田 緋里, 板井 駿, 倉科 佑太, 遠山 周吾, 尾上 弘晃 生体医工学 Annual61 (Abstract), 247_1-247_1, 2023

<p>心疾患は世界の死因の大部分を占め健康寿命の短縮にも大きく寄与している．医学の進歩により一定の治療は達成されているものの未だ根治は難しく，新たな治療法の確立が必須である．そこで近年，ヒト多能性幹細胞（iPS細胞）を用いた心臓モデルが，動物実験よりも有用な薬剤評価デバイスとして期待されている．このiPS細胞由来組織の実用化にあたり不可欠な要素が，心筋組織の成熟化及び成熟度の評価である．心臓の電…

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河合 洋幸, 近藤 誠 日本薬理学会年会要旨集 97 (0), 2-B-S27-1-, 2023

...The GCaMP fluorescence from MRN serotonergic neurons decreased in response to rewarding stimuli and increased after aversive stimuli....

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H. James McQuillan, Jenny Clarkson, Alexia Kauff, Su Young Han, Siew Hoong Yip, Isaiah Cheong, Robert Porteous, Alison K. Heather, Allan E. Herbison Nature Communications 13 (1), 7433-, 2022-12-02

...Direct measurement of gonadotropin-releasing hormone (GnRH) pulse generator activity in freely behaving mice with GCaMP photometry demonstrated striking estradiol-dependent plasticity in the frequency,...

DOI PDF Web Site 被引用文献2件

Shutaro Kobayashi, Kazunori O’Hashi, Masayuki Kobayashi Scientific Reports 12 (1), 2022-09-07

...investigated spontaneous cortical activities in sessions before and after electrical stimulation of the periodontal ligament (PDL) by applying wide-field and two-photon calcium imaging to anesthetized GCaMP6s...

DOI PDF Web Site 参考文献55件

坂本, 雅行, Ota, Keisuke, Kondo, Yayoi, Okamura, Michiko, Fujii, Hajime, Bito, Haruhiko STAR Protocols 3 (2), 101421-, 2022-06-17

...This protocol uses G-CaMP9a transgenic mice, which express a genetically encoded calcium indicator with high signal-to-noise ratio....

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Hannah S. Wirtshafter, John F. Disterhoft The Journal of Neuroscience 42 (22), 4538-4554, 2022-05-02

...<jats:p>Calcium imaging using GCaMP indicators and miniature microscopes has been used to image cellular populations during long timescales and in different task phases, as well as to determine neuronal...

DOI Web Site 被引用文献1件

Kevin, Dorgans, Da, Guo, Kiyoto, Kurima, Jeff, Wickens, Marylka Yoe, Uusisaari Frontiers in Cellular Neuroscience 16 825056-, 2022-04-27

...The potential of these tools is demonstrated by expressing the calcium sensor GCaMP6s in adult mouse IO neurons....

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Franziska Bierbuesse, Anaïs C. Bourges, Vincent Gielen, Viola Mönkemöller, Wim Vandenberg, Yi Shen, Johan Hofkens, Pieter Vanden Berghe, Robert E. Campbell, Benjamien Moeyaert, Peter Dedecker Nature Communications 13 (1), 2022-04-06

...We develop proof-of-concept photochromic variants of the popular GCaMP family of Ca<jats:sup>2+</jats:sup> biosensors, and show that these can be used to resolve dynamic changes in the absolute Ca<jats...

DOI PDF Web Site 参考文献76件

Shi Zhou, Akira Yamashita, Jingyang Su, Yang Zhang, Wuyang Wang, Liying Hao, Akihiro Yamanaka, Tomoyuki Kuwaki Molecular Brain 15 (1), 21-, 2022-03-04

...TetO-GCaMP6 was then introduced into mice via an adeno-associated virus injection or natural crossing....

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Satoru Kondo, Yuko Kiyohara, Kenichi Ohki Frontiers in Neural Circuits 16 2022-02-28

...For this purpose, the genetically encoded calcium indicator, GCaMP6s, was locally expressed in the LPN using the adeno-associated virus (AAV) infection method....

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Fabio Miazzi, Kalpana Jain, Sabine Kaltofen, Jan E. Bello, Bill S. Hansson, Dieter Wicher Frontiers in Cellular Neuroscience 16 2022-02-24

...First, we established an <jats:italic>in vivo</jats:italic> preparation to image specific OSN population expressing the fluorescent Ca<jats:sup>2+</jats:sup> reporter GCaMP3 during OR activation with airborne...

DOI Web Site 被引用文献1件

坂本, 雅行, 井上, 昌俊, 竹内, 敦也, 小針, 成敬, 横山, 達士, 堀金, 慎一郎, 竹本(木村), さやか, 阿部, 学, 崎村, 建司, 狩野, 方伸, 喜多村, 和郎, 藤井, 哉, 尾藤, 晴彦 Cell Reports Methods 2 (2), 2022-02

...Combining this reporter mouse with viral or mouse genetic Flp delivery methods produces a robust and stable G-CaMP9a expression in defined neuronal populations without detectable detrimental effects....

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Masayuki Sakamoto, Masatoshi Inoue, Atsuya Takeuchi, Shigetaka Kobari, Tatsushi Yokoyama, Shin-ichiro Horigane, Sayaka Takemoto-Kimura, Manabu Abe, Kenji Sakimura, Masanobu Kano, Kazuo Kitamura, Hajime Fujii, Haruhiko Bito Cell Reports Methods 2 (2), 100168-, 2022-02

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水田 恒太郎 日本薬理学会年会要旨集 95 (0), 2-S16-2-, 2022

...Our chronic imaging using G-CaMP7-expressed AD mice could provide a novel view to evaluate the effects of a drug for AD progression.</p>...

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山本 亮, 古山 貴文, 趙 駸, 益岡 尚由, 堀 佳江, 伊藤 哲史, 小野 宗範, 加藤 伸郎 日本薬理学会年会要旨集 95 (0), 2-S20-3-, 2022

...Finally, we measured the activity of DAT^DR-PAG and VIP^DR-PAG neurons during the head-fixed classical fear conditioning by using the GCaMP photometry technique....

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董 彧弢, 溝口 博之, 山中 章弘, 山田 清文 日本薬理学会年会要旨集 95 (0), 1-SS-08-, 2022

...To measure the activities of orexin neurons, AAV-FLEX-GCaMP7s was injected into the hypothalamus of Orexin-Cre rats....

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齋藤 光象, 繁冨 英治, 小泉 修一 日本薬理学会年会要旨集 95 (0), 1-P-021-, 2022

...Using Iba1-GCaMP6f-hGFAP mice, an AxD model mice for microglial Ca²⁺ imaging, we studied microglial Ca²⁺ signals with 2 photon microscopy....

DOI Web Site

田中 里奈子, 羽田 和弘, 溝口 博之, 澤幡 雅仁, 永井 拓, 森 大輔, 鍋島 俊隆, 貝淵 弘三, 尾崎 紀夫, 山田 清文 日本薬理学会年会要旨集 95 (0), 2-P-168-, 2022

...We generated mice expressing selectivity GCaMP6 in dopamine D1 receptor-expressing MSNs (D1-MSNs) of the striatum by Cre-loxP system....

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齋藤 光象, 繁冨 英治, 篠崎 陽一, パラジュリ ビジェイ, 小泉 修一 日本薬理学会年会要旨集 96 (0), YIA08-5-, 2022

...In addition, using 2 photon imaging and Iba1-GCaMP6-60TM, an AxD model for microglial Ca²⁺ imaging, we recently found that microglial Ca²⁺ signals were also dramatically enhanced...

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髙村 侑希, 西村 京華, 人羅（今村） 菜津子, 南 雅文, 野村 洋 日本薬理学会年会要旨集 96 (0), 2-B-P-154-, 2022

...We introduced GCaMP6s into histamine neurons of HDC-Cre mice and acquired the fluorescence via optic fibers. The mice were subjected to auditory-reward conditioning....

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長沼 史登, 中村 正帆, ベトリベラン ラマリンガム, 吉川 雄朗, 岡村 信行 日本薬理学会年会要旨集 96 (0), 3-B-P-185-, 2022

...Next, we measured the neuronal activities of Nts-LH during sleep and wakefulness by using fiber photometory with a Ca²⁺ sensor, GCaMP7f....

DOI Web Site

Kobayashi Shutaro, O’Hashi Kazunori, Kaneko Keisuke, Kobayashi Satomi, Ogisawa Shouhei, Tonogi Morio, Fujita Satoshi, Kobayashi Masayuki Ｊｏｕｒｎａｌ　ｏｆ　Ｏｒａｌ　Ｓｃｉｅｎｃｅ 64 (2), 156-160, 2022

...Cortical imaging confirmed that type 1 expressed the cortical GCaMP6s, while type 2 did not....

DOI Web Site PubMed ほか1件 参考文献28件

田沼 将人, 宮司 渓汰, 大久保 仁, 野村 洋, 南 雅文, 橋本 均, 笠井 淳司 日本薬理学会年会要旨集 96 (0), YIA09-2-, 2022

...To address this question, here we performed calcium imaging of GCaMP6f-expressing claustral neurons in freely moving mice during three behavioral tests; the elevated plus maze, the open field test, and...

DOI Web Site

Kanuj Mishra, Juan Pablo Fuenzalida-Werner, Francesca Pennacchietti, Robert Janowski, Andriy Chmyrov, Yuanhui Huang, Christian Zakian, Uwe Klemm, Ilaria Testa, Dierk Niessing, Vasilis Ntziachristos, Andre C. Stiel Nature Biotechnology 40 (4), 598-605, 2021-11-29

...Here we constructed the prototype of a photo-switchable Ca<jats:sup>2+</jats:sup> sensor based on GCaMP5G that can be switched with 405/488-nm light and describe its molecular mechanisms at the structural...

DOI PDF Web Site 被引用文献1件

Claudia, Cecchetto, Stefano, Vassanelli, Bernd, Kuhn Frontiers in Neuroscience 15 741279-, 2021-11-11

...A chronic cranial window with access port allows injecting a viral vector expressing GCaMP6f or the voltage-sensitive dye (VSD) ANNINE-6plus, as well as entering the brain with a multi-channel neural probe...

DOI 機関リポジトリ Web Site 被引用文献1件

Yan Zhang, Márton Rózsa, Yajie Liang, Daniel Bushey, Ziqiang Wei, Jihong Zheng, Daniel Reep, Gerard Joey Broussard, Arthur Tsang, Getahun Tsegaye, Sujatha Narayan, Christopher J. Obara, Jing-Xuan Lim, Ronak Patel, Rongwei Zhang, Misha B. Ahrens, Glenn C. Turner, Samuel S.-H. Wang, Wyatt L. Korff, Eric R. Schreiter, Karel Svoboda, Jeremy P. Hasseman, Ilya Kolb, Loren L. Looger Neurophotonics 1 025008-, 2021-11-08

...The popular GCaMP indicators are based on the calcium-binding protein calmodulin and the RS20 peptide....

DOI Web Site 被引用文献1件

Frank K Lee, Jane C Lee, Bo Shui, Shaun Reining, Megan Jibilian, David M Small, Jason S Jones, Nathaniel H Allan-Rahill, Michael RE Lamont, Megan A Rizzo, Sendoa Tajada, Manuel F Navedo, Luis Fernando Santana, Nozomi Nishimura, Michael I Kotlikoff eLife 10 e67858-, 2021-10-29

...mouse crosses: optical cardiac pacing and simultaneous cardiomyocyte Ca<jats:sup>2+</jats:sup> imaging in <jats:italic>Hcn4</jats:italic><jats:sup>BAC</jats:sup>-CatCh2/<jats:italic>Myh6</jats:italic>-GCaMP8...

DOI PDF Web Site ほか1件 被引用文献1件

Yugo Miyata, Hiroto Fuse, Shoko Tokumoto, Yusuke Hiki, Ruslan Deviatiiarov, Yuki Yoshida, Takahiro G. Yamada, Richard Cornette, Oleg Gusev, Elena Shagimardanova, Akira Funahashi, Takahiro Kikawada Scientific Reports 11 (1), 19698-, 2021-10-05

...Using the Cas9-mediated gene knock-in system, we established Pv11 cells that stably expressed GCaMP3 to monitor intracellular Ca<jats:sup>2+</jats:sup> mobilization....

DOI PDF Web Site 被引用文献1件

Kei Kimura, Yuji Nagai, Gaku Hatanaka, Yang Fang, Andi Zheng, Maki Fujiwara, Mayuko Nakano, Yukiko Hori, Ryosuke Takeuchi, Mikio Inagaki, Takafumi Minamimoto, Ichiro Fujita, Ken-ichi Inoue, Masahiko Takada 2021-07-19

...To explore its applicability to chemogenetic manipulation and in vivo calcium imaging, the AAV2.1 vector expressing excitatory DREADDs or GCaMP was injected into the striatum or the visual cortex of macaque...

DOI Web Site 被引用文献4件

Ravi L. Rungta, Marc Zuend, Ali-Kemal Aydin, Éric Martineau, Davide Boido, Bruno Weber, Serge Charpak Communications Biology 4 (1), 855-, 2021-07-09

...we utilized a single whisker stimulation model to map out functional hyperemia along vascular arbours from layer II/III to the surface of primary somatosensory cortex, in anesthetized and awake Thy1-GCaMP6...

DOI PDF Web Site 被引用文献1件

Da, Guo, Ayşen, Gürkan Özer, Marylka Yoe, Uusisaari Journal of Visualized Experiments 172 e62222-, 2021-06-10

...location of the IO and involves a surgical procedure for inserting a gradient refractive index (GRIN) lens through the neck viscera to come into contact with theventral surface of the calcium sensor GCaMP6s-expressing...

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Alberto Muñoz, Margherita Bertuzzi, Constanze Seidel, Darren Thomson, Elaine M. Bignell, Nick D. Read Fungal Genetics and Biology 151 103470-, 2021-06

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Francesca Resentini, Matteo Grenzi, Daniele Ancora, Mara Cademartori, Laura Luoni, Marianna Franco, Andrea Bassi, Maria Cristina Bonza, Alex Costa Plant Physiology 187 (2), 603-617, 2021-05-29

...By exploiting state-of-the-art genetically encoded Ca2+ indicators, specifically the ER-GCaMP6-210 and R-GECO1, we report the generation and characterization of an Arabidopsis (Arabidopsis thaliana) line...

DOI PDF PDF 被引用文献1件

Romeo Rebusi, Joshua Philippe Olorocisimo, Jeric Briones, Yasumi Ohta, Makito Haruta, Hironari Takehara, Hiroyuki Tashiro, Kiyotaka Sasagawa, Jun Ohta Frontiers in Neuroscience 15 667708-, 2021-05-21

...We demonstrated the utility of the devices by using them on GCaMP6 mice in a formalin test experiment....

DOI Web Site 被引用文献4件 参考文献64件

Attila Kaszas, Gergely Szalay, Andrea Slézia, Alexandra Bojdán, Ivo Vanzetta, Balázs Hangya, Balázs Rózsa, Rodney O’Connor, David Moreau Scientific Reports 11 (1), 9775-, 2021-05-07

...Using intravital calcium imaging with the genetically encoded calcium indicator GCaMP6f, here we show that the application of infrared neural stimulation induces intracellular calcium signals in Layer...

DOI PDF Web Site 被引用文献1件

Hiromu Monai, Shinnosuke Koketsu, Yoshiaki Shinohara, Takatoshi Ueki, Peter Kusk, Natalie L. Hauglund, Andrew J. Samson, Maiken Nedergaard, Hajime Hirase Scientific Reports 11 (1), 2021-04-14

...Spontaneous neural activity and KCl-induced CSD were visualized by cortex-wide transcranial Ca<jats:sup>2+</jats:sup> imaging in G-CaMP7 transgenic mice....

DOI PDF Web Site 参考文献60件

Lawrence Huang, Peter Ledochowitsch, Ulf Knoblich, Jérôme Lecoq, Gabe J Murphy, R Clay Reid, Saskia EJ de Vries, Christof Koch, Hongkui Zeng, Michael A Buice, Jack Waters, Lu Li eLife 10 e51675-, 2021-03-08

...or GCaMP6f....

DOI PDF Web Site ほか1件 被引用文献2件

Chiaki Hoshino, Ayumu Konno, Nobutake Hosoi, Ryosuke Kaneko, Ryo Mukai, Junichi Nakai, Hirokazu Hirai Molecular Brain 14 (1), 33-, 2021-02-15

...To test the ability of the mGAD65 promoter to express a functional molecule, we virally expressed G-CaMP, a fluorescent Ca<jats:sup>2+</jats:sup> indicator, in the motor cortex, and this enabled us to...

DOI PDF Web Site 被引用文献2件 参考文献40件

Hidekazu Tanaka, Retsu Mitsui, Mitsuhiro Oishi, Stefan Passlick, Ronald Jabs, Christian Steinhäuser, Kenji F. Tanaka, Hikaru Hashitani British Journal of Pharmacology 178 (5), 1073-1094, 2021-02-02

...Intercellular Ca<jats:sup>2+</jats:sup> dynamics in pericytes or smooth muscle cells (SMCs) of suburothelial arterioles were visualised using transgenic mice expressing GCaMP6 under control of the NG2‐...

DOI Web Site Web Site ほか1件 被引用文献3件 参考文献54件

人羅（今村） 菜津子 日本薬理学会年会要旨集 94 (0), 2-S20-2-, 2021

...Fiber photometry using Ca²⁺-sensitive fluorescent protein GCaMP6 showed that neuronal pathway from the medial prefrontal cortex (mPFC) to the periaqueductal gray (PAG) was activated when mice...

DOI Web Site

西村 京華, 久保 絢女, 清水目 倫太郎, 人羅（今村） 菜津子, 南 雅文, 野村 洋 日本薬理学会年会要旨集 94 (0), 2-P1-09-, 2021

...We virally targeted GCaMP6, a genetically-encoded calcium indicator, was Cre-dependently expressed  into histamine neurons in the TMN of HDC-Cre mice, and implanted an optic fiber was implanted in the...

DOI Web Site

山下 哲 自律神経 58 (1), 116-120, 2021

<p>一般に，動物が急激なストレスに晒されると，心拍・呼吸・血圧の上昇，鎮痛作用などが引き起こされるが，これはストレス防衛反応と呼ばれている．交感神経系の賦活化により引き起こされるこれらの反応は，例えば天敵に遭遇した場合などに動物個体の生存確率を上げるために必要不可欠な反応である．以前の研究で，オレキシン欠損マウスにおいて，ストレスによる心拍・血圧上昇が減弱することが知られていることから，視床下…

DOI 医中誌

河合 洋幸, 永井 佑茉, 好田 匡志, 西谷 直也, 永安 一樹, 白川 久志, 金子 周司 日本薬理学会年会要旨集 94 (0), 2-Y-F3-1-, 2021

...GCaMP6s, a fluorescent Ca²⁺ indicator, was expressed in MRN serotonin neurons to monitor neuronal activity....

DOI Web Site

高島 大樹, 飯代 智之, 本川 佳幸, 武藤 信一, 相馬 晋司 日本毒性学会学術年会 48.1 (0), P-148-, 2021

...</p><p>【方法】Caセンサータンパク質GCaMP導入hiPSC-CM（マイオリッジ社）を、低吸着プレートを用いてスフェロイド形態で培養した。陽性物質にソタロール、モキシフロキサシン、リドカイン、ベラパミル及びペンタミジンを、陰性物質にアスピリンを用いた。...

DOI

Siew Hoong Yip, Xinhuai Liu, Sabine Hessler, Isaiah Cheong, Robert Porteous, Allan E Herbison Endocrinology 162 (3), 2020-12-26

...Confocal GCaMP6 imaging of GnRH neuron distal dendrons in acute para-horizontal brain slices from GnRH-Cre mice injected with Cre-dependent GCaMP6s adeno-associated viruses demonstrated no effects of 30...

DOI PDF PDF 被引用文献1件

Yugo Miyata, Hiroto Fuse, Shoko Tokumoto, Yusuke Hiki, Ruslan Deviatiiarov, Yuki Yoshida, Takahiro G. Yamada, Richard Cornette, Oleg Gusev, Elena Shagimardanova, Akira Funahashi, Takahiro Kikawada 2020-10-15

...Using the Cas9-mediated gene knock-in system, we established GCaMP3-stably expressing Pv11 cells to monitor intracellular Ca<jats:sup>2+</jats:sup> mobilization....

DOI Web Site 被引用文献1件

Chao J. Liu, Arani Roy, Anthony A. Simons, Deano M. Farinella, Prakash Kara Scientific Reports 10 (1), 16351-, 2020-10-01

...Three-photon imaging of neuronal calcium signals, through the genetically-encoded calcium indicator GCaMP6, has been used to successfully record neuronal activity in deeper neocortical layers and parts...

DOI PDF Web Site 被引用文献1件

MONAI, Hiromu お茶の水女子大学自然科学報告 71 (特別号), 39-50, 2020-09

紀要論文

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Sigita, Augustinaite, Bernd, Kuhn Current Biology 30 (20), 3945-3960.e5, 2020-08-20

Layer 6 (L6) corticothalamic neurons project to thalamus, where they are thought to regulate sensory information transmission to cortex. However, the activity of these neurons during different …

DOI 機関リポジトリ Web Site ほか1件 被引用文献3件

Milena M. Milosevic, Jinyoung Jang, Eric J. McKimm, Mei Hong Zhu, Srdjan D. Antic eneuro 7 (5), ENEURO.0060-20.2020, 2020-08-14

...<jats:p>Genetically encoded voltage indicators (GEVIs) could potentially be used for mapping neural circuits at the plane of synaptic potentials and plateau potentials—two blind spots of GCaMP-based imaging...

DOI Web Site 被引用文献1件

Anthony D Umpierre, Lauren L Bystrom, Yanlu Ying, Yong U Liu, Gregory Worrell, Long-Jun Wu eLife 9 e56502-, 2020-07-27

...Using multiple GCaMP6 variants targeted to microglia, we assessed how microglial calcium signaling responds to alterations in neuronal activity across a wide range....

DOI PDF Web Site ほか1件 被引用文献1件

佐藤, 正晃, 水田, 恒太郎, イスラム, タンビル, 河野, 真子, 関根, 友紀子, 竹川, 高志, ゴメスードミンゲス, ダニエル, シュミット, アレキサンダー, ウォルフ, フレッド, キム, カラム, 山川, 宏, 大倉, 正道, リー, ミン・グー, 深井, 朋樹, 中井, 淳一, 林, 康紀 Cell Reports 32 (1), 107864-, 2020-07-07

In the hippocampus, locations associated with salient features are represented by a disproportionately large number of neurons, but the cellular and molecular mechanisms underlying this …

DOI 機関リポジトリ HANDLE ほか2件 被引用文献8件 参考文献58件

Jane E. Hartung, Michael S. Gold Cell Calcium 89 102225-, 2020-07

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Joseph L. Dynes, Andriy V. Yeromin, Michael D. Cahalan Journal of General Physiology 152 (9), e201812239-, 2020-06-26

...After Ca2+ store depletion in HEK cells cotransfected with mCherry-STIM1 and Orai1-GCaMP6f, Orai1-GCaMP6f fluorescence increased progressively with increasingly negative test potentials and robust responses...

DOI PDF 被引用文献1件

Carlo N. G. Giachello, Aref Arzan Zarin, Hiroshi Kohsaka, Yuen Ngan Fan, Akinao Nose, Matthias Landgraf, Richard A. Baines 2020-06-19

...Thus, validation of connectivity in this CNS has been more reliant on behavioural analyses and, in particular, activity imaging using the calcium-sensor GCaMP....

DOI Web Site 参考文献33件

Natalia V. Barykina, Vladimir P. Sotskov, Anna M. Gruzdeva, You Kure Wu, Ruben Portugues, Oksana M. Subach, Elizaveta S. Chefanova, Viktor V. Plusnin, Olga I. Ivashkina, Konstantin V. Anokhin, Anna V. Vlaskina, Dmitry A. Korzhenevskiy, Alena Y. Nikolaeva, Konstantin M. Boyko, Tatiana V. Rakitina, Anna M. Varizhuk, Galina E. Pozmogova, Fedor V. Subach International Journal of Molecular Sciences 21 (8), 3012-, 2020-04-24

...The ratiometric response of FGCaMP7 is 1.6- and 1.4-fold higher, compared to the intensiometric response of GCaMP6s, in non-stimulated and stimulated neuronal cultures, respectively....

DOI Web Site 被引用文献1件

Shunpei Moriya, Akira Yamashita, Daiki Masukawa, Honami Setoyama, Yunsu Hwang, Akihiro Yamanaka, Tomoyuki Kuwaki Molecular Brain 13 (1), 2020-04-14

...We measured G-CaMP6/mCherry fluorescence intensity in the A13 site to acute nociceptive stimuli (pinch stimulus and heat stimulus)....

DOI PDF Web Site 被引用文献2件 参考文献15件

Nick A. Guagliardo, Peter M. Klein, Christina A. Gancayco, Adam Lu, Sining Leng, Rany R. Makarem, Chelsea Cho, Craig G. Rusin, David T. Breault, Paula Q. Barrett, Mark P. Beenhakker Nature Communications 11 (1), 1679-, 2020-04-03

...To determine if structure informs function, we generated mice with zG-specific expression of GCaMP3 and imaged zG cells within their native rosette structure....

DOI PDF Web Site 被引用文献1件

Fangmiao Sun, Jingheng Zhou, Bing Dai, Tongrui Qian, Jianzhi Zeng, Xuelin Li, Yizhou Zhuo, Yajun Zhang, Ke Tan, Jiesi Feng, Hui Dong, Cheng Qian, Dayu Lin, Guohong Cui, Yulong Li 2020-03-28

...Importantly, co-expressing red GRAB<jats:sub>DA</jats:sub> with either green GRAB<jats:sub>DA</jats:sub> or the calcium indicator GCaMP6s provides a robust tool for simultaneously tracking neuronal activity...

DOI Web Site 被引用文献2件

Patricia Pais-Roldán, Kengo Takahashi, Filip Sobczak, Yi Chen, Xiaoning Zhao, Hang Zeng, Yuanyuan Jiang, Xin Yu Proceedings of the National Academy of Sciences 117 (12), 6875-6882, 2020-03-05

...Here, we complemented resting-state fMRI in rats with cortical calcium recording (GCaMP-mediated) and pupillometry to tackle the linkage between brain-state changes and neural dynamics across different...

DOI Web Site Web Site 被引用文献1件

Kicheon Park, Anuki C. Liyanage, Alan P. Koretsky, Yingtian Pan, Congwu Du Quantitative Imaging in Medicine and Surgery 11 (3), 998-1009, 2020-03

DOI Web Site 被引用文献1件

Joseph Cichon, Jordi Magrané, Elina Shtridler, Chao Chen, Linlin Sun, Guang Yang, Wen-Biao Gan Journal of Neuroscience Methods 334 108535-, 2020-03

DOI Web Site Web Site 被引用文献1件

Oksana M. Subach, Vladimir P. Sotskov, Viktor V. Plusnin, Anna M. Gruzdeva, Natalia V. Barykina, Olga I. Ivashkina, Konstantin V. Anokhin, Alena Y. Nikolaeva, Dmitry A. Korzhenevskiy, Anna V. Vlaskina, Vladimir A. Lazarenko, Konstantin M. Boyko, Tatiana V. Rakitina, Anna M. Varizhuk, Galina E. Pozmogova, Oleg V. Podgorny, Kiryl D. Piatkevich, Edward S. Boyden, Fedor V. Subach International Journal of Molecular Sciences 21 (5), 1644-, 2020-02-28

...NCaMP7 demonstrated a 1.7-fold higher brightness and similar calcium-association/dissociation dynamics compared to the standard GCaMP6s GECI in vitro....

DOI Web Site 被引用文献5件

Shunpei Moriya, Akira Yamashita, Daiki Masukawa, Yuki Kambe, Junichi Sakaguchi, Honami Setoyama, Akihiro Yamanaka, Tomoyuki Kuwaki Molecular Brain 13 (1), 14-, 2020-01-31

...site-specific injection of adeno associated virus (AAV-TetO(3G)-G-CaMP6)....

DOI PDF Web Site 被引用文献4件 参考文献43件

Bernard T. Drumm, Benjamin E. Rembetski, Katelyn Messersmith, Marena S. Manierka, Salah A. Baker, Kenton M. Sanders The Journal of Physiology 598 (4), 651-681, 2020-01-30

...We investigated Ca<jats:sup>2+</jats:sup> signalling in a novel population of ICC that lies along the subserosal surface of LM (ICC‐SS) in mice expressing GCaMP6f in ICC....

DOI Web Site Web Site ほか2件 被引用文献1件

Tianyu Wang, Chunyan Wu, Dimitre G Ouzounov, Wenchao Gu, Fei Xia, Minsu Kim, Xusan Yang, Melissa R Warden, Chris Xu eLife 9 e53205-, 2020-01-30

...We calculated and experimentally verified the excitation pulse energy to achieve the minimum photon count required for the detection of calcium transients in GCaMP6s-expressing neurons for 920 nm two-photon...

DOI PDF Web Site ほか1件 被引用文献1件

Julia Binz-Lotter, Christian Jüngst, Markus M. Rinschen, Sybille Koehler, Peter Zentis, Astrid Schauss, Bernhard Schermer, Thomas Benzing, Matthias J. Hackl Journal of the American Society of Nephrology 31 (3), 532-542, 2020-01-10

...>To study the effects of AngII on podocyte calcium levels <jats:italic toggle="yes">in vivo</jats:italic>, we used intravital microscopy of the kidney in mice expressing the calcium indicator protein GCaMP3...

DOI Web Site 被引用文献1件

M. T. Valley, M. G. Moore, J. Zhuang, N. Mesa, D. Castelli, D. Sullivan, M. Reimers, J. Waters Journal of Neurophysiology 123 (1), 356-366, 2020-01-01

...In awake mice of three transgenic lines, the Spatial Model offers improved separation of hemodynamics and changes in GCaMP fluorescence....

DOI Web Site 被引用文献1件

布施 寛人, 宮田 佑吾, 徳本 翔子, コルネット リシャー, 黄川田 隆洋 低温生物工学会誌 66 (1), 51-54, 2020

...Or47a and Orco from Drosophila melanogaster were cloned and co-expressed in Sf9 cells with a calcium indicator protein GCaMP6f....

DOI Web Site

河合 洋幸, 永井 佑茉, 好田 匡志, 永安 一樹, 白川 久志, 金子 周司 日本薬理学会年会要旨集 93 (0), 1-YIA-28-, 2020

...Fluorescent calcium indicator, GCaMP6s, was expressed in MRN serotonergic neurons, by serotonergic neuron specific viral vectors....

DOI Web Site

布施 寛人, 宮田 佑吾, コルネット リシャー, 黄川田 隆洋 低温生物工学会誌 66 (2), 125-129, 2020

...We established the Ca²⁺ indicator stable expressing Pv11 cell line (Pv11-GCaMP6f). Then, we immobilized Pv11-GCaMP6f with BAM and added the calcium ionophore by using perfusion system....

DOI Web Site

Elias T. Lunsford, Dimitri A. Skandalis, James C. Liao Journal of Neurophysiology 122 (6), 2438-2448, 2019-12-01

...We monitored calcium activity with Tg(elav13:GCaMP6s) fish and found synchronous activity between putative cholinergic efferent neurons and motor neurons....

DOI Web Site 被引用文献1件

Elsa Matthus, Jian Sun, Limin Wang, Madhura G Bhat, Amirah B Mohammad-Sidik, Katie A Wilkins, Nathalie Leblanc-Fournier, Valérie Legué, Bruno Moulia, Gary Stacey, Julia M Davies Annals of Botany 124 (7), 1227-1242, 2019-11-27

...Wild type roots expressing GCaMP3 were used to determine the spatial variation of cytosolic calcium increase in response to extracellular ATP....

DOI PDF 被引用文献1件

Christopher J., Roome, Bernd, Kuhn Neuroscience Research 152 15-24, 2019-11-20

...Interestingly for many future applications, the spectral characteristics of ANNINE dyes allows simultaneous imaging with green indicators, like the genetically encoded calcium indicator GCaMP6....

機関リポジトリ

David B Salkoff, Edward Zagha, Erin McCarthy, David A McCormick Cerebral Cortex 30 (1), 421-437, 2019-11-11

...Here, we recorded wide-field neural activity, as revealed by GCaMP6s, from dorsal cortex while simultaneously monitoring orofacial movements, walking, and arousal (pupil diameter) of head-fixed mice performing...

DOI PDF 被引用文献2件

Sandra Romero, Ariel E Hight, Kameron K Clayton, Jennifer Resnik, Ross S Williamson, Kenneth E Hancock, Daniel B Polley Cerebral Cortex 30 (3), 1603-1622, 2019-10-30

...Here, we performed chronic widefield and two-photon calcium imaging from the ACtx of awake Thy1-GCaMP6s reporter mice....

DOI PDF 被引用文献5件

Xiaofan Zhang, Nathaniel C. Noyes, Jianzhi Zeng, Yulong Li, Ronald L. Davis The Journal of Neuroscience 39 (46), 9164-9172, 2019-09-26

...However,<jats:italic>in vivo</jats:italic>functional imaging studies have failed to detect an early forming memory trace in these neurons as reflected by an enhanced G-CaMP signal in response to presentation...

DOI Web Site 被引用文献1件

Amy M LeMessurier, Keven J Laboy-Juárez, Kathryn McClain, Shilin Chen, Theresa Nguyen, Daniel E Feldman eLife 8 2019-08-16

...We compared whisker map somatotopy in L2/3 and L4 excitatory cells of somatosensory (S1) cortex in normally housed vs. tactile-enriched mice, using GCaMP6s imaging....

DOI PDF Web Site ほか1件 被引用文献1件

Adam S. Charles, Alexander Song, Jeff L. Gauthier, Jonathan W. Pillow, David W. Tank 2019-08-06

...demonstrate improved resilience to motion artifacts in sparsely labeled samples using Bessel beams, increased signal-to-noise ratio and cell-count using low numerical aperture Gaussian beams and nuclear GCaMP...

DOI Web Site 被引用文献1件

Thomas W. Gould, William A. Swope, Dante J. Heredia, Robert D. Corrigan, Terence K. Smith American Journal of Physiology-Gastrointestinal and Liver Physiology 317 (2), G210-G221, 2019-08-01

...Conversely, cholinergic neurons [choline acetyltransferase (ChAT)-positive neurons] expressing GCaMP3 markedly increased their activity during the CMMC....

DOI Web Site 被引用文献1件

Geoffrey Denwood, Andrei Tarasov, Albert Salehi, Elisa Vergari, Reshma Ramracheya, Harumi Takahashi, Viacheslav O. Nikolaev, Susumo Seino, Fiona Gribble, Frank Reimann, Patrik Rorsman, Quan Zhang Journal of General Physiology 151 (9), 2019-07-29

...Simultaneous recordings of electrical activity and [Ca2+]i in δ-cells expressing the genetically encoded Ca2+ indicator GCaMP3 revealed that the majority of glucose-induced [Ca2+]i spikes did not correlate...

DOI PDF 参考文献66件

Martine Eilert‐Olsen, Jarand B. Hjukse, Anna E. Thoren, Wannan Tang, Rune Enger, Vidar Jensen, Klas H. Pettersen, Erlend A. Nagelhus Glia 67 (12), 2399-2409, 2019-07-27

...Acute cortical slices were subjected to artificial cerebrospinal fluid with 20% reduction in osmolarity while GCaMP6f fluorescence was imaged with two‐photon microscopy....

DOI Web Site Web Site 被引用文献1件

David B. Salkoff, Edward Zagha, Erin McCarthy, David A. McCormick 2019-07-22

...Here we recorded wide-field neural activity, as revealed by GCaMP6s, from dorsal cortex while simultaneously monitoring orofacial movements, walking, and arousal (pupil diameter) of head-fixed mice performing...

DOI Web Site 被引用文献1件

Bernard T. Drumm, Sung J. Hwang, Salah A. Baker, Sean M. Ward, Kenton M. Sanders The Journal of Physiology 597 (14), 3587-3617, 2019-06-13

...We examined Ca<jats:sup>2+</jats:sup> handling mechanisms in ICC‐IM of murine proximal colon expressing GCaMP6f in ICC. Several Ca<jats:sup>2+</jats:sup> firing sites were detected in each cell....

DOI Web Site Web Site ほか2件 被引用文献1件

Lucas A. C. Souza, Caleb J. Worker, Wencheng Li, Fatima Trebak, Trevor Watkins, Ariana Julia B. Gayban, Evan Yamasaki, Silvana G. Cooper, Bernard T. Drumm, Yumei Feng American Journal of Physiology-Heart and Circulatory Physiology 316 (6), H1389-H1405, 2019-06-01

...In addition, using the genetically encoded Ca<jats:sup>2+</jats:sup> biosensor GCaMP6 to monitor Ca<jats:sup>2+</jats:sup>-signaling events in the neurons of PVN brain slices, we identified a reduction...

DOI Web Site 被引用文献1件

Tímea Éltes, Miklos Szoboszlay, Katalin Kerti‐Szigeti, Zoltan Nusser The Journal of Physiology 597 (11), 2925-2947, 2019-05-06

...We performed experimental data‐based simulations and found that spike inference error rates using MLspike depend strongly on unitary peak amplitudes and GCaMP6f expression levels....

DOI Web Site Web Site ほか2件 被引用文献1件

Yongming Huang, Mayur J. Patil, Mingwei Yu, Peter Liptak, Bradley J. Undem, Xinzhong Dong, Guobin Wang, Shaoyong Yu Neurogastroenterology & Motility 31 (6), 2019-04-04

...Methods</jats:title><jats:p>Extracellular single‐unit recording and two‐photon nodose neuron imaging were performed, respectively, in ex vivo gastroesophageal‐vagal preparations from wild type and Pirt‐GCaMP6...

DOI Web Site Web Site 被引用文献1件

Tim Indersmitten, Michael J. Schachter, Stephanie Young, Natalie Welty, Stephani Otte, Jonathan J. Nassi, Timothy Lovenberg, Pascal Bonaventure, Ryan M. Wyatt Frontiers in Neuroscience 13 176-, 2019-03-01

DOI Web Site 被引用文献2件

Guinto Mark, Ohta Yasumi, Kawahara Mamiko, Haruta Makito, Noda Toshihiko, Sasagawa Kiyotaka, Tokuda Takashi, Ohta Jun 応用物理学会学術講演会講演予稿集 2019.1 (0), 2569-2569, 2019-02-25

DOI

Gregory L. Futia, Arjun Fontaine, Samuel Littich, Connor McCullough, Diego Restrepo, Richard Weir, John Caldwell, Emily A. Gibson Optogenetics and Optical Manipulation 2019 2019-02-22

DOI 被引用文献1件

Sara C. M. Leijon, Amanda F. Neves, Joseph M. Breza, Sidney A. Simon, Nirupa Chaudhari, Stephen D. Roper The Journal of Physiology 597 (7), 2045-2061, 2019-02-10

...We carried out <jats:italic>in vivo</jats:italic> confocal calcium imaging of trigeminal ganglia in which neurons express GCaMP3 or GCAMP6s and recorded their responses to oral stimulation with thermal...

DOI Web Site Web Site ほか2件 被引用文献1件

森, 誠之, 森, 泰生, Yamauchi, Hajime, Yoshida, Masashi, Mori, X., Masayuki, Hida, Yamato, Tran, Nam, Ha, Ohkura, Masamichi, Abe, Manabu, Akimoto, Yoshihiro, Nakamichi, Yoko, Nishiwaki, Chiyono, Kawakami, Hayato, Hara, Kazuo, Sakimura, Kenji, Nagamatsu, Shinya, Mori, Yasuo, Nakai, Junichi, Kakei, Masafumi, Ohtsuka, Toshihisa Cell Reports 26 (5), 1213-1226.e7, 2019-01-29

...In situ Ca²⁺ imaging of β cells within islets expressing a membrane-bound G-CaMP8b Ca²⁺ sensor demonstrated initial local Ca²⁺ signals at the ELKS-localized vascular side of the β cell plasma membrane,...

HANDLE

Su Young Han, Grace Kane, Isaiah Cheong, Allan E Herbison Endocrinology 160 (3), 557-567, 2019-01-15

DOI PDF 被引用文献8件

Murat Yildirim, Hiroki Sugihara, Peter T. C. So, Mriganka Sur Nature Communications 10 (1), 177-, 2019-01-11

...We image functional visual responses of neurons expressing GCaMP6s across all layers of the primary visual cortex (V1) and in the subplate....

DOI PDF Web Site 被引用文献3件

Sara Mederos, Alicia Hernández‐Vivanco, Jorge Ramírez‐Franco, Mario Martín‐Fernández, Marta Navarrete, Aimei Yang, Edward S. Boyden, Gertrudis Perea Glia 67 (5), 915-934, 2019-01-11

...Using the genetically encoded Ca<jats:sup>2+</jats:sup> indicator GCaMP6f and two‐photon imaging, we show that melanopsin is both competent to stimulate robust IP3‐dependent Ca<jats:sup>2+</jats:sup> signals...

DOI Web Site Web Site ほか1件 被引用文献6件

Xiaojing Lin, Tingbao Zhao, Wenhui Xiong, Shaonan Wen, Xiaoming Jin, Xiao-Ming Xu Journal of Visualized Experiments (143) 2019-01-07

DOI Web Site 被引用文献1件

Jing Liu, Scott C. Baraban eneuro 6 (1), ENEURO.0041-19.2019, 2019-01

...Here, we combine fast confocal imaging of genetically encoded calcium indicator (GCaMP)-expressing larval zebrafish with local field potential (LFP) recordings to study epileptiform events at whole-brain...

DOI Web Site 被引用文献1件

Sharmila Venugopal, Rahul Srinivasan, Baljit S. Khakh Springer Series in Computational Neuroscience 455-470, 2019

DOI Web Site 被引用文献2件

I. Parrón, J. Álvarez, M. Jané, T. Cornejo Sánchez, E. Razquin, S. Guix, G. Camps, C. Pérez, À. Domínguez Epidemiology and Infection 147 2019

<jats:title>Abstract</jats:title><jats:p>On 16 March 2018, a nursing home notified a possible acute gastroenteritis outbreak that affected 11 people. Descriptive and case–control studies and …

DOI Web Site 被引用文献1件

齋藤 瞭毅, 曽我 卓司, 野村 洋, 南 雅文, 人羅 菜津子 日本薬理学会年会要旨集 92 (0), 2-YIA-05-, 2019

...To record ACC-PAG activities from behaving mice, a genetically encoded calcium indicator, GCaMP6, was expressed selectively in the ACC-PAG pathway and its fluorescence was detected through an optic fiber...

DOI Web Site

永安 一樹 日本薬理学雑誌 153 (5), 204-209, 2019

...<p>ウイルスベクターは，神経細胞やグリア細胞などの脳細胞をはじめとする様々な細胞に対して，in vivo/in vitroで外来遺伝子を送達可能な手段として広く用いられている．ChR2やNpHR，DREADDを用いた介入実験や，GCaMPやGECOを用いた神経活動の解析など，光遺伝学／薬理遺伝学的実験が，ごく一般的に行われるようになっているが，その背景には，上記遺伝学的ツールを高いレベルで発現させ...

DOI Web Site Web Site ほか2件 参考文献26件

伊藤 慶, 竹内 遼介, 恩田 正成, 鈴木 俊章, 森本 菜央, 小坂田 文隆 日本薬理学会年会要旨集 92 (0), 1-P-022-, 2019

...Viral vectors encoding the calcium indicator protein GCaMP6m were injected into the visual cortex of adult C57BL/6j mice....

DOI Web Site

人羅(今村) 菜津子 日本薬理学会年会要旨集 92 (0), 2-MS3-, 2019

...The development of genetically encoded calcium indicators, such as GCaMPs, allows us for in vivo visualization of calcium dynamics of specific neuronal populations....

DOI Web Site

Nicholas J. Michelson, James R. Eles, Alberto L. Vazquez, Kip A. Ludwig, Takashi D. Y. Kozai Journal of Neuroscience Research 97 (5), 620-638, 2018-12-26

...In this work, in vivo two‐photon microscopy was used to image neuronal calcium activity in layer 2/3 neurons of somatosensory cortex (S1) in male C57BL/6J‐Tg(Thy1‐GCaMP6s)GP4.3Dkim/J mice during 30 s of...

DOI Web Site Web Site ほか1件 被引用文献1件

Benjamin B. Scott, Stephan Y. Thiberge, Caiying Guo, D. Gowanlock R. Tervo, Carlos D. Brody, Alla Y. Karpova, David W. Tank Neuron 100 (5), 1045-1058.e5, 2018-12

DOI Web Site Web Site 被引用文献2件

David S Greenberg, Damian J Wallace, Kay-Michael Voit, Silvia Wuertenberger, Uwe Czubayko, Arne Monsees, Takashi Handa, Joshua T Vogelstein, Reinhard Seifert, Yvonne Groemping, Jason ND Kerr 2018-11-29

...The model's physical interpretation allowed the same parameters to describe GCaMP6s binding kinetics for both in vitro binding assays and in vivo imaging....

DOI Web Site 被引用文献1件

Natalia V. Barykina, Danila A. Doronin, Oksana M. Subach, Vladimir P. Sotskov, Viktor V. Plusnin, Olga A. Ivleva, Anna M. Gruzdeva, Tatiana A. Kunitsyna, Olga I. Ivashkina, Alexander A. Lazutkin, Aleksey Y. Malyshev, Ivan V. Smirnov, Anna M. Varizhuk, Galina E. Pozmogova, Kiryl D. Piatkevich, Konstantin V. Anokhin, Grigori Enikolopov, Fedor V. Subach Scientific Reports 8 (1), 2018-10-15

...On patched neurons, YTnC had a 2.6-fold lower ΔF/F than GCaMP6s....

DOI PDF Web Site 被引用文献1件

Hod Dana, Yi Sun, Boaz Mohar, Brad Hulse, Jeremy P. Hasseman, Getahun Tsegaye, Arthur Tsang, Allan Wong, Ronak Patel, John J. Macklin, Yang Chen, Arthur Konnerth, Vivek Jayaraman, Loren L. Looger, Eric R. Schreiter, Karel Svoboda, Douglas S. Kim 2018-10-03

...Using structure-guided mutagenesis and neuron-based screening, we optimized the green fluorescent protein-based GECI GCaMP6 for different modes of <jats:italic>in vivo</jats:italic> imaging....

DOI Web Site 被引用文献1件

Kristen E. Severi, Urs L. Böhm, Claire Wyart Scientific Reports 8 (1), 2018-09-11

...Here, we mapped neuronal activity with single-cell resolution in the hindbrain of restrained transgenic <jats:italic>Tg(HuC:GCaMP5G)</jats:italic> zebrafish larvae swimming in response to whole-field visual...

DOI PDF Web Site 被引用文献1件

Yu-Wei Wu, Susan Gordleeva, Xiaofang Tang, Pei-Yu Shih, Yulia Dembitskaya, Alexey Semyanov 2018-09-06

...Here we identify the initiation points of the Ca<jats:sup>2+</jats:sup> events within the territory of single astrocytes expressing genetically encoded Ca<jats:sup>2+</jats:sup> indicator GCaMP2 in culture...

DOI Web Site 被引用文献1件

Alicia Sampieri, Karla Santoyo, Alexander Asanov, Luis Vaca Scientific Reports 8 (1), 2018-09-05

...distributed in the ER lumen (ER-GCaMP)....

DOI PDF Web Site 被引用文献1件

Yong Qian, Vladimir Rancic, Jiahui Wu, Klaus Ballanyi, Robert E. Campbell ChemBioChem 20 (4), 516-520, 2018-07-31

...One drawback of green fluorescent GECIs, such as the widely used GCaMP6, is that the blue wavelengths of light used to excite the GECI also activate optogenetic actuators such as channelrhodopsins....

DOI Web Site Web Site ほか1件 被引用文献8件

Richard C. Rogers, David H. McDougal, Sue Ritter, Emily Qualls-Creekmore, Gerlinda E. Hermann American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 315 (1), R153-R164, 2018-07-01

...tyrosine hydroxylase neurons (TH-GCaMP5)....

DOI Web Site 被引用文献1件

Alexander D. Jacob, Adam I. Ramsaran, Andrew J. Mocle, Lina M. Tran, Chen Yan, Paul W. Frankland, Sheena A. Josselyn Current Protocols in Neuroscience 84 (1), 2018-06-26

...The CHEndoscope uses an implanted gradient index lens along with the genetically encoded calcium indicator GCaMP6 to image calcium transients from hundreds of neurons simultaneously in awake behaving mice...

DOI Web Site Web Site 被引用文献1件

Nicholas J. Michelson, James R. Eles, Alberto L. Vazquez, Kip A Ludwig, Takashi DY Kozai 2018-06-07

...In this work, <jats:italic>in vivo</jats:italic> two-photon microscopy was used to image neuronal calcium activity in layer 2/3 neurons of somatosensory cortex (S1) in male C57BL/6J-Tg(Thy1-GCaMP6s)GP4.3Dkim...

DOI Web Site 被引用文献1件

Eriko Yoshida, Shin-Ichiro Terada, Yasuyo H. Tanaka, Kenta Kobayashi, Masamichi Ohkura, Junichi Nakai, Masanori Matsuzaki Scientific Reports 8 (1), 8324-, 2018-05-29

...When we imaged motor cortical layer 1 in a behaving head-restrained mouse, calcium transients were detected in presynaptic boutons of thalamocortical axons sparsely labeled with GCaMP6s, although their...

DOI PDF Web Site 被引用文献2件 参考文献53件

Baris N. Ozbay, Gregory L. Futia, Ming Ma, Victor M. Bright, Juliet T. Gopinath, Ethan G. Hughes, Diego Restrepo, Emily A. Gibson Scientific Reports 8 (1), 2018-05-25

...We show three-dimensional two-photon imaging of neuronal structure and record neuronal activity from GCaMP6s fluorescence from multiple focal planes in a freely-moving mouse....

DOI PDF Web Site 被引用文献1件

Yaxiong Yang, Nan Liu, Yuanyuan He, Yuxia Liu, Lin Ge, Linzhi Zou, Sen Song, Wei Xiong, Xiaodong Liu Nature Communications 9 (1), 2018-04-17

...In summary, CaM/Ca<jats:sub>V</jats:sub>1 gating and signaling mechanisms are elucidated for GCaMP side-effects, while allowing the development of GCaMP-X to appropriately monitor cytosolic, submembrane...

DOI PDF Web Site 被引用文献3件

Bernard T. Drumm, Benjamin E. Rembetski, Caroline A. Cobine, Salah A. Baker, Gerard P. Sergeant, Mark A. Hollywood, Keith D. Thornbury, Kenton M. Sanders The Journal of Physiology 596 (8), 1433-1466, 2018-04-15

...Ca<jats:sup>2+</jats:sup> signalling in USMCs was investigated in intact urethral muscles using a genetically encoded Ca<jats:sup>2+</jats:sup> sensor, GCaMP3, expressed selectively in USMCs....

DOI Web Site Web Site 被引用文献1件

Yoshihisa Yamada, Shohei Wakao, Yoshihiro Kushida, Shingo Minatoguchi, Atsushi Mikami, Kenshi Higashi, Shinya Baba, Taeko Shigemoto, Yasumasa Kuroda, Hiromitsu Kanamori, Mohamad Amin, Masanori Kawasaki, Kazuhiko Nishigaki, Masato Taoka, Toshiaki Isobe, Chisako Muramatsu, Mari Dezawa, Shinya Minatoguchi Circulation Research 122 (8), 1069-1083, 2018-04-13

...GCaMP3 (GFP-based Ca calmodulin probe)-labeled Muse cells that engrafted into the ischemic region exhibited increased GCaMP3 fluorescence during systole and decreased fluorescence during diastole....

DOI Web Site 被引用文献30件 参考文献39件

Salah A. Baker, Bernard T. Drumm, Karolina E. Skowronek, Benjamin E. Rembetski, Lauren E. Peri, Grant W. Hennig, Brian A. Perrino, Kenton M. Sanders eneuro 5 (2), ENEURO.0080-18.2018, 2018-03

...High-resolution confocal microscopy was used to image Ca<jats:sup>2+</jats:sup>transients in ICC-DMP within murine small intestinal muscles with cell-specific expression of GCaMP3....

DOI Web Site 被引用文献1件

Takahiro Futatsuki, Akira Yamashita, Khairunnisa Novita Ikbar, Akihiro Yamanaka, Kazunori Arita, Yasuyuki Kakihana, Tomoyuki Kuwaki Scientific Reports 8 (1), 2717-, 2018-02-09

...Orexin neurons were active just before onset of hypothermia and during the recovery period as revealed by calcium imaging <jats:italic>in vivo</jats:italic> using G-CaMP....

DOI PDF Web Site 被引用文献8件 参考文献43件

Maosen Wang, Yi He, Terrence J. Sejnowski, Xin Yu Proceedings of the National Academy of Sciences 115 (7), 2018-01-30

...Here, we applied simultaneous fMRI and GCaMP-mediated Ca <jats:sup>2+</jats:sup> optical fiber recording to detect distinct astrocytic Ca <jats:sup>2+</jats:sup>...

DOI Web Site Web Site 被引用文献1件

Eiji Shigetomi, Yukiho J. Hirayama, Kazuhiro Ikenaka, Kenji F. Tanaka, Schuichi Koizumi The Journal of Neuroscience 38 (6), 1383-1395, 2018-01-05

...signals, we engineered mice (both sexes) to specifically overexpress P2Y1Rs in astrocytes, and we visualized Ca<jats:sup>2+</jats:sup>signals via a genetically encoded Ca<jats:sup>2+</jats:sup>indicator, GCaMP6f...

DOI Web Site 被引用文献7件 参考文献57件

Lauren A. Biwer, Miranda E. Good, Kwangseok Hong, Rahul K. Patel, Neha Agrawal, Robin Looft-Wilson, Swapnil K. Sonkusare, Brant E. Isakson Arteriosclerosis, Thrombosis, and Vascular Biology 38 (1), 120-130, 2018-01

...Comparison of ER calcium in arteries and use of an ER-specific GCaMP indicator in vitro revealed no observable difference in ER calcium with Calr knockout....

DOI Web Site 被引用文献1件

Kim I. Chisholm, Nikita Khovanov, Douglas M. Lopes, Federica La Russa, Stephen B. McMahon eneuro 5 (1), ENEURO.0417-17.2018, 2018-01

...To assess large networks of sensory neurons, we selectively label primary afferents with GCaMP6s in male and female C57bl/6 mice and visualize their functional responses to peripheral stimulation<jats:...

DOI Web Site 被引用文献1件

Andrew E. Hudson Methods in Enzymology 603 197-220, 2018

DOI Web Site Web Site 被引用文献2件

Xiaomin Xing, Chun-Fang Wu eneuro 5 (1), ENEURO.0362-17.2018, 2018-01

...of presynaptic GCaMP signals from in situ imaging studies....

DOI Web Site 被引用文献1件

Hitora-Imamura Natsuko, Soga Takuji, Minami Masabumi 日本薬理学会年会要旨集 WCP2018 (0), PO2-1-73-, 2018

...GCaMP6 fluorescence was monitored through optic fibers implanted into the brain....

DOI Web Site

McMahon Stephen B. 日本薬理学会年会要旨集 WCP2018 (0), CL-3-, 2018

...Here I will review our recent work studying populations of these cells again using GCaMP as a reporter....

DOI Web Site

Sano Fumikazu, Shigetomi Eiji, Koizumi Schuichi, Kanemura Hideaki, Mikoshiba Katsuhiko, Aihara Masao 日本薬理学会年会要旨集 WCP2018 (0), OR24-2-, 2018

...For functional analysis of astrocytes, Ca2+ imaging were performed from hippocampal slices 4 weeks after SE, using either GCaMP3 or Fluo-4AM....

DOI Web Site

Shigetomi Eiji, Hirayama Yukiho J, Ikenaka Kazuhiro, Tanaka Kenji F, Bito Haruhiko, Koizumi Schuichi 日本薬理学会年会要旨集 WCP2018 (0), PO1-1-105-, 2018

...</p><p>Conclusion: Dual color Ca²⁺ imaging using R-CaMP2 and GCaMP6f revealed spatiotemporal dynamics of dendritic Ca²⁺ and astrocytic process Ca²⁺....

DOI Web Site

Tobias X Dong, Shivashankar Othy, Amit Jairaman, Jonathan Skupsky, Angel Zavala, Ian Parker, Joseph L Dynes, Michael D Cahalan eLife 6 e32417-, 2017-12-14

...Here, we describe development and characterization of ‘Salsa6f’, a fusion of GCaMP6f and tdTomato optimized for cell tracking while monitoring cytosolic Ca2+, and a transgenic Ca2+ reporter mouse with...

DOI PDF Web Site ほか1件 被引用文献2件

木矢 星歌, 櫻井 武, 岩見 雅史, 木矢 剛智, Hara Chiho, Morishita Koudai, Takayanagi-Kiya Seika, Mikami Akihisa, Uchino Keiro, Sakurai Takeshi, Kanzaki Ryohei, Sezutsu Hideki, Iwami Masafumi, Kiya Taketoshi Scientific Reports 7 (1), 11795-, 2017-12-01

...Enhanced protein expression of membrane-targeted GFP and calcium indicator protein (GCaMP5G) drastically improved visualization of fine neurite structures and neural activity, respectively....

DOI 機関リポジトリ HANDLE

大倉 正道, 中井 淳一 生体の科学 68 (5), 442-443, 2017-10-15

DOI

Jiakun Chen, Li Xia, Michael R. Bruchas, Lilianna Solnica-Krezel Developmental Biology 430 (2), 385-396, 2017-10

DOI Web Site Web Site 被引用文献1件

Fang Guo, Xiao Chen, Michael Rosbash Proceedings of the National Academy of Sciences 114 (41), E8780-, 2017-09-26

...This new approach is complementary to electrophysiological recording and GCaMP imaging, especially for small organisms and behavorial paradigms for which these more traditional methods are not practical...

DOI Web Site 被引用文献5件

Koji Matsuda, Masayuki Yoshida, Koichi Kawakami, Masahiko Hibi, Takashi Shimizu Scientific Reports 7 (1), 11865-, 2017-09-19

...To investigate the roles of the zebrafish cerebellum in classical fear conditioning, we expressed botulinum toxin or the Ca<jats:sup>2+</jats:sup> indicator GCaMP7a in cerebellar neurons....

DOI PDF Web Site 被引用文献4件 参考文献46件

Chiho Hara, Koudai Morishita, Seika Takayanagi-Kiya, Akihisa Mikami, Keiro Uchino, Takeshi Sakurai, Ryohei Kanzaki, Hideki Sezutsu, Masafumi Iwami, Taketoshi Kiya Scientific Reports 7 (1), 11795-, 2017-09-18

...Enhanced protein expression of membrane-targeted GFP and calcium indicator protein (GCaMP5G) drastically improved visualization of fine neurite structures and neural activity, respectively....

DOI PDF Web Site 被引用文献3件 参考文献49件

Cindy Ast, Jessica Foret, Luke M. Oltrogge, Roberto De Michele, Thomas J. Kleist, Cheng-Hsun Ho, Wolf B. Frommer Nature Communications 8 (1), 431-, 2017-09-05

...We additionally identified the internal acid-base equilibrium as a key determinant of the GCaMP dynamic range....

DOI PDF Web Site 被引用文献5件

Nicholas A. Steinmetz, Christina Buetfering, Jerome Lecoq, Christian R. Lee, Andrew J. Peters, Elina A. K. Jacobs, Philip Coen, Douglas R. Ollerenshaw, Matthew T. Valley, Saskia E. J. de Vries, Marina Garrett, Jun Zhuang, Peter A. Groblewski, Sahar Manavi, Jesse Miles, Casey White, Eric Lee, Fiona Griffin, Joshua D. Larkin, Kate Roll, Sissy Cross, Thuyanh V. Nguyen, Rachael Larsen, Julie Pendergraft, Tanya Daigle, Bosiljka Tasic, Carol L. Thompson, Jack Waters, Shawn Olsen, David J. Margolis, Hongkui Zeng, Michael Hausser, Matteo Carandini, Kenneth D. Harris eneuro 4 (5), ENEURO.0207-17.2017, 2017-09

...Here we report aberrant electrical activity, similar to interictal spikes, and accompanying fluorescence events in some genotypes of transgenic mice expressing GCaMP6 genetically encoded calcium sensors...

DOI Web Site 被引用文献7件

Annie Liu, Nathaniel N. Urban eneuro 4 (5), ENEURO.0129-17.2017, 2017-09

...We use viral expression of GCaMP6s to examine odor-evoked responses of MCs following prenatal and early postnatal odorant exposure to two dissimilar odorants, methyl salicylate (MS) and hexanal, which...

DOI Web Site 被引用文献4件

Dominique Förster, Marco Dal Maschio, Eva Laurell, Herwig Baier Nature Communications 8 (1), 116-, 2017-07-24

...By combining the Gal4-UAS system with Cre/lox recombination, we target the optogenetic actuator ChrimsonR and the sensor GCaMP6 to stochastically labeled, nonoverlapping and sparse subsets of neurons....

DOI PDF Web Site 被引用文献3件

Takako Noguchi, Tanya L. Leise, Nathaniel J. Kingsbury, Tanja Diemer, Lexie L. Wang, Michael A. Henson, David K. Welsh eneuro 4 (4), ENEURO.0160-17.2017, 2017-07

...expression and [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>rhythms in the SCN, as well as the SCN neuronal network dependence of [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub>rhythms, we introduced GCaMP3...

