Amperometric Detection of Oxidized and Reduced Glutathione at Anodically Pretreated Diamond Electrodes

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  • Chiaki Terashima
    Department of Applied Chemistry, School of Engineering, The University of Tokyo7-3-1 Hongo, Bunkyo-ku, Tokyo
  • Tata N Rao
    Department of Applied Chemistry, School of Engineering, The University of Tokyo7-3-1 Hongo, Bunkyo-ku, Tokyo
  • Bulusu V Sarada
    Department of Applied Chemistry, School of Engineering, The University of Tokyo7-3-1 Hongo, Bunkyo-ku, Tokyo
  • Akira Fujishima
    Department of Applied Chemistry, School of Engineering, The University of Tokyo7-3-1 Hongo, Bunkyo-ku, Tokyo

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Abstract

<jats:title>Abstract</jats:title> <jats:p>Anodically oxidized diamond electrodes have enabled direct oxidation of not only thiols but also disulfides such as oxidized glutathione. Coupled with LC separation after sample pretreatment, simultaneous determination of oxidized and reduced glutathione in rat whole blood was achieved at 1.50 V vs Ag/AgCl. The present method was comparable with enzymatic assay in real sample analysis.</jats:p>

Journal

  • Chemistry Letters

    Chemistry Letters 32 (2), 136-137, 2003-01-17

    Oxford University Press (OUP)

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