Characterization of Anti-peptide Antibody for Wheat Nonspecific Lipid Transfer Protein 1

• Oita Shigeru

  National Agricultural Research Center for Tohoku Region

• Yokota Satoshi

  National Agricultural Research Center for Hokkaido Region

Bibliographic Information

Other Title

• コムギＮｏｎｓｐｅｃｉｆｉｃ　Ｌｉｐｉｄ　Ｔｒａｎｓｆｅｒ　Ｐｒｏｔｅｉｎ　１抗ペプチド抗体の特性
• コムギ Nonspecific Lipid Transfer Protein 1 コウペプチド コウタイ ノ トクセイ

Abstract

To obtain a highly specific immunoglobulin G (IgG) for wheat nonspecific lipid transfer protein 1 (LTP), a wheat LTP peptide comprising amino acids Nos. 17-27 was designed as the antigen. Rabbit IgG showed high affinity to LTP of wheat and rye, but less or no affinity to water-extracted proteins of barley, oat, rice and maize. The LTP in 1μg of water-extracted wheat and rye protein was detectable by immunoblotting. Purified wheat LTP of over 2ng was detectable by immunoblotting and enzyme linked immunosorbent assay.

Journal

• Nippon Shokuhin Kagaku Kogaku Kaishi

  Nippon Shokuhin Kagaku Kogaku Kaishi 55 (4), 137-142, 2008

  Japanese Society for Food Science and Technology

Keywords

• wheat
• nonspecific lipid transfer protein
• anti-peptide antibody
• allergen

Details

• CRID: 1390282681385939200
• NII Article ID: 10021178454
• NII Book ID: AN10467499
• DOI: 10.3136/nskkk.55.137
• COI: 1:CAS:528:DC%2BD1cXmtVSlt7w%3D
• ISSN: 18816681
• ISSN: 1341027X
• NDL BIB ID: 9455559
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9455559
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed