Is Rabbit CLCA1 Related to the Basolateral Ca2+-Dependent Cl- Channel of Gastric Parietal Cells?
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- Sakai Hideki
- Department of Pharmaceutical Physiology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
Bibliographic Information
- Other Title
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- Is Rabbit CLCA1 Related to the Basolateral Ca〔2+〕-Dependent Cl〔-〕 Channel of Gastric Parietal Cells?
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Abstract
An expression of mRNA coding the calcium-activated Cl− channel-1 (CLCA1) in rabbit gastric parietal cells was examined to verify the possibility that the CLCA1 mediates housekeeping Cl− channels in the basolateral membrane. In whole-cell voltage-clamp experiments of rabbit parietal cells, A23187 (2 μM), a Ca2+ ionophore, activated the basolateral Cl− channels. The partial cDNA fragment of rabbit CLCA1 could be amplified from the total RNA of tracheal epithelium. A Northern blot analysis showed that rabbit CLCA1 mRNA of 3.4 kb is highly expressed in the tracheal epithelium, but not in the gastric parietal cells. Even in a more sensitive detection of rabbit CLCA1 mRNA by RT-PCR, no signal could be observed in the gastric parietal cells. These results suggest that the CLCA1 protein may not be a subunit of the housekeeping Ca2+-dependent Cl− channel in the basolateral membrane of rabbit gastric parietal cells.<br>
Journal
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- The Japanese Journal of Physiology
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The Japanese Journal of Physiology 51 (1), 121-125, 2001
THE PHYSIOLOGICAL SOCIETY OF JAPAN
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Keywords
Details 詳細情報について
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- CRID
- 1390282680017737856
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- NII Article ID
- 130004435757
- 10008293114
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- NII Book ID
- AA00691224
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- COI
- 1:CAS:528:DC%2BD3MXivVGktrs%3D
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- ISSN
- 18811396
- 0021521X
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- NDL BIB ID
- 5739253
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- PubMed
- 11282004
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed