書誌事項
- タイトル別名
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- Simultaneous determination of aspartame, its epimer and their degradation products in foods by HPLC and LC/MS
- HPLC オヨビ LC MS ニ ヨル ショクヒン チュウ ノ アスパルテーム アスパルテームエピマ オヨビ ソレラ ノ ブンカイブツ ノ ドウジ ブンセキ
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A simple and rapid method has been developed for determining of L,L-aspartame (APM), its epimer (L,D-APM), diketopiperazine (DKP), L-phenylalanine (Phe) and D-Phe in various foods by high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). These compounds in foods were extracted with 0.01mol/L hydrochloric acid in ultrasonic bath, and the extract was loaded onto a strata-X-C, cation-exchange and reverse-phase cartridge. The HPLC separation was performed on a SUMICHIRAL OA-5000 (monolithic-type, 4.6mm i.d.×10cm) with ultraviolet detection, using 2mmol/L CuSO4 solution-acetonitrile-isopropyl alcohol (85:10:5) as a mobile phase. The LC/MS separation was performed on a CHIROBIOTIC TAG (2.1mm i.d.×25cm, 5μm) with a mobile phase of 0.01% ammonium acetate-0.005% acetic acid-ethanol (1:1:2). The recoveries of 5 compounds from foods added at the level of 0.2g/kg in HPLC and LC/MS were 93.9-102.9% and 82.1-102.4%, respectively. In HPLC, the detection limits of APM, DKP and Phe were 0.02g/kg. In LC/MS, the detection limits of APM was 0.01g/kg, and DKP and Phe were 0.02g/kg.
収録刊行物
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- 日本食品化学学会誌
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日本食品化学学会誌 17 (2), 130-135, 2010
日本食品化学学会
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詳細情報 詳細情報について
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- CRID
- 1390001205196622080
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- NII論文ID
- 110007700656
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- NII書誌ID
- AA11666400
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- ISSN
- 21896445
- 13412094
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- NDL書誌ID
- 10827792
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可