Bitter Gourd Induces Apoptosis in HL60 Human Leukemia Cells and Suppresses the Production of Inflammatory Cytokine in RAW264.7 Macrophage Like Cells

• Kobori Masuko

  National Food Research Institute

• Amemiya Junko

  Fukushima Prefecture Agricultural Experiment Station

• Sakai Miho

  Miyazaki Prefectural Food Research & Development Center

• Shiraki Mitoshi

  Miyazaki Agricultural Experiment Station

• Sugishita Hiroyuki

  Miyazaki Agricultural Experiment Station

• Sakaue Naoko

  Ariake National Collage of Technology

• Hoshi Yoshikazu

  Kyushu Tokai University, School of Agriculture

• Yukizaki Chizuko

  Miyazaki Prefectural Food Research & Development Center

Bibliographic Information

Other Title

• ニガウリのがん細胞アポトーシス誘導効果および炎症性サイトカイン産生抑制効果
• ニガウリ ノ ガン サイボウ アポトーシス ユウドウ コウカ オヨビ エンショウセイ サイトカイン サンセイ ヨクセイ コウカ

Abstract

We previously demonstrated that bitter gourd (Momordica charantia L.) ethanol extract induced apoptosis in HL60 human leukemia cells. To examine the effect of different bitter gourd cultivars on cancer cell growth, we determined the effect of nine bitter gourd cultivars grown in Miyazaki prefecture on the growth of HL60 cells. Although growth inhibitory effect of seed extracts was stronger than that of pericarp or placenta extracts, all the extracts inhibited the growth of HL60 cells at concentrations of 25-200μg/ml. The extracts of pericarp, placenta and seeds of bitter gourds cultivars induced apoptosis in HL60 cells after 24h incubation.<BR>Suppression of inflammatory responses is expected to reduce inflammatory disease and development of cancer. Therefore, we determined the effect of the major bitter gourd cultivar ‘Sadowara 3’ on the bacterial lipopolysaccharide (LPS)-induced TNFα production from RAW264.7 mouse macrophage-like cells. Placenta extract inhibited the TNFα production induced by LPS ; however, it did not have any effect on the growth of RAW264.7 cells. The bitter gourd placenta is suggested to possess the suppressive effect on inflammatory responses.

Journal

• Nippon Shokuhin Kagaku Kogaku Kaishi

  Nippon Shokuhin Kagaku Kogaku Kaishi 53 (8), 408-415, 2006

  Japanese Society for Food Science and Technology

Keywords

• Food Science

Details

• CRID: 1390282681385421696
• NII Article ID: 10018217865
• NII Book ID: AN10467499
• DOI: 10.3136/nskkk.53.408
• ISSN: 18816681
• ISSN: 1341027X
• NDL BIB ID: 8015465
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8015465
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed