新しい緑膿菌選択分離培地

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書誌事項

タイトル別名
  • Quinine-Nalidixic acid Agar
  • 新しい緑膿菌選択分離培地-キニーネ・ナリジキシン酸寒天-について
  • アタラシイ リョクノウキン センタク ブンリバイチ-キニーネ ナリジキシンサン カンテン- ニ ツイテ
  • A new selective isolation medium for <I>Pseudomonas aeruginosa</I>
  • キニーネ・ナリジキシン酸寒天-について

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抄録

A new composition was devised for the selective isolation of Pseudomonas aeruginosa. This medium is composed of 0.5% sodium glutamate, 0.3%β-alanine, 0.2% yeast extract, 0.1% diammonium phosphate, 0.03% monopotassium phosphate, 0.1% magnesium sulfate, 0.5% sodium chloride, 0.1% quinine hydrochloride, 0.001% nalidixic acid and 1.5% agar, the reaction being adjusted at pH 7.0. All ingredients are suspended in appropriate amount of distilled water, heated to dissolve completely, and sterilized by heating at 121°C for 15 minutes.<BR>In this composition quinine serves as a chief selective agent, and nalidixic acid acts as an additive agent to suppress the overgrowth of some species of Enterobacteriaceae such as E. coli, Enterobacter, Proteae, Klebsiella and Serratia. The use of peptone as the nutrient source caused the decrease in the selective action of quinine.β-alanine, Mg++ ion and phosphate were helpful for the pigmentation of Ps. aeruginosa colonies.<BR>This composition (quinine-nalidixic acid agar) was tested for its selectivity as compared with NAC agar (nalidixic acid-cetrimide agar) and SS agar using clinical specimens. For the isolation of Ps. aeruginosa from 1, 237 fecal samples, the rate of isolation was 9.5% with both NAC agar and quininenalidixic acid agar, being higher by far than SS agar, whereas in the test from 327 otolaryngeal samples, the rate of isolation of Ps. aeruginosa was nearly equal to that with 3 sorts of selective media. Quininenalidixic acid agar was established to be superior to NAC agar in the pigment production of Ps. ageruginosa strains.<BR>From these experimental results quinine-nalidixic acid agar is thought to be useful for the clinical investigations of Ps. aeruginosa.

収録刊行物

  • 感染症学雑誌

    感染症学雑誌 44 (2), 117-127, 1970

    一般社団法人 日本感染症学会

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