DOI Web Site 被引用文献7件

L. Turrini, C. Fornetto, G. Marchetto, M. C. Müllenbroich, N. Tiso, A. Vettori, F. Resta, A. Masi, G. Mannaioni, F. S. Pavone, F. Vanzi Scientific Reports 7 (1), 3025-, 2017-06-08

...We also present simultaneous measurements of brain and locomotor activity, as well as a high-throughput assay, demonstrating that GCaMP measurements can complement behavioural assays for the detection...

DOI PDF Web Site 被引用文献2件

Bernard T. Drumm, Grant W. Hennig, Matthew J. Battersby, Erin K. Cunningham, Tae Sik Sung, Sean M. Ward, Kenton M. Sanders, Salah A. Baker Journal of General Physiology 149 (7), 703-725, 2017-06-07

...Here, we characterize the nature of Ca2+ transients in ICC-MY within intact muscles, using mice expressing a genetically encoded Ca2+ sensor, GCaMP3, in ICC....

DOI PDF 被引用文献1件

Thomas A. DeFalco, Masatsugu Toyota, Van Phan, Purva Karia, Wolfgang Moeder, Simon Gilroy, Keiko Yoshioka Plant and Cell Physiology 58 (7), 1173-1184, 2017-05-08

DOI PDF 被引用文献6件 参考文献73件

Ono, Daisuke, Honma, Sato, Nakajima, Yoshihiro, Kuroda, Shigeru, Enoki, Ryosuke, Honma, Ken-ichi Proceedings of the National Academy of Sciences of the United States of America 114 (18), E3699-E3708, 2017-05-02

...Furthermore, neither is synchronized with circadian intracellular Ca^2+ rhythms monitored by a calcium indicator, GCaMP6s, or with firing rhythms monitored on a multielectrode array dish, although the...

機関リポジトリ HANDLE

Reza Ghaemi, Pouya Rezai, Fatemeh Rafiei Nejad, Ponnambalam Ravi Selvaganapathy Biomicrofluidics 11 (3), 34113-, 2017-05-01

...The clamping and immobilization processes were characterized by imaging and movement measurement of the CNS through the expression of genetically encoded Calcium sensor GCaMP5 in all sensory and cholinergic...

DOI PDF 被引用文献1件

Damian Droste, Gerald Seifert, Laura Seddar, Oliver Jädtke, Christian Steinhäuser, Christian Lohr Scientific Reports 7 (1), 2017-03-21

...Here, we investigated AMPA-evoked Ca<jats:sup>2+</jats:sup> influx into olfactory bulb astrocytes in mouse brain slices using Fluo-4 and GCaMP6s, respectively....

DOI PDF Web Site 被引用文献1件

Xiao Chen, Michael Rosbash Nature Communications 8 (1), 2017-03-09

...<jats:italic>In vivo</jats:italic> calcium monitoring, dynamics of PDF projections, ArcLight, GCaMP6 imaging and sleep assays indicate that mir-92a suppresses neuronal excitability....

DOI PDF Web Site 被引用文献1件

Jung-Hwa Cho, Carter J. Swanson, Jeannie Chen, Ang Li, Lisa G. Lippert, Shannon E. Boye, Kasey Rose, Sivaraj Sivaramakrishnan, Cheng-Ming Chuong, Robert H. Chow ACS Chemical Biology 12 (4), 1066-1074, 2017-03-01

DOI Web Site 被引用文献1件

Karl J. Iremonger, Robert Porteous, Allan E. Herbison The Journal of Neuroscience 37 (12), 3342-3351, 2017-02-24

...Here we use GCaMP Ca<jats:sup>2+</jats:sup>imaging in brain slices from mice to address how nerve terminal Ca<jats:sup>2+</jats:sup>is controlled in gonadotropin-releasing hormone (GnRH) neurons via action...

DOI Web Site 被引用文献4件

Dimitre G Ouzounov, Tianyu Wang, Mengran Wang, Danielle D Feng, Nicholas G Horton, Jean C Cruz-Hernández, Yu-Ting Cheng, Jacob Reimer, Andreas S Tolias, Nozomi Nishimura, Chris Xu Nature Methods 14 (4), 388-390, 2017-02-20

DOI PDF Web Site 被引用文献11件

Lauren M. Barnett, Thomas E. Hughes, Mikhail Drobizhev PLOS ONE 12 (2), e0170934-, 2017-02-09

DOI Web Site 被引用文献11件

Jun Zhuang, Lydia Ng, Derric Williams, Matthew Valley, Yang Li, Marina Garrett, Jack Waters eLife 6 1-, 2017-01-06

...We employed fluorescence imaging and GCaMP6 reporter mice to generate retinotopic maps, revealing additional regions of retinotopic organization that extend into barrel and retrosplenial cortices....

DOI PDF Web Site ほか1件 被引用文献2件

Serena Bovetti, Claudio Moretti, Stefano Zucca, Marco Dal Maschio, Paolo Bonifazi, Tommaso Fellin Scientific Reports 7 (1), 40041-, 2017-01-05

...Here, we developed a technique based on patterned two-photon illumination to allow fast scanless imaging of GCaMP6 signals in the intact mouse brain at the same time as single-photon optogenetic inhibition...

DOI PDF Web Site 被引用文献2件

工藤 佳久 歯科薬物療法 36 (1), 1-8, 2017

...Moreover, the molecular biological technique established new Ca²⁺ indicators called GCaMP family by modifying green fluorescence protein....

DOI 医中誌

須永 圭紀, 白石 愛香里, 山口 貴大, 春田 牧人, 野田 俊彦, 笹川 清隆, 徳田 崇, 吉村 由美子, 太田 淳 映像情報メディア学会技術報告 41.19 (0), 1-3, 2017

...本研究では自由行動下マウスに対しGCaMP蛍光イメージングを行うことを目的とした，マウス脳内に直接埋め込み可能な埋植型CMOSイメージングデバイスを開発した．...

DOI Web Site

Nordine Helassa, Borbala Podor, Alan Fine, Katalin Török Scientific Reports 6 (1), 38276-, 2016-12-06

...Here we report the development of reduced affinity ultrafast variants of GCaMP6s and GCaMP6f....

DOI PDF Web Site 被引用文献1件

Anne C. Wolfes, Saheeb Ahmed, Ankit Awasthi, Markus A. Stahlberg, Ashish Rajput, Daniel S. Magruder, Stefan Bonn, Camin Dean Journal of General Physiology 149 (1), 149-170, 2016-12-01

...other monocultures in terms of astrocyte morphology, mRNA expression profile, protein expression of cell maturity markers, and Ca2+ fluctuations: In astrocytes transduced with GFAP promoter–driven Lck-GCaMP3...

DOI PDF 被引用文献1件

Edward C. Emery, Ana P. Luiz, Shafaq Sikandar, Rán Magnúsdóttir, Xinzhong Dong, John N. Wood Science Advances 2 (11), e1600990-, 2016-11-04

<jats:p>In vivo imaging shows that the great majority of somatosensory neurons are modality-specific.</jats:p>

DOI Web Site 被引用文献1件

Ruizhen Li, Julie Leblanc, Kevin He, X. Johné Liu Molecular Biology of the Cell 27 (21), 3273-3283, 2016-11

...Using two calcium probes containing permutated GFP and the calcium sensor calmodulin (Lck-GCaMP3 and GCaMP3), we demonstrated enrichment of the probes at the spindle but failed to detect calcium increase...

DOI 被引用文献1件

Jung Eun Park, Xian Feng Zhang, Sang-Ho Choi, Junko Okahara, Erika Sasaki, Afonso C. Silva Scientific Reports 6 (1), 34931-, 2016-10-11

...Eight transgenic animals were born and shown to have stable and functional GCaMP expression in several different tissues....

DOI PDF Web Site 被引用文献11件

Natalia V. Barykina, Oksana M. Subach, Danila A. Doronin, Vladimir P. Sotskov, Marina A. Roshchina, Tatiana A. Kunitsyna, Aleksey Y. Malyshev, Ivan V. Smirnov, Asya M. Azieva, Ilya S. Sokolov, Kiryl D. Piatkevich, Mikhail S. Burtsev, Anna M. Varizhuk, Galina E. Pozmogova, Konstantin V. Anokhin, Fedor V. Subach, Grigori N. Enikolopov Scientific Reports 6 (1), 34447-, 2016-09-28

...Monomeric NTnC has higher brightness and pH-stability <jats:italic>in vitro</jats:italic> compared with the standard GECI GCaMP6s....

DOI PDF Web Site 被引用文献3件

X Luís Deán-Ben, Gali Sela, Antonella Lauri, Moritz Kneipp, Vasilis Ntziachristos, Gil G Westmeyer, Shy Shoham, Daniel Razansky Light: Science & Applications 5 (12), e16201-e16201, 2016-08-15

...Experiments performed in immobilized and freely swimming larvae and in adult zebrafish brains expressing the genetically encoded calcium indicator GCaMP5G demonstrate, for the first time, the fundamental...

DOI PDF Web Site 被引用文献2件

Christopher A Baker, Yishai M Elyada, Andres Parra, M McLean Bolton eLife 5 1-, 2016-08-15

...using the temporal focusing method and restricting channelrhodopsin to the soma and proximal dendrites, we are able to reliably evoke action potentials in individual neurons, verify spike generation with GCaMP6s...

DOI PDF Web Site ほか1件 被引用文献3件

Eyal Seidemann, Yuzhi Chen, Yoon Bai, Spencer C Chen, Preeti Mehta, Bridget L Kajs, Wilson S Geisler, Boris V Zemelman eLife 5 1-, 2016-07-21

...The measured tuning properties of the GCaMP signals to stimulus contrast, orientation and spatial position closely match the predictions of the model, suggesting that widefield GCaMP signals are linearly...

DOI PDF Web Site ほか1件 被引用文献3件

Joseph B. Wekselblatt, Erik D. Flister, Denise M. Piscopo, Cristopher M. Niell Journal of Neurophysiology 115 (6), 2852-2866, 2016-06-01

...activity at this global scale, we have improved and integrated tools to perform functional imaging across large areas of cortex using a transgenic mouse expressing the genetically encoded calcium sensor GCaMP6s...

DOI Web Site 被引用文献3件

Hod Dana, Boaz Mohar, Yi Sun, Sujatha Narayan, Andrew Gordus, Jeremy P Hasseman, Getahun Tsegaye, Graham T Holt, Amy Hu, Deepika Walpita, Ronak Patel, John J Macklin, Cornelia I Bargmann, Misha B Ahrens, Eric R Schreiter, Vivek Jayaraman, Loren L Looger, Karel Svoboda, Douglas S Kim eLife 5 413-, 2016-03-24

...Here we present improved red GECIs based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a), with sensitivity comparable to GCaMP6....

