加熱損傷を受けた腸管出血性大腸菌Ｏ１５７の増菌培養の基礎的検討

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  • Basic Examination of the Enrichment Culture for <I>Escherichia coli</I> O157: H7 Injured by Heating
  • カネツ ソンショウ オ ウケタ チョウカン シュッケツセイ ダイチョウキン O157 ノ ゾウキン バイヨウ ノ キソテキ ケントウ

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As a part of the basic research for the improvement of the method for detection of Shigalike toxin producing Escherichia coli O157: H7 (hereinafter called “E. coli O157”) from raw meat, we examined the enrichment culture method for E. coli O157 injured by heating, by combination of culture medium (mEC-Nb, TSB), incubation time (5, 10, 20 hr), temperature (37°C, 42°C), and method (static, shaken). The examination also tested the efficacy of novobiocin for some species of bacteria which compete with E. coli O157.<BR>The results of the examination are as follows.<BR>1) It was shown that the enrichment from very few (10-3 cfu/ml) E. coli O157 injured by heating to 106 cfu / ml or more in an enrichment culture using mEC-Nb medium prepared by the official method took 20 hours, regardless of the incubation temperature or method.<BR>2) After 10 hours or more of enrichment in TSB, the cfu count of E. coli O157 injured by heating was greater at 42°C than at 37°C, irrespective of the incubation method.<BR>3) It was confirmed that the growth of bacteria which would compete with E. coli O157 in the enrichment was inhibited by the addition of 20 mg/l novobiocin to the culture medium.

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