ムンプス赤血球凝集抑制 (HI) 抗体測定法の改良とムンプス生ワクチン (星野株) の接種試験について

DOI Web Site PubMed
  • 垣花 淳一
    昭和大学医学部大学院医学研究科内科系小児科学 北里研究所ウイルス部

書誌事項

タイトル別名
  • Trial Inoculation of A Hoshino Strain Live Attenuated Mumps Virus Vaccine and A Brief Study on the Mumps Hemagglutination-Inhibition Test
  • ムンプス セッケッキュウ ギョウシュウ ヨクセイ HI コウタイ ソクテイホウ

この論文をさがす

抄録

Hoshino strain live attenuated mumps virus vaccine (A chick embryo tissue culture type) has been developed by Makino et al. In this study, this viral strain of the 7 th subculture was used as the seed virus for the preparation of vaccine Lot No.TC-2. This vaccine was inoculated subcutaneously into children with no history of mumps infection and the resulting clinical reactions and antibody responses were investigated.<BR>In addition, in view of that the hemagglutination-inhibition (HI) test for mumps, presently available Japan NIH method, does not adequately remove non-specific inhibitors (NsI) with the consequent danger of obtaining positive reactions even with actually negative sera, the test methodology was also studied with a intention to improve it by increasing its sensitivity.<BR>The following results were. obtained:<BR>1) In the present HI test for mumps, kaolin is used to remove NsI from test sera. The extent of removal, however, varies with the product lot. RDE (products of Takeda Chemical Industries, Ltd. Japan) was found to have high removal efficiency which did not vary by lot. In addition, when the adsorption of hemagglutinin and test human serum, carried out at 37°C for 1 hour according to the present method, is prolonged by overnight standing at 4°C, an increase in HI titer results. Hemagglutinin purified with ether or Tween 80 results in a higher rate of positive HI determinations and the HI titers increase.<BR>2) When Hoshino vaccine was inoculated into 128 children with no history of mumps infection, no clinical reactions were observed in any of the vaccinees. At 6-8 th week post vaccination, the antibody levels in 35 initially seronegative children were studied with the following results. The seroconversion rates and geometric mean titers, respectively, were: HI antibodies: 80%(28/35), 5 × 21.4; neutralizing antibodies: 92.9%(26/28), 23.0; CF-antibodies for S-antigen: 96.9%(31/32), 21.9.<BR>From the above results, it was concluded that the seed virus used in producing this test vaccine was a strain of attenuated mumps virus which was sufficiently immunogenic without producing undesirable clinical reactions.

収録刊行物

  • 感染症学雑誌

    感染症学雑誌 50 (4), 145-152, 1976

    一般社団法人 日本感染症学会

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