DOI PDF Web Site ほか1件 被引用文献30件

Hiromu Monai, Masamichi Ohkura, Mika Tanaka, Yuki Oe, Ayumu Konno, Hirokazu Hirai, Katsuhiko Mikoshiba, Shigeyoshi Itohara, Junichi Nakai, Youichi Iwai, Hajime Hirase Nature Communications 7 (1), 11100-, 2016-03-22

...Using a transgenic mouse expressing G-CaMP7 in astrocytes and a subpopulation of excitatory neurons, we find that tDCS induces large-amplitude astrocytic Ca<jats:sup>2+</jats:sup> surges across the entire...

DOI PDF Web Site 被引用文献50件 参考文献46件

Yicheng Xie, Allen W. Chan, Alexander McGirr, Songchao Xue, Dongsheng Xiao, Hongkui Zeng, Timothy H. Murphy The Journal of Neuroscience 36 (4), 1261-1272, 2016-01-27

...Regional cortical connectivity detected by iGluSnFR in spontaneous brain activity identified functional circuits consistent with maps generated from GCaMP3 mice, GCaMP6s mice, or VSD sensors....

DOI Web Site 被引用文献4件

武田 寛史, 星川 椋, 田桑 弘之, 季 斌, 樋口 真人, 正本 和人 バイオエンジニアリング講演会講演論文集 2016.28 (0), _1C21-1_-_1C21-4_, 2016

...The cells expressed calcium-sensitive genetic sensor (GCaMP3) under neural promoter of CaMKII, were also labeled with sulforhodamine 101 (SR101), a marker of glial cells....

DOI Web Site

Sadakane, Osamu, Masamizu, Yoshito, Watakabe, Akiya, Terada, Shin Ichiro, Ohtsuka, Masanari, Takaji, Masafumi, Mizukami, Hiroaki, Ozawa, Keiya, Kawasaki, Hiroshi, Matsuzaki, Masanori, Yamamori, Tetsuo Cell Reports 13 (9), -1989, 2015-12-01

...We used a tetracycline-inducible expression system to robustly amplify neuronal GCaMP6f expression and up- and downregulate it for more than 100days....

DOI HANDLE Web Site ほか1件 被引用文献21件 参考文献56件

Nordine Helassa, Xiao-hua Zhang, Ianina Conte, John Scaringi, Elric Esposito, Jonathan Bradley, Thomas Carter, David Ogden, Martin Morad, Katalin Török Scientific Reports 5 (1), 15978-, 2015-11-03

...At 37 °C, the GCaMP3-derived probe termed GCaMP3<jats:sub><jats:italic>fast</jats:italic></jats:sub> is 40-fold faster than GCaMP3 with Ca<jats:sup>2+</jats:sup> decay and rise times, <jats:italic>t</jats...

DOI PDF Web Site 被引用文献3件

Rachel E. Miller, Abdelhak Belmadani, Shingo Ishihara, Phuong B. Tran, Dongjun Ren, Richard J. Miller, Anne‐Marie Malfait Arthritis & Rheumatology 67 (11), 2933-2943, 2015-10-28

...The [Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub> in neurons within ex vivo intact DRGs was measured in samples from Pirt‐GCaMP3 mice....

DOI Web Site Web Site 被引用文献2件

Mark J. Henderson, Heather A. Baldwin, Christopher A. Werley, Stefano Boccardo, Leslie R. Whitaker, Xiaokang Yan, Graham T. Holt, Eric R. Schreiter, Loren L. Looger, Adam E. Cohen, Douglas S. Kim, Brandon K. Harvey PLOS ONE 10 (10), e0139273-, 2015-10-09

DOI Web Site 被引用文献4件

H. Cagnon, T. Vidal, A. Sellier, X. Bourbon, G. Camps Cement and Concrete Research 76 91-97, 2015-10

DOI Web Site Web Site 被引用文献1件

Minrong Ai, Holly Mills, Makoto Kanai, Jason Lai, Jingjing Deng, Eric Schreiter, Loren Looger, Thomas Neubert, Greg Suh PLOS ONE 10 (9), e0138127-, 2015-09-18

DOI Web Site 被引用文献1件

An Wu, Gennady Dvoryanchikov, Elizabeth Pereira, Nirupa Chaudhari, Stephen D. Roper Nature Communications 6 (1), 8171-, 2015-09-16

...We used mice expressing GCaMP3 in geniculate ganglion sensory neurons to investigate taste-evoked activity....

DOI PDF Web Site 被引用文献2件

Peifu Tang, Yiling Zhang, Chao Chen, Xinran Ji, Furong Ju, Xingyu Liu, Wen-Biao Gan, Zhigang He, Shengxiang Zhang, Wei Li, Lihai Zhang Scientific Reports 5 (1), 9691-, 2015-05-19

...YFP H-line and Thy1-GCaMP transgenic mice were used in thisstudy. Two-photon microscopy was used for imaging of axonal dieback, blood flow, andcalcium influx post-injury....

DOI PDF Web Site 被引用文献1件

Douglas A. Storace, Oliver R. Braubach, Lei Jin, Lawrence B. Cohen, Uhna Sung Scientific Reports 5 (1), 10212-, 2015-05-13

...Comparisons between ArcLight and the protein calcium sensors GCaMP3 and GCaMP6f revealed that ArcLight had faster temporal kinetics that more clearly distinguished activity elicited by individual odorant...

DOI PDF Web Site 被引用文献4件

Masaaki Sato, Masako Kawano, Masamichi Ohkura, Keiko Gengyo-Ando, Junichi Nakai, Yasunori Hayashi PLOS ONE 10 (5), e0125354-, 2015-05-06

DOI Web Site 被引用文献14件 参考文献27件

Richard Piet, Antoine Fraissenon, Ulrich Boehm, Allan E. Herbison The Journal of Neuroscience 35 (17), 6881-6892, 2015-04-29

...Experiments in mice expressing the calcium indicator GCaMP3 in kisspeptin neurons enabled simultaneous monitoring of intracellular calcium concentrations ([Ca<jats:sup>2+</jats:sup>]<jats:sub>i</jats:sub...

DOI Web Site 被引用文献2件

Wannan Tang, Karolina Szokol, Vidar Jensen, Rune Enger, Chintan A. Trivedi, Øivind Hvalby, P. Johannes Helm, Loren L. Looger, Rolf Sprengel, Erlend A. Nagelhus The Journal of Neuroscience 35 (7), 3016-3021, 2015-02-18

...We stimulated the Schaffer collateral/commissural fibers in acute hippocampal slices from adult mice transduced with the genetically encoded Ca<jats:sup>2+</jats:sup>indicator GCaMP5E driven by the glial...

DOI Web Site 被引用文献6件

Lauran Madden, Mark Juhas, William E Kraus, George A Truskey, Nenad Bursac eLife 4 e04885;-, 2015-01-09

...Positive correlation between contractile force and GCaMP6-reported calcium responses enables non-invasive tracking of myobundle function and drug response....

DOI PDF Web Site ほか2件 被引用文献11件

Matteo Carandini, Daisuke Shimaoka, L. Federico Rossi, Tatsuo K. Sato, Andrea Benucci, Thomas Knöpfel The Journal of Neuroscience 35 (1), 53-63, 2015-01-07

...We compared this voltage signal with calcium signals imaged in transgenic mice that express a calcium indicator (GCaMP3) throughout cortex....

DOI Web Site 被引用文献9件

小泉 修一, 平山 友里, 繁冨 英治 日本生物学的精神医学会誌 26 (3), 129-133, 2015

グリア細胞は，神経細胞とは時空間を異なる活動性により脳機能をダイナミックに制御している。グリア細胞，特にアストロサイトは，アセンブリを形成し，複雑・微細な突起を介して神経細胞およびグリア細胞を制御している。これまでの手法では，微細突起部位の機能は不明のままであったが，繁冨らの膜移行型 Ca2 ＋プローブにより，これらの活動が可視化できるようになった。本手法を用いることにより，「虚血耐性」がアスト…

DOI Web Site 医中誌

民山 浩輔, 清水 正宏, 宮坂 恒太, 小椋 利彦, 中井 淳一, 大倉 正道, 細田 耕 ロボティクス・メカトロニクス講演会講演概要集 2015 (0), _1P1-N01_1-_1P1-N01_4, 2015

Living organisms are capable of self-reproduction, self-repair, and high adaptability by dynamically changing their control strategy and mechanical properties. By applying biological materials such …

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山蔦 栄太郎, ベー スーピン, 森島 圭祐 ロボティクス・メカトロニクス講演会講演概要集 2015 (0), _1P2-W07_1-_1P2-W07_2, 2015

...In our study, we used two types of Drosophila melanogaster (DM, fruit fly) larvae that express GCaMP calcium indicator or channelrhodopsin-2 (ChR2) in the cell membrane of skeletal muscles....

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Takaki Miyako, Goto Kei, Kawahara Isao, Nabekura Junich Ｊｏｕｒｎａｌ　ｏｆ　Ｓｍｏｏｔｈ　Ｍｕｓｃｌｅ　Ｒｅｓｅａｒｃｈ 51 (0), 82-94, 2015

...would perform functional studies of new neurons over the thick granulation tissue at anastomoses, using <i>in vivo</i> imaging with 2PM and double transgenic mice expressing a calcium indicator such as GCaMP6...

DOI Web Site PubMed ほか1件 参考文献30件

船水 章大, Kuhn Bernd, 銅谷 賢治 生体医工学 53 (Supplement), S130_02-S130_02, 2015

...investigate their neural implementation, mice were trained to do a virtual navigation task and neural activity was recorded in the posterior parietal cortex (PPC) with the genetically encoded calcium indicator GCaMP6f...

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Hailong Li, Xiaowan Wang, Nannan Zhang, Manoj K. Gottipati, Vladimir Parpura, Shinghua Ding Cell Calcium 56 (6), 457-466, 2014-12

DOI Web Site Web Site 被引用文献3件

Matthieu P. Vanni, Timothy H. Murphy The Journal of Neuroscience 34 (48), 15931-15946, 2014-11-26

...Cortical functional connectivity was evaluated using wide-field imaging in lightly anesthetized<jats:italic>Emx1-cre</jats:italic>X<jats:italic>Rosa26-GCaMP3</jats:italic>mice expressing calcium sensor...

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Christina K. Kim, Andrew Miri, Louis C. Leung, Andre Berndt, Philippe Mourrain, David W. Tank, Rebecca D. Burdine Frontiers in Neural Circuits 8 138-, 2014-11-26

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