A Novel Fluorogenic Substrate for Horseradish Peroxidase: Efficient Detection of Membrane-Bound Nucleic Acids and Simultaneous Detection of DNAs

• Fujita Satoshi

  Department of Applied Chemistry, Nagoya Institute of Technology

• Toru Takeshi

  Department of Applied Chemistry, Nagoya Institute of Technology

• Kondoh Yasumitu

  Aisin Cosmos R & D Co., Ltd.

• Kagiyama Naoto

  Aisin Cosmos R & D Co., Ltd.

• Momiyama Masayoshi

  Aisin Cosmos R & D Co., Ltd.

Abstract

Aniline derivatives were found to be trasformed to fluorescent compounds on treatment with horseradish peroxidase/H₂O₂. DNA was detectable with high resolution in the horseradish peroxidase-linked immunosobent fluorescence assay of the membrane-bound DNA using one of the aniline derivatives. A new method for a simultaneous detection of two different DNA sequences was also achieved.

Journal

• Chemistry Letters

  Chemistry Letters 26 (10), 1075-1076, 1997

  The Chemical Society of Japan

Keywords

• General Chemistry

Details

• CRID: 1390001204576458496
• NII Article ID: 10002616221
• NII Book ID: AA00603318
• DOI: 10.1246/cl.1997.1075
• COI: 1:CAS:528:DyaK2sXms1egsb0%3D
• ISSN: 13480715
• ISSN: 03667022
• Web Site: https://academic.oup.com/chemlett/article-pdf/26/10/1075/56074014/cl.1997.1075.pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